Resources
Proteomics Databases
Metabolomics Databases
-
In the post-genomic era, post-translational modifications (PTMs) have attracted increasing attention as critical regulatory mechanisms underlying protein functional diversity. Among these, acylation, particularly lysine acetylation, propionylation, butyrylation, and malonylation, represents an important class of lysine modifications that are extensively involved in the regulation of key biological processes, including metabolism, signal transduction, and chromatin remodeling. However, these modificati......
-
• Membrane Proteomics for Accelerating Drug Target Discovery
Membrane proteins play a critical role in cellular physiology and signal transduction and represent one of the most important classes of therapeutic targets in contemporary drug development. However, systematic studies of membrane proteins have long been hindered by their hydrophobic nature, low abundance, and structural complexity. With the advancement of membrane proteomics technologies, researchers can now systematically and in a high-throughput manner investigate the functional roles of membrane p......
-
• TMT vs Label-Free Quantitative Proteomics
Proteomics, as a pivotal tool for elucidating biological mechanisms, is transitioning from a discovery-driven approach to a phase of precise quantification. Among large-scale quantitative strategies, Tandem Mass Tag (TMT) labeling and Label-Free Quantification (LFQ) are the most widely adopted. Although both rely on high-resolution mass spectrometry platforms, they exhibit fundamental differences in experimental design, data quality, and suitable application scenarios. What is TMT-Based Quantificatio......
-
• Which Technologies Are Most Suitable for Spatial Proteomics Data Acquisition?
Spatial proteomics has emerged as a rapidly advancing frontier in life sciences, aiming to decipher the spatial distribution and dynamic alterations of proteins within tissues or cellular microenvironments. To fulfill this objective, data acquisition technologies must integrate high spatial resolution, robust protein detection throughput, and precise quantification. Currently, mainstream spatial proteomic acquisition approaches fall into two broad categories: imaging-based techniques and mass spectrom......
-
• An Overview of Protein–Protein Interaction Analysis Techniques
Protein–protein interactions (PPIs) are fundamental to virtually all cellular processes. From signal transduction and metabolic regulation to structural maintenance and immune responses, cellular functionality critically depends on the specificity and dynamic regulation of protein associations. Comprehensive analysis of PPIs not only enhances our understanding of molecular mechanisms under physiological conditions but also identifies key regulatory nodes involved in disease onset and progression. Eluc......
-
• What Is the Difference Between Spatial Proteomics and Spatial Transcriptomics?
Spatial proteomics and spatial transcriptomics are two fundamental technologies in the field of spatial omics. Both aim to characterize the molecular distribution within cells and tissues in a spatially resolved manner, but they differ in the types of biomolecules analyzed and their technical strategies. Definitions and Research Objects In summary: Spatial proteomics reveals what cells are doing at specific locations. Spatial transcriptomics reveals what cells are preparing to do at specific......
-
• Differences Between Peripheral and Integral Membrane Proteins and Methods for Their Identification
Membrane proteins and integral membrane proteins are critical components of the cellular membrane system, playing essential roles in signal transduction, molecular transport, and cell recognition. Although both types of proteins are associated with biological membranes, they differ significantly in structural characteristics, membrane association mechanisms, and functional properties, resulting in distinct experimental identification strategies. Differences Between Membrane Proteins and Integral Memb......
-
• Increasing Sensitivity and Coverage in Spatial Proteomics
Spatial proteomics integrates proteomics with spatial tissue information, aiming to generate high-resolution maps of protein expression and localization at the subcellular and sub-tissue scale. In research areas such as the cancer microenvironment, neurodegenerative diseases, and developmental biology, spatial proteomic data can uncover biological mechanisms that are often inaccessible to conventional bulk proteomics. However, spatial proteomics currently faces two fundamental challenges: Insufficient......
-
• Mass Spectrometry-Based Spatial Proteomics: Methods, Challenges, and Applications
Spatial proteomics, an emerging technology, integrates the quantitative capabilities of mass spectrometry-based proteomics with the preservation of tissue spatial information, redefining our understanding of tissue heterogeneity, cell–microenvironment interactions, and the spatial progression of diseases. Unlike traditional linear extract-and-analyze workflows, spatial proteomics aims for a three-dimensional understanding of in situ expression, local distribution, and spatial correlation. This approac......
-
• Types and Functions of Histone Post‑Translational Modifications
In eukaryotic cells, DNA is not present in a free or naked form within the nucleus but is instead highly organized, wrapped around histone octamers to form nucleosomes, which in turn assemble into the chromatin architecture. Beyond serving as a structural scaffold for DNA, histones play essential regulatory roles, particularly through a diverse array of post-translational modifications (PTMs) that precisely modulate chromatin dynamics and gene expression. Histone PTMs constitute a central mechanism of......
How to order?
