Resources
Proteomics Databases
Metabolomics Databases
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• MALDI-TOF Protein Sequencing
MALDI-TOF protein sequencing is a pivotal technique for determining protein amino acid sequences. This method involves combining samples with a matrix, then utilizing laser exposure to ionize proteins. These ions are analyzed by time-of-flight mass spectrometry to measure their mass-to-charge ratios. The variation in flight times provides mass data, enabling inference of amino acid composition and sequences. MALDI-TOF protein sequencing is extensively applied in proteomics for tasks such as protein id......
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Homology sequence analysis is a widely used method in molecular biology that elucidates evolutionary relationships and potential functions by comparing sequence similarities across different organisms or within the genome of a single organism. "Homologous sequences" refer to gene or protein sequences that have been inherited from a common ancestor. In molecular biology research, homology sequence analysis is a fundamental technique, crucial not only for understanding gene function but also for explori......
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Histone mass spectrometry is a sophisticated technique that utilizes contemporary mass spectrometry to investigate histones and their post-translational modifications. These histones are fundamental to chromatin architecture, influencing gene expression and chromatin dynamics through nucleosome formation with DNA. This method's high sensitivity and throughput enable precise detection of histone sequences and their modification states, offering substantial data support for studies in epigenetics, disea......
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Homology analysis is a bioinformatics approach used to elucidate evolutionary relationships and functional attributes by assessing sequence similarity across different species or genomes. In the context of molecular biology, "homology" typically denotes the resemblance between two sequences stemming from a common ancestor. There are two primary categories of homologs: orthologs and paralogs. Orthologs emerge due to speciation events, generally preserving similar functions, whereas paralogs arise from ......
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• Enzyme Purification and Characterization
Enzyme purification and characterization are crucial processes in biochemical research, involving the separation, extraction, identification, and analysis of enzymes from complex biological systems. Enzyme purification employs various biochemical techniques to eliminate other biological macromolecules and small molecular impurities, resulting in enzymes with specific activities and properties. The characterization of these enzymes entails a detailed assessment of their physical, chemical, and biologic......
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Histone analysis represents a crucial aspect of proteomics, focusing on the extensive study of histones and their post-translational modifications (PTMs). Histones, as highly conserved basic proteins, reside within the nuclei of eukaryotic cells and, in association with DNA, constitute chromatin's fundamental unit, the nucleosome. By employing histone analysis, researchers gain insights into chromatin's dynamic architecture, gene expression regulation, and cellular state transformations. PTMs of histo......
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Capillary gel electrophoresis protein analysis (CE protein analysis) involves the separation and analysis of proteins using capillary electrophoresis technology. By filling capillaries with gel media and applying an electric field, proteins are separated based on differences in charge and size. Protein molecules migrate through the capillary at varying speeds influenced by their size, shape, and charge. The gel medium acts as a sieve, allowing smaller protein molecules to pass through more easily, whi......
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• Conformational Analysis of Proteins
Conformational analysis of proteins focuses on exploring the tertiary structure and dynamic changes in proteins. Protein conformation refers to the three-dimensional spatial arrangement of atoms within a protein molecule. The tertiary structure is defined as the specific spatial form achieved when a polypeptide chain folds further based on its secondary structure. The stability of this structure is predominantly determined by non-covalent interactions such as hydrogen bonds, ionic bonds, hydrophobic i......
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• Analyzing Amino Acid Sequences
Analyzing amino acid sequences primarily aims to determine the primary structure of proteins, which is essential for understanding their functions, structural stability, evolutionary relationships, and roles in diseases. For instance, analyzing an enzyme's amino acid sequence can reveal the composition of its active site, thereby elucidating its catalytic mechanism. Amino acids, as the fundamental building blocks of proteins, serve as the foundation for this analysis. By studying their sequences, scie......
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• AUC Protein Characterization
The fundamental principle of AUC protein characterization involves systematically analyzing protein properties using ultracentrifugation techniques, with the goal of more precisely elucidating protein structure and function. This method is particularly useful for determining the dissociation constants of protein complexes, assessing molecular weight and its distribution, and evaluating shape factors. AUC protein characterization by examining their sedimentation behavior under a centrifugal force field......
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