Resources

• • Application of N-Glycosylation Site Analysis in Biopharmaceutical Development

  N-glycosylation refers to the process of adding sugar chains to the nitrogen atom of proteins, which significantly impacts their structure and function. In biopharmaceutical development, the analysis of N-glycosylation sites provides crucial insights into understanding drug targets' functionality, enhancing drug stability and biocompatibility, and optimizing production processes.

• • Workflow of N-Glycosylation Profiling in Biopharmaceuticals

  N-glycosylation is an essential form of post-translational modification of proteins that significantly impacts the quality and bioactivity of biopharmaceuticals. Understanding the workflow of N-glycosylation is crucial for ensuring the safety and efficacy of biologics.

• • Advantages and Disadvantages of Mass Spectrometry-Based N-Glycosylation Analysis

  N-glycosylation is a widely occurring post-translational modification in living organisms, significantly impacting protein function, stability, and various biological processes such as cell signaling. Mass spectrometry (MS)-based N-glycosylation analysis has emerged as a crucial research tool in this field due to its high sensitivity and throughput.

• • Principle of N-Glycosylation Site Identification

  N-glycosylation refers to the process of attaching glycan chains to the nitrogen atom of asparagine residues in proteins. This modification plays a crucial role in cellular functions and signaling pathways. Understanding the recognition of N-glycosylation sites is essential for elucidating protein functions, interactions, and their roles within biological systems.

• • Mechanism of HILIC-UHPLC in N-Glycosidic Bond Detection

  In biological molecules, carbohydrates play a crucial role as important modifying groups in the functions and structures of proteins. N-glycosidic bonds refer to the connection of carbohydrates to the nitrogen atom of proteins or other biomolecules through glycosidic bonds. Accurate detection and analysis of N-glycosidic bonds is essential for studying glycobiology, glycomics, and related diseases such as diabetes and cancer.

• • Workflow of HILIC-UHPLC in N-Glycosidic Bonds Analysis

  N-glycosidic bonds play a crucial role in various biological processes and diseases, making their analysis essential for understanding carbohydrate biology. High-performance liquid chromatography (HPLC) combined with hydrophilic interaction chromatography (HILIC) provides an effective method for the separation and analysis of N-glycans.

• • Application of HILIC-UHPLC in N-Glycan Structural Analysis

  N-glycans are essential carbohydrate molecules found in glycoproteins and glycolipids, playing crucial roles in various biological processes such as cell recognition, signal transduction, and immune response. In recent years, the importance of structural analysis of N-glycans has increased with the in-depth study of glycoscience.

• • Advantages and Disadvantages of HILIC-UHPLC in N-Glycan Detection

  In biochemical and glycomic research, the analysis of N-linked glycans (N-glycans) is crucial. The structure and function of N-glycans significantly impact physiological processes in living organisms, making the development of efficient and sensitive analytical methods essential. In recent years, Hydrophilic Interaction Liquid Chromatography-Ultra High Performance Liquid Chromatography (HILIC-UHPLC) has emerged as a promising separation technique for N-glycan detection, showcasing unique advantages.

• • Principle of HILIC-UHPLC in N-Glycosidic Bonds Analysis

  With the deepening of research into glycoscience, the analysis of N-glycans has garnered increasing attention in the fields of biomedicine, drug development, and biotechnology. N-glycans, as modifications of glycan chains, are widely present in various proteins and lipids, and changes in their structure are closely related to multiple diseases, such as cancer and diabetes. Therefore, developing efficient analytical methods is crucial for uncovering their biological functions and related mechanisms.

• • Mechanism of O-Glycan Profiling

  O-glycans are carbohydrate chains formed through glycosylation reactions with hydroxyl amino acid residues, widely present in various proteins, especially in membrane and secreted proteins. Analyzing O-glycans is crucial for understanding their biological functions, structural characteristics, and roles in diseases.