What Current, Time, and PVDF Membrane Requirements are Needed for 17 kDa Protein Transfer in Western Blot?
In Western blot membrane transfer, the current and time settings are determined by the size and properties of the protein being transferred. For a 17 kDa protein, the following guidelines are recommended:
1. Current Setting
A lower current is typically applied to minimize protein thermal denaturation and the evaporation of electrophoresis buffer. For small molecular weight proteins, the common current setting is between 20–30 mA.
2. Transfer Time
The transfer time is also dependent on the protein size. For a 17 kDa protein, the typical transfer time ranges from 30 to 60 minutes.
3. PVDF Membrane Requirements
PVDF membranes with a pore size of either 0.2 μm or 0.45 μm are commonly recommended.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
Submit Inquiry
How to order?
