Resources

Proteomics Databases



Metabolomics Databases

• • Principle of Differential Protein Analysis

  Differential protein analysis (DPA) is a key methodology in proteomics used to investigate changes in protein expression between different biological conditions or samples. In recent years, DPA has been widely applied in areas such as disease research, drug discovery, and biomarker identification.    Principles of Differential Protein Analysis 1. Sample Preparation and Protein Extraction DPA typically begins with a comparison of samples taken from distinct experimental groups, such as normal versus di......

• • Workflow of Proteomics Data Quality Assessment

  Proteomics is a technique used to comprehensively analyze the entire set of proteins in a cell, tissue, or organism. Proteomics datasets often contain substantial amounts of noise, redundancy, and systematic biases. If left unchecked, these issues can lead to incorrect data interpretation. The primary goal of data quality assessment is to filter out inaccurate and low-quality protein identifications and quantifications, ensuring that research findings are based on robust data.

• • Mechanism of GO Functional Annotation and Enrichment Analysis

  Gene Ontology (GO) serves as a standardized framework in bioinformatics to describe gene functions and their products. It is extensively applied in gene function annotation and enrichment analysis, offering researchers insights into the functional distributions of gene sets across biological processes, cellular components, and molecular functions, thus revealing the structure of gene regulatory networks.

• • Workflow of GO Functional Annotation and Enrichment Analysis

  Gene Ontology (GO) serves as a fundamental tool in bioinformatics for systematically describing the functions of gene products across three levels: Biological Process (BP), Cellular Component (CC), and Molecular Function (MF). GO functional annotation and enrichment analysis rely on the GO database to identify and interpret the functional trends and significance of gene sets within specific biological contexts.

• • Application of GO Functional Annotation and Enrichment Analysis

  Gene Ontology (GO) serves as a foundational tool in bioinformatics, providing researchers with a systematic framework for describing the functions of genes and their products. GO functional annotation classifies gene products into three dimensions: Biological Process (BP), Cellular Component (CC), and Molecular Function (MF), facilitating a deeper understanding of gene roles within cellular activities.

• • Principle of GO Functional Annotation and Enrichment Analysis

  Gene Ontology (GO) is a critical tool in biological research for describing the functions of genes and gene products. With the advancement of high-throughput sequencing technologies, researchers are faced with vast amounts of genetic data. GO functional annotation and enrichment analysis have become essential methods for revealing gene functions, elucidating biological processes, and predicting gene regulatory networks.

• • Advantages and Limitations of GO Functional Annotation and Enrichment Analysis

  Gene Ontology (GO) provides a standardized vocabulary for describing gene and protein functions, structured around three main domains: Biological Process (BP), Molecular Function (MF), and Cellular Component (CC). In bioinformatics analyses, GO functional annotation and enrichment analysis serve as crucial tools for understanding genomic data, enabling researchers to uncover the potential biological functions of genes and proteins.

• • Bioinformatics Interpretation of the Primary Structure of Antibody Drugs

  The primary structure of an antibody, that is, its amino acid sequence, contains a wealth of information. Bioinformatics, as an interdisciplinary field, provides us with powerful tools and techniques for interpreting the primary structure of antibody drugs. This article will focus on the application of bioinformatics in the primary structure of antibody drugs, and discuss its importance in sequence analysis, structure prediction, and function prediction.

• • Parallel Reaction Monitoring (PRM) Method in Protein Post-Translational Modificationomics

  Proteomic Post-Translational Modification (PTM) is a branch of science that studies the overall status and dynamic changes of protein post-translational modifications. It involves various types of PTM, such as phosphorylation, acetylation, SUMOylation, ubiquitination, methylation, etc. To efficiently and accurately detect and quantify the PTM status of proteins, mass spectrometry techniques, particularly targeted spectrometry methods like Parallel Reaction Monitoring (PRM), have been widely utilized.

• • Mass Spectrometry Detection of Protein Molecular Mass

  In the field of scientific research, mass spectrometry technology plays a key role in detecting the mass and structure of proteins. Compared to other protein detection methods, this technology can provide more direct and accurate results, which is why it is highly favored by researchers.   Simply put, mass spectrometry detection is the process of providing a substance with enough energy to ionize it, producing atoms or molecules with positive and negative charges.