Resources

Proteomics Databases



Metabolomics Databases

• • Applications and Strategies of SWATH-MS-Based Proteomics in Clinical Research

  In contemporary clinical research, elucidating the molecular mechanisms of diseases, identifying early diagnostic biomarkers, and guiding personalized therapeutic strategies are recognized as core scientific challenges. As a pivotal tool linking gene expression to phenotypic variation, proteomics has been extensively employed across disciplines such as oncology, immunology, and neuroscience. In recent years, SWATH-MS (Sequential Window Acquisition of All Theoretical Fragment Ion Mass Spectra), a data.......

• • Label-Free Quantitative Proteomics Service Based on SWATH Technology

  With the advancement of proteomics research, label-free quantification techniques have garnered significant attention in fields such as disease mechanism elucidation, biomarker discovery, and drug development, owing to their high throughput and elimination of complex sample pretreatment. Among these, SWATH (Sequential Windowed Acquisition of All Theoretical Fragment Ion Spectra), a Data-Independent Acquisition (DIA) strategy, has emerged as a core methodology in label-free quantitative proteomics. It.......

• • High-throughput SWATH-MS Quantitative Proteomics for Biomarker Discovery

  Biomarkers are essential for early disease screening, subtype classification, personalized therapy, and treatment monitoring. As clinical research becomes increasingly precise, the technical demands for biomarker discovery have risen, particularly in the accurate detection of low-abundance proteins and achieving cross-batch data consistency. Mass spectrometry remains a cornerstone of proteomics research. However, the Data-Dependent Acquisition (DDA) approach, due to its stochastic sampling strategy, is.....

• • What Is DIA and SWATH? Understanding Data-Independent Acquisition in Proteomics?

  Mass spectrometry is a core technology in modern proteomics research. The choice of acquisition strategy critically influences data coverage, reproducibility, and quantitative accuracy. In recent years, Data-Independent Acquisition (DIA) has rapidly gained prominence and is increasingly considered a mainstream alternative to Data-Dependent Acquisition (DDA). Within the DIA framework, the most widely recognized implementation is SWATH-MS (Sequential Window Acquisition of All Theoretical Fragment Ion ........

• • Beginner's Guide to SWATH-MS Proteomics: Principles, Advantages, and Applications

  Proteomics has become a central technology in modern life sciences for exploring cellular biology, disease mechanisms, and the discovery of biomarkers. SWATH-MS (Sequential Window Acquisition of All Theoretical Mass Spectra), a prominent technique in data-independent acquisition (DIA) strategies, is increasingly recognized as a new standard in proteomics due to its high throughput, strong reproducibility, and extensive coverage. What Is SWATH-MS? SWATH-MS is a mass spectrometry technique based on the DIA...

• • DIA/SWATH-Based Quantitative PTM Analysis

  Post-translational modifications (PTMs) play a central role in regulating cellular functions, signal transduction, metabolic processes, and the development of diseases. Modifications such as phosphorylation, acetylation, ubiquitination, and methylation confer spatiotemporal specificity and functional diversity to proteins, providing critical insights into their functional states. With advances in mass spectrometry, data-independent acquisition (DIA) techniques—particularly the SWATH-MS (Sequential .......

• • SWATH-MS Label-Free Protein Quantification Technology Analysis

  In the post-genomic era, the systematic identification and quantification of proteins has become a crucial step in elucidating biological processes and understanding disease mechanisms. Proteomics has rapidly advanced—from two-dimensional gel electrophoresis to high-resolution mass spectrometry—accompanied by the evolution of quantitative strategies toward greater accuracy and reproducibility. Among these, SWATH-MS (Sequential Window Acquisition of All Theoretical Mass Spectra), a label-free .......

• • Principle and Advantages of SWATH-MS Technology

  Proteins serve as the primary effectors of biological functions. Investigating dynamic changes in their expression profiles is crucial for elucidating biological processes, disease mechanisms, and pathways of drug action. With the continuous advancement of mass spectrometry, proteomics has evolved from a predominantly identification-driven discipline to a high-precision, quantification-centered field. In this context, SWATH-MS (Sequential Window Acquisition of All Theoretical Mass Spectra) emerged as a.....

• • Protein Separation Based on SDS-PAGE: Principles, Applications, and Advantages in Protein Research

  Protein separation and purification are critical aspects of protein research, with SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) being a widely employed technique. SDS-PAGE leverages the properties of SDS to denature proteins and impart a negative charge, facilitating the separation of proteins based on their molecular weight in an electric field. Understanding the principles and applications of SDS-PAGE is essential for elucidating protein structure and function.

• • Comprehensive Overview of Labeled and Label-Free Quantitative Proteomics Methods

  1. Labeled Quantitative Proteomics SILAC Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) is a technique for labeling proteins with stable isotopes under cell culture conditions. The SILAC experiment involves incorporating light, medium, or heavy stable isotope-labeled essential amino acids, such as lysine (Lys) and arginine (Arg), into the growth medium. As cells grow, newly synthesized proteins incorporate these labeled amino acids, thus becoming tagged with stable isotopes.