What Are the Effects of Polarity Reversal During Protein Electrophoresis?
In protein electrophoresis, reversing the polarity of the power supply leads to the reverse migration of proteins. Under normal conditions, proteins migrate from the cathode (negative electrode) to the anode (positive electrode) due to the applied electric field. However, if the polarity is reversed, the direction of the electric field is also inverted, causing proteins to migrate in the opposite direction. As a result, proteins may move out of the gel and diffuse into the buffer, preventing proper separation and analysis.
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