De Novo Peptide Sequencing Service

There are two common methods for peptide sequence analysis: database search and de novo sequencing. Database searches identify peptide sequences by comparing them in known databases. However, due to incomplete databases for some species, many new or bioactive peptides cannot be matched. De novo peptide sequencing addresses this issue by using tandem mass spectrometry (MS/MS) to determine the amino acid sequence of a peptide without a sequence database. This method allows for the analysis of new and unknown peptides regardless of the availability of theoretical databases.

 

The basic principle of de novo sequencing involves calculating the mass of amino acid residues in the peptide chain by measuring the mass difference between two fragment ions. The development of de novo peptide sequencing technology is closely linked to advances in MS. In recent years, the resolution of MS has significantly improved, particularly with high-precision instruments, providing a solid foundation for peptide sequence analysis.

 

MtoZ Biolabs utilizes Thermo's latest Obitrap Fusion Lumos mass spectrometer, combined with Nano-LC nanoliquid chromatography technology, to offer MS-based de novo peptide sequencing services. The Obitrap Fusion Lumos, known for its high resolution and sensitivity, ensures the detection of low-abundance peptide fragments. The integration of HCD and ETD fragmentation modes maintains the integrity of peptide fragments. After acquiring raw mass spectrometry data, the peptide sequence is deduced using de novo sequencing techniques.

 

Sample Submission Requirements

Please prepare samples of at least 20 µg, either in solution or lyophilized powder form. For solution samples, ensure the buffer free of high salts, SDS, and other detergents. MtoZ Biolabs can analyze peptide from both liquid and solid samples. Solid samples should be transported in ice packs, while solution samples can be vacuum-dried or freeze-dried and then transported in ice packs or on dry ice. Free project evaluation, welcome to learn more details!