Resources

Proteomics Databases



Metabolomics Databases

• • How to Identify the Structure of Peptides?

  Peptides are a class of compounds consisting of 11 to 50 amino acids connected by peptide bonds. They differ from proteins only in the length of the peptide chain, with no strict distinction between the two. Like proteins, peptides also have four structural levels: primary structure, secondary structure, tertiary structure, and quaternary structure. The primary structure refers to the arrangement of amino acids from the N-terminus to the C-terminus of the peptide molecule.

• • Protein Extinction Coefficient: Key Parameter in Bioanalysis

  The molar extinction coefficient of a protein is a parameter that measures the protein's ability to absorb light at a specific wavelength. It is crucial for biological analysis, particularly in the determination of protein concentration, evaluation of protein purity, and study of protein interactions with other molecules.

• • How to Detect Protein Ubiquitination-IP

  Protein Ubiquitination is a crucial cellular biological process. It involves the binding of Ubiquitin protein to the target protein, thereby regulating the degradation, positioning, activation, etc., of the target protein. To detect protein ubiquitination, methods such as Immunoprecipitation (IP) combined with Western blot, mass spectrometry analysis, etc., are commonly used. Here are the typical steps for protein ubiquitination IP.

• • Chromatin Methylation Sequencing

  Histone methylation sequencing is a technique used to detect and analyze methylation modifications on histones. Methylation, especially on histone lysine and arginine residues, is critical for regulating gene expression and cell function. This sequencing technique helps us understand how chromatin structure affects gene activation and silencing.

• • What Is Used to Enrich Ubiquitin Proteins?

  Commonly used ubiquitinated protein enrichment methods mainly include: Ubiquitin Antibody Enrichment This is one of the most common methods for enriching ubiquitinated proteins. Researchers use high-affinity ubiquitin antibodies to bind to ubiquitinated proteins, then use methods such as immunoprecipitation or affinity chromatography to bind the ubiquitinated proteins to the antibodies, which can then be used for further analysis, such as mass spectrometry identification or protein affinity experiments.

• • Considerations for MS Identification of Phosphorylation Sites

  Phosphorylation is a common type of protein post-translational modification (PTM), playing a significant role in biological processes such as cell signal transduction, protein activity and stability regulation. Mass spectrometry is one of the main methods for identifying protein phosphorylation, but the following issues need to be considered in the actual operation:

• • How to Detect the Protein Ubiquitination Level via Western Blot?

  Western Blot Detection of Protein Ubiquitination Level Western blot is a commonly used method for protein detection, which can accurately detect the presence and expression level of specific proteins in samples, as well as post-translational modifications of proteins, such as phosphorylation and ubiquitination. This article will mainly introduce how to use Western blot to detect the ubiquitination level of proteins.

• • Yeast Proteomics Analysis Strategy

  In the field of biotechnology and molecular biology, yeast (especially the brewing yeast Saccharomyces cerevisiae) has become an extremely important model organism due to its ease of cultivation, relatively simple genome, and remarkable similarity to higher organisms. It is not only widely used in industrial production and fermentation processes, but also provides an outstanding model for understanding the biological processes of more complex organisms.

• • AI and Multiplex Cloning Redefine Aantibody Discovery

  Antibodies, as important biopharmaceuticals, play a significant role in the treatment of cancer, autoimmune diseases, and infectious diseases. Antibody discovery, as a crucial process in antibody drug design, has witnessed the application of numerous new technologies. However, antibody research still faces multiple challenges such as affinity, specificity, stability, and immunogenicity.

• • Lipidomics Study of Escherichia Coli Metabolism

  In modern life science research, lipidomics has become an indispensable technique for understanding cell functions, disease mechanisms, and drug action mechanisms. Lipids, as important components in organisms, not only play a key role in the structure and function of cell membranes, but also participate in various biological processes such as energy storage, signal transduction, and cell death.