Resources
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SILAC mass spectrometry is a high-throughput quantitative analysis method based on mass spectrometry, which uses stable isotope-labeled amino acids to precisely compare protein expression levels across different samples. With the development of mass spectrometry technology, SILAC mass spectrometry has become a core technique in proteomics research, widely applied in various fields such as cancer research, drug screening, cell signaling pathway analysis, and disease mechanism studies. Through this tech......
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Peptide fragmentation is a technique that involves breaking down large macromolecular proteins into smaller peptide fragments to facilitate subsequent analysis using mass spectrometry (MS), protein identification, sequence analysis, and functional studies. Protein structure and function are pivotal in influencing the health and disease states of organisms. Thus, precise identification and analysis of protein structural features are crucial. However, due to their complex three-dimensional structures an......
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Antibody characterization refers to the in-depth analysis of the biological properties, structural features, affinity, stability, and antigen-binding capabilities of antibodies. As key immune molecules, antibodies can specifically recognize and bind to antigens, thereby performing essential immune functions such as neutralization and pathogen clearance. With the increasing application of antibody drugs and immunotherapies, the demand for antibody characterization technologies has grown significantly, ......
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• Activity Based Protein Profiling ABPP
Activity based protein profiling ABPP is a powerful technique aimed at understanding protein functions and dynamic cellular processes by specifically labeling and analyzing active proteins in biological samples. This method has broad applications in biological research, drug screening, and exploring disease mechanisms, with significant potential for identifying drug targets, revealing enzyme activities, and elucidating their roles in various diseases. ABPP enables real-time monitoring of protein activ......
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• Mass Spectrometry Based Proteomics
Mass spectrometry based proteomics enables the efficient and precise analysis of the structure, function, and interactions of thousands of proteins within biological organisms. As the most essential biomolecules in living systems, proteins execute nearly all biological functions both inside and outside the cell. The overarching goal of proteomics is to comprehensively catalog the types, quantities, and dynamic variations of all proteins present in a specific organism, cell, or tissue. By employing mas......
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SDS PAGE Gel Analysis is a widely used protein analysis technique based on the principle of separation using an electric field and polyacrylamide gel. This technique involves separating proteins in polyacrylamide gel via electrophoresis, utilizing differences in protein molecular sizes for the analysis. Sodium dodecyl sulfate (SDS), an anionic surfactant, binds to protein molecules, denaturing them by disrupting their three-dimensional structure and converting them into linear forms, while imparting a......
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Protein characterization is a crucial step in the study of protein structure, function, and its roles in biological systems. Through comprehensive protein characterization, scientists can determine important features such as amino acid sequences, three-dimensional structures, functional domains, and post-translational modifications. These insights are essential for understanding fundamental biological processes and their implications in health and disease. In fields such as biomedicine, drug developme......
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Analysis of proteomes is a scientific discipline dedicated to investigating the structure, function, and interactions of all proteins within cells, tissues, or organisms. As a critical branch of systems biology, the primary objective of proteomics is to understand the complex protein networks in organisms comprehensively. This includes revealing the variations in these networks under diverse physiological conditions and disease states, and elucidating how protein interactions and post-translational mo......
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MALDI TOF MS MS integrates the strengths of time-of-flight mass spectrometry (TOF-MS) and tandem mass spectrometry (MS/MS), offering high resolution, exceptional sensitivity, and robust capabilities for both qualitative and quantitative analyses. By sequentially guiding ionized samples through multiple stages of mass spectrometry analysis, this technology generates comprehensive and precise molecular information. It has become a critical tool in life sciences, pharmaceutical research, and environmenta......
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MALDI mass spec represents a groundbreaking advancement in mass spectrometry, widely utilized in biology, chemistry, and environmental science. Since its inception in the 1980s, it has become a vital tool for analyzing complex biological samples, particularly excelling in proteomics research due to its unparalleled performance in macromolecular analysis. The operating principle of MALDI mass spec is founded on matrix-assisted laser desorption/ionization (MALDI) technology. In this process, a sample ......
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