Resources

Proteomics Databases



Metabolomics Databases

• • What Detector Is Used for Mass Spectrometry in Proteomics

  Mass spectrometry has emerged as a key research area in proteomics. The identification, quantification, and analysis of proteins in complex biological samples rely on mass spectrometry. Within mass spectrometry applications, the selection of an appropriate detector is particularly critical. Electron Multiplier: The electron multiplier is a traditional detector in mass spectrometry, known for its high sensitivity through signal amplification via electron multiplication. It utilizes a high-voltage electric...

• • Does Protein Degradation Affect Mass Spectrometry Identification

  Mass Spectrometry for Protein Analysis is a crucial tool in biological research, allowing researchers to determine the composition and structural characteristics of proteins. However, protein degradation is a prevalent challenge that can significantly affect the accuracy of mass spectrometry-based protein identification. This article examines the impact of protein degradation on mass spectrometry analysis and discusses strategies to mitigate its effects.

• • Precautions and Challenges in Protein Ubiquitination Detection Experiments

  Protein ubiquitination is a post-translational modification process involving the sequential attachment of the small protein ubiquitin to target proteins. This process plays a crucial role in various biological events, including the cell cycle, signal transduction, immune responses, and protein degradation. However, studying protein ubiquitination presents several challenges, as discussed in this paper.

• • Histone Methylation Modification Types in Plants

  In botanical research, DNA methylation is a crucial epigenetic modification that regulates gene expression, maintains chromatin stability, and influences various biological processes such as development. In plant genomes, DNA methylation primarily occurs at the fifth carbon position of cytosine (C). Based on the sequence context following the cytosine, DNA methylation can be classified into three types:

• • Post-Translational Modification Proteomics Analysis

  Post-translational modification proteomics (PTM proteomics) refers to the comprehensive profiling of proteins in organisms, tissues, or cells that undergo post-translational modifications. These chemical alterations include, but are not limited to, phosphorylation, ubiquitination, acetylation, methylation, and glycosylation. Such modifications significantly enhance the functional diversity of proteins and play pivotal roles in numerous biological processes, including signal transduction, regulation of......

• • Mass Spectrometry Analysis of HLA Peptides

  Mass spectrometry is an analytical technique used to detect and characterize substances by measuring the mass and relative abundance of ionized molecules. In immunological research, it serves as a robust tool for both qualitative and quantitative analysis of peptides associated with Human Leukocyte Antigens (HLA). This paper provides a concise overview of the application of mass spectrometry in studying HLA peptides.

• • Is Protein Purification Required Before Mass Spectrometry

  Mass spectrometry analysis generally requires protein purification. This is because the results of mass spectrometry can be influenced by other substances in the sample, such as salts or elution buffers, which may interfere with the proteins and distort the mass spectrometry results. To achieve accurate mass spectrometry data, it is therefore essential to purify the proteins from the sample. Several methods exist for protein purification, including centrifugation, electrophoresis, and chromatography.

• • How Many Blood Proteome Samples Are Generally Needed for Detection

  Blood proteomics is the scientific study focused on analyzing the complete set of proteins present in whole blood, plasma, serum, or blood cells. The determination of the required number of samples for a study in blood proteomics is largely dependent on the specific aims and methodologies of the research. For instance, exploring biomarkers for rare diseases across large populations may necessitate hundreds to thousands of samples, whereas research on common diseases might require only a few dozen samples.

• • What Measurements Does Proteomics Include

  Proteomics is the scientific discipline focused on the large-scale study of proteins, particularly their structures, functions, expression patterns, and interactions within cells, tissues, or entire organisms. The ultimate aim is to obtain a comprehensive understanding of these proteins. Proteomics involves various analytical and technological methodologies, as highlighted below.

• • Proteomics Commonly Used Mass Spectrometry

  Proteomics is a field of study focused on understanding the structure and function of the entire proteome. This encompasses various aspects such as protein expression, post-translational modifications, interactions, and functional roles. The primary technique employed in proteomics research is mass spectrometry, a method capable of analyzing the molecular mass, structural characteristics, and chemical modifications of proteins.