Resources

Proteomics Databases



Metabolomics Databases

• • TMT Quantification

  TMT quantification (Tandem Mass Tag quantification) is a widely employed high-throughput technique in proteomics that enables relative quantification of proteins or peptides across multiple samples through isotopic labeling. This approach utilizes tandem mass spectrometry (MS/MS) to compare protein expression levels with high precision by incorporating distinct TMT tags into each sample. A key advantage of TMT quantification lies in its ability to analyze multiple samples simultaneously within a singl......

• • Advances in N Glycan Analysis Techniques

  N-glycan analysis is the systematic study of N-linked glycosylation modifications on proteins. N-glycans are attached to asparagine (Asn) residues via glycosidic bonds, with a typical structure initiated by N-acetylglucosamine (GlcNAc). These sugar chains can undergo further modifications and branching, forming complex polysaccharide structures. Glycosylation is a dynamic and reversible modification influenced by factors such as enzymatic activity, intracellular conditions, and pathological changes. D......

• • TurboID Proximity Labeling

  TurboID proximity labeling is an advanced technology designed to investigate protein-protein interactions and their spatial organization within cells. The technique operates by fusing a target protein with the TurboID tag, a highly efficient biotin ligase. In the presence of biotin, TurboID rapidly biotinylates proteins in close proximity, typically within minutes. These biotin-labeled proteins can then be purified using affinity-based methods, such as streptavidin-biotin capture, and identified throu......

• • Single-Cell TCR Sequencing

  Single-cell TCR sequencing is a genomic technique designed to characterize T cell receptor (TCR) sequences at single-cell resolution. T cell receptors, expressed on the surface of T cells, are responsible for antigen recognition and immune response initiation. The diversity of TCRs underpins the ability of T cells to recognize a wide array of antigens. Analyzing TCR sequences offers valuable insights into immune system functionality and disease mechanisms.   In tumor immunology, single-cell TCR sequen......

• • Protein Conservation Analysis

  Protein conservation analysis is a bioinformatics approach aimed at examining the similarities and differences in protein sequences across various organisms. At its core, this method involves comparing amino acid sequences to pinpoint regions that have remained unchanged throughout evolution and those that have undergone alterations. Evolutionarily conserved regions are often tightly linked to the structural integrity and biological functions of proteins, making them critical subjects for investigatio......

• • BioID Proximity Labeling

  BioID proximity labeling is a powerful bioinformatics tool designed to investigate intracellular protein interactions and protein-protein interaction networks. This method involves tagging target proteins with a low-molecular-weight biotin-labeling enzyme, such as BirA, which catalyzes biotinylation in the presence of biotin. During this process, biotin is covalently attached to proteins located in close proximity to the target protein. These biotinylated proteins can then be isolated through affinity......

• • Determining Amino Acid Sequence

  Determining amino acid sequence is a critical technique used to elucidate the arrangement of amino acids within a protein. Proteins are essential molecules that carry out a wide range of functions in living organisms. They consist of amino acids linked by peptide bonds, and the specific amino acid sequence dictates both the three-dimensional structure and biological function of the protein. This technique enables researchers to gain detailed insights into the functional mechanisms of proteins, particu......

• • Protein Biomarker Discovery

  Protein biomarker discovery aims to provide vital tools for disease diagnosis, treatment, and prognosis by identifying and validating changes in protein profiles under specific biological conditions. Protein biomarkers are molecular indicators in organisms that reflect physiological or pathological states. These biomarkers are invaluable in biomedical research as they facilitate early disease detection, disease progression assessment, treatment monitoring, and the development of novel therapeutic stra......

• • Protein Ligand Interaction Profile

  Protein ligand interaction profile is a critical approach for studying the relationships between proteins and their interacting molecules in biological systems. This analysis is focused on understanding the interaction processes between proteins and their ligands, which can include small molecules, ions, and biomacromolecules such as drug compounds, metabolites, and nucleic acids. These interactions are crucial in regulating biological processes, including signal transduction, metabolic control, and g......

• • LC-MS Amino Acid Analysis

  LC-MS amino acid analysis is a powerful analytical method that integrates liquid chromatography (LC) and mass spectrometry (MS) to accurately identify and quantify amino acids in samples. The technique combines LC's ability to separate complex sample components with MS's capability to detect and quantify these components, resulting in high sensitivity, resolution, and versatility. This makes LC-MS amino acid analysis especially effective for examining complex samples with low amino acid concentrations......