Resources

• • Mass Spectrometry for Protein Identification

  Mass spectrometry for protein identification is a widely-used technique that enables the identification and quantification of proteins within a sample. Its applications span biological research, clinical diagnostics, and drug discovery. In this process, mass spectrometry for protein identification plays a crucial role in discovering novel proteins, exploring protein modifications, and analyzing protein interactions and functions.   The procedure for Mass Spectrometry for Protein Identification consist......

• • What Aspects of Proteins Can be Measured Using Mass Spectrometry?

  Mass spectrometry (MS) is a versatile analytical technique widely used in proteomics to measure various aspects of proteins. It has revolutionized our ability to identify and quantify proteins, assess post-translational modifications (PTMs), and investigate complex protein interactions.   Protein Identification Mass spectrometry facilitates the identification of proteins by analyzing the mass-to-charge ratio (m/z) and fragmentation spectra of peptides or proteins, allowing precise identification of pr......

• • Mass Spectrometry Analysis Workflow

  The mass spectrometry analysis workflow is a comprehensive methodology for determining molecular mass, structural features, and composition, making it indispensable in fields like chemistry, biology, pharmacology, and environmental science. In proteomics, it serves as a cornerstone for protein identification and the analysis of protein interactions. This workflow encompasses six main stages:   Sample Preparation 1. Purification Isolating target molecules from complex biological or chemical matrices en......

• • Proteomics Research Using Mass Spectrometry

  Proteomics research using mass spectrometry (MS) has become indispensable for identifying and quantifying cellular proteins, offering insights into their functions and interactions. As a cornerstone of biomedical innovation, mass spectrometry has propelled advancements in understanding disease mechanisms, biomarker discovery, and drug development.   Applications of Mass Spectrometry in Proteomics Proteomics research using mass spectrometry encompasses several critical applications:   1. Protein Identi......

• • Mass Spectrometry in Protein Analysis

  Mass spectrometry in protein analysis has become indispensable for understanding complex cellular processes at the molecular level. Mass spectrometry is a pivotal technique in protein analysis, providing essential information on the mass, structure, composition, and quantification of proteins. Through mass spectrometry analysis, researchers can identify unknown proteins, characterize protein complexes, detect post-translational modifications, quantify protein levels, and investigate protein dynamics. ......

• • Mass Spectrometry-Based Proteomics and Metabolomics Analysis Techniques

  Mass Spectrometry-Based Proteomics and Metabolomics Analysis Techniques are two fundamental branches of systems biology that are widely used in biomedical research, disease mechanism exploration, drug development, and biomarker discovery. Both rely on mass spectrometry (MS) to identify and quantify biomolecules within biological samples; however, they focus on different types of biomolecules. While proteomics investigates proteins and their modifications, interactions, and functions, metabolomics focu......

• • Mass Spectrometry in Protein Analysis: New Insights for Biopharmaceuticals and Disease Treatment

  Mass Spectrometry is an analytical technique based on molecular mass and relative abundance, which enables the determination of molecular composition, structure, and quantity. This method involves ionizing the target molecules and analyzing them in a mass spectrometer, generating a mass spectrum that provides detailed information about the molecules.   Types of Mass Spectrometry This section introduces several widely used mass spectrometry techniques, including Proton Transfer Reaction Mass Spectromet......

• • Utilization of Unbiased Protein Identification by Mass Spectrometry in Biopharmaceutical Research

  Unbiased protein identification by mass spectrometry is a high-throughput method for protein identification that leverages mass spectrometry analysis. The technique involves isolating proteins from biological samples and analyzing them through mass spectrometry to identify and quantify the protein components present. Compared to traditional methods, this approach offers superior accuracy and sensitivity, with the added advantage of simultaneously identifying a large number of proteins.   Principle of ......

• • Mass Spectrometry and Proteins

  Mass spectrometry analysis has been widely employed in various research areas, including the identification and quantification of proteins, the analysis of epigenetic modifications, and the investigation of protein interaction networks. This technique allows for the precise measurement of the mass of proteins or peptides, facilitating the determination of their structure and composition.   Basic Principles of Mass Spectrometry and Proteins Analysis 1. Sample Preparation Protein samples are usually sub......

• • Protein Analysis via Mass Spectrometry

  Protein analysis via mass spectrometry is a widely used technique in proteomics and includes the following steps: Sample Preparation Proteins are extracted and purified through methods such as gel electrophoresis and liquid chromatography.   Protein Digestion Proteins are enzymatically digested into peptide fragments, typically using enzymes like trypsin.   Mass Spectrometry Analysis The peptide fragments are ionized, with common methods being MALDI and ESI. Their mass-to-charge ratio (m/z) is then me......