Resources
Proteomics Databases
Metabolomics Databases
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• High-Throughput Protein Sequencing
High-throughput protein sequencing is a method that utilizes advanced mass spectrometry to rapidly analyze and identify large numbers of proteins. This technique surpasses traditional protein sequencing methods by enabling the analysis of larger sample sizes within shorter time frames, thereby achieving significant advancements in both "quality" and "quantity." The essence of this technology involves the precise mass measurement of peptides by mass spectrometers, coupled with bioinformatics algorithms......
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Amino acid LC-MS analysis is a powerful analytical technique that integrates liquid chromatography (LC) and mass spectrometry (MS) to detect and quantify amino acids in complex biological matrices. As fundamental constituents of proteins, amino acids play crucial roles in diverse biological processes such as cellular growth, repair, signaling, and metabolic regulation. Thus, precise measurement of amino acid composition and concentration provides significant insights into biochemical processes, diseas......
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• Glycomics and Glycoproteomics
Glycomics and glycoproteomics are scientific disciplines focused on the study of carbohydrates within biological systems and their functional roles in cellular processes. Glycomics investigates the structures and functions of carbohydrates, emphasizing their involvement in cell communication and signal transduction pathways. In contrast, glycoproteomics delves into the glycosylation modifications on proteins, analyzing the specific sites and types of modifications and their regulatory effects on prote......
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Amino acid HPLC analysis is a critical technique for detecting and analyzing amino acid composition and content in samples. This method, widely employed in analytical chemistry, achieves compound separation through the passage of a liquid mobile phase over a solid stationary phase. Amino acids, as the fundamental building blocks of proteins in organisms, are crucial in various fields such as biomedicine, nutrition, food science, and drug development. With its high resolution, sensitivity, and reproduc......
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• Protein Extraction and Quantification
Protein extraction and quantification are essential procedures in biological science research, with widespread applications in fields such as biomedicine, drug discovery, and agricultural science. Proteins are fundamental to life, playing key roles in cell structure, metabolic regulation, and signal transduction. To gain insights into these functions, scientists extract and quantify proteins from cells or tissues. The primary goal of protein extraction is to isolate proteins from other cellular compon......
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HPLC protein quantification is a precise analytical method used to determine the concentration of proteins in samples utilizing the high resolution and sensitivity of HPLC systems. This technique is widely applicable across various fields, including biopharmaceuticals, food science, and environmental science. In biopharmaceuticals, it assists in drug development and quality control by ensuring protein content in pharmaceuticals complies with standards. In food science, it facilitates the detection of ......
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• C-Terminal Sequencing: 8 Critical Mistakes to Avoid in LC-MS/MS Analysis
LC-MS/MS analysis is extensively applied in proteomics for protein identification, post-translational modification profiling, and quantitative studies. However, during C-terminal sequencing, researchers often make avoidable mistakes in experimental design, data acquisition, and data analysis, which can compromise data quality and the reliability of conclusions. This article highlights eight critical mistakes commonly encountered in LC-MS/MS-based C-terminal sequencing, and provides practical strategie......
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• How to Improve C-Terminal Sequencing Sensitivity and Accuracy with LC-MS/MS?
In C-terminal sequencing with LC-MS/MS, improving sensitivity and accuracy requires a systematic and integrated approach involving sample preparation, optimization of instrumental settings, and data analysis strategies. The following are major strategies for enhancement: Selection of Appropriate Proteases and Sample Preparation Strategies The key to successful C-terminal sequencing lies in achieving high specificity and efficiency in generating C-terminal peptides. While conventional proteases such ......
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• LC-MS/MS Based C-Terminal Sequencing: Technical Advances and Challenges
One of the central objectives of proteomics is to elucidate the complete amino acid sequences of proteins. In this context, C-terminal sequencing has attracted increasing attention in recent years due to its critical role in understanding protein modifications, degradation pathways, and biological functions. The rapid advancement of liquid chromatography–tandem mass spectrometry (LC-MS/MS) has offered powerful analytical capabilities for C-terminal sequencing. However, challenges remain due to the lim......
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BiFC analysis is a technique used to investigate protein-protein interactions. This method leverages the reconstitution of fluorescent protein fragments, where a fluorescent protein (e.g., yellow fluorescent protein (YFP) or enhanced green fluorescent protein (EGFP)) is divided into two non-fluorescent fragments, each fused to a different protein of interest. When these proteins interact within cells or in vitro, the protein fragments come into proximity and reassemble, restoring the fluorescence sign......
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