Resources

Proteomics Databases



Metabolomics Databases

• • The Evolution of C-Terminal Sequencing: From Traditional to Modern Techniques

  C-terminal sequencing, the methodology for determining the sequences at protein C-termini (carboxyl termini), is a critical technique in proteomics. Compared to N-terminal sequencing, the analysis of C-terminal sequences has encountered numerous challenges, making its development a reflection of both technological advancements and the evolution of methods in protein chemistry research. From traditional chemical degradation to modern high-resolution mass spectrometry, this technology has undergone sign......

• • Applications and Prospects of C-Terminal Sequencing in Proteomics

  C-terminal sequencing, a proteomics analytical method, specifically targets the identification of protein C-terminal sequences. The protein C-terminus is critical for its function, stability, degradation, and interactions. Therefore, obtaining an in-depth understanding of protein C-terminal sequences holds significant value in biological research. Recently, with continuous advancements in mass spectrometry (MS), the technology has made substantial progress and is widely applied in various proteomics f......

• • Amino Acid Analysis LC-MS Plasma

  Amino acid analysis LC-MS plasma utilizes the combined strengths of liquid chromatography and mass spectrometry for precise detection and quantification of plasma amino acids. This technique enables accurate characterization of amino acid composition, concentration, and potential modifications. As essential metabolic components, plasma amino acids play critical roles in protein synthesis, energy metabolism, and neurotransmission. Measuring plasma amino acid levels with high accuracy is crucial for ass......

• • Hybridoma Sequencing

  Hybridoma sequencing involves the genomic sequencing of cells generated through hybridoma technology, which is crucial for exploring the genomic composition of hybridoma cells. These cells are produced in laboratories by fusing antibody-producing B lymphocytes with immortalized myeloma cells, resulting in cell lines capable of both indefinite proliferation and antibody secretion. This technique underpins the production of monoclonal antibodies, essential for creating cells that retain the specificity ......

• • Glycopeptide Mapping

  Glycopeptide mapping is an analytical technique used to separate, identify, and analyze glycopeptides derived from glycoproteins following enzymatic or chemical cleavage. This method provides detailed information about glycopeptide structures, glycosylation sites, and glycan composition. Glycopeptides are macromolecules formed when sugar chains covalently bond to protein amino acid residues. Glycosylation plays a critical role in various biological processes, including cellular signal transduction, im......

• • Lipidomics Pathway Analysis

  Lipidomics pathway analysis is a method for studying the intricate processes of lipid synthesis, degradation, and transformation in living organisms. Lipids, a diverse group of organic compounds including fats, phospholipids, and sterols, are ubiquitous in biological systems. This analysis focuses on lipid molecules and their metabolic pathways, aiming to elucidate their roles and mechanisms in biological functions. By employing lipidomics pathway analysis, researchers can gain deeper insights into di......

• • Mass Spec Phosphorylation

  Mass spec phosphorylation analysis refers to the study of protein phosphorylation using advanced mass spectrometry techniques. The process begins with protein extraction from biological samples, such as cells or tissues. Due to the low relative abundance of phosphorylated proteins and the instability of phosphate groups, specialized enrichment methods, such as immunoprecipitation and chemical affinity chromatography, are commonly employed to isolate phosphorylated proteins or peptides.   Once enriched......

• • Protein-Protein Interactome

  The protein-protein interactome represents the intricate network of interactions formed by proteins both inside and outside cells. Proteins are key players in biological processes, forming the core of many life functions through their interactions. These interactions specifically influence processes such as signal transduction, metabolic pathways, cell cycle regulation, gene expression, and immune responses. Research on the protein-protein interactome not only aids in understanding the fundamental mec......

• • Absolute Protein Quantification

  Absolute protein quantification involves determining the precise amount, either in mass or molarity, of proteins within a biological sample. Unlike relative protein quantification, which compares protein levels across different samples, absolute protein quantification provides specific values for protein concentrations. This process typically combines mass spectrometry and isotope labeling techniques to facilitate precise quantification by comparing standards with samples. For instance, in cancer rese......

• • Protein Sequencing Technology

  Protein sequencing technology is fundamental to determining the amino acid sequences of proteins, providing critical insights into their structure-function relationships. This technology is widely applied in medicine, drug discovery, and biotechnology. In disease research, protein sequencing aids in identifying disease-associated protein variants, supporting early diagnosis and personalized treatment. Furthermore, sequencing specific proteins enables scientists to design engineered proteins with tailo......