Resources

Proteomics Databases



Metabolomics Databases

• • Protein Sequencing: How It Works and When Researchers Should Use It

  Protein sequencing is the process of determining amino acid sequence information from a protein or peptide sample. Depending on the question, the workflow may confirm a known sequence, recover unknown regions, identify N-terminal or C-terminal residues, measure peptide coverage, or assemble sequence evidence from LC-MS/MS data.

• • PhIP-Seq vs Protein Microarrays for Antibody Profiling

  PhIP-Seq and protein microarrays are both used for antibody profiling, but they are not interchangeable. PhIP-Seq uses a phage display peptide library and sequencing readout to identify enriched peptide targets. Protein microarrays immobilize proteins, protein fragments, or antigens on a surface and detect antibody binding by signal intensity.

• • How PhIP-Seq Works for Antibody Repertoire Profiling

  PhIP-Seq antibody repertoire profiling links antibody binding with next-generation sequencing. Antibody-containing samples are exposed to a phage display peptide library. Antibodies pull down phage clones that display recognized peptides, and sequencing reads identify which peptides were enriched.

• • PhIP-Seq Characterization of Serum Antibodies Using Oligonucleotide-Encoded Peptidomes

  Phage immunoprecipitation sequencing, widely known as PhIP-Seq, was developed for this type of discovery challenge. By combining phage-displayed peptide libraries with DNA sequencing, PhIP-Seq can characterize antibody binding patterns in serum or plasma at peptide-level resolution.

• • Identifying Disease Biomarkers with Phage Immunoprecipitation Sequencing

  Phage immunoprecipitation sequencing addresses this problem by combining phage display peptide libraries with antibody capture and next-generation sequencing. Antibody-containing samples are incubated with a library of displayed peptides.

• • Guide to PHIP-Seq: Experimental Design, Workflow, and Execution Standards

  PHIP-Seq experimental design often determines whether an antibody profiling project produces interpretable candidates or a difficult list of enriched peptides. In autoimmune disease research, infectious disease serology, vaccine response studies, and biomarker discovery, researchers may have valuable serum or plasma samples but limited tolerance for repeat experiments.

• • How Does De Novo Sequencing Work? A B2B Guide to MS/MS Logic, Use Cases, and Escalation Points

  Technical guide for How Does De Novo Sequencing Work? A B2B Guide to MS/MS Logic, Use Cases, and Escalation Points.

• • Planning Protein De Novo Sequencing for Blocked N-Termini, Sequence Variants, or Low-Reference Samples

  Technical guide for Planning Protein De Novo Sequencing for Blocked N-Termini, Sequence Variants, or Low-Reference Samples.

• • When De Novo Protein Sequencing Is the Right Choice for Unknown Proteins or Database-Mismatched Results

  Technical guide for When De Novo Protein Sequencing Is the Right Choice for Unknown Proteins or Database-Mismatched Results.

• • Peptide and Protein De Novo Sequencing by Mass Spectrometry: How Sample Type Changes the Workflow and Readout

  Technical guide for Peptide and Protein De Novo Sequencing by Mass Spectrometry: How Sample Type Changes the Workflow and Readout.