Resources

Proteomics Databases



Metabolomics Databases

• • N-Terminal Sequencing Method

  The N-Terminal sequencing method is primarily employed to ascertain the amino acid sequence at the N-terminus of proteins or peptides. This method encompasses techniques such as Edman degradation and mass spectrometry analysis. First introduced in the 1950s, the Edman degradation technique determines sequences by systematically removing and identifying the N-terminal amino acids of a peptide chain. Noted for its high specificity and accuracy, it is particularly effective for analyzing short-chain pept......

• • High-Throughput Protein-Protein Interaction

  High-throughput protein-protein interaction refers to the process of efficiently and automatically detecting and analyzing numerous protein interactions using advanced experimental techniques. Proteins are fundamental molecules performing nearly all functions in organisms, and their interactions are crucial for cellular functions, signal transduction, metabolic pathways, and gene regulation. Understanding protein interaction networks is essential for elucidating cellular mechanisms and their alteratio......

• • In Vitro Protein-Protein Interaction Assay

  In vitro protein-protein interaction assays are essential for investigating the mechanisms of protein recognition and binding. These assays form the basis for understanding complex biomolecular networks, which is crucial for elucidating intracellular signal transduction, gene regulation, and metabolic pathways. By employing in vitro techniques, scientists can reconstruct functional network maps of cellular processes, enhancing our understanding of disease mechanisms and facilitating the identification......

• • How to Obtain Comprehensive Protein Information Through de Novo Sequencing

  The goal of de novo sequencing is to analyze the complete amino acid sequence of proteins from mass spectrometry data without bias, including information about mutations, post-translational modifications (PTMs), and isoforms. In order to obtain a comprehensive understanding of protein information, strategies such as optimizing sample preparation, using advanced mass spectrometry techniques, applying multiple fragmentation modes, utilizing intelligent data analysis, and performing PTM analysis must be ......

• • N-Terminal Sequencing: A Comparative Analysis of Edman Degradation and Mass Spectrometry Approaches

  N-terminal sequencing is a widely employed technique for determining the amino acid sequence of proteins. It plays a crucial role in proteomics, molecular biology, and biopharmaceutical research by elucidating protein structural characteristics, modifications, and post-translational processing. The primary methods used for N-terminal sequencing include Edman degradation, a classical chemical approach, and mass spectrometry, a modern analytical technique. These two methods differ markedly in their unde......

• • Measuring Protein-Protein Interactions

  Measuring protein-protein interactions is a crucial technique in biochemistry and molecular biology, used to explore how proteins interact and to elucidate their roles within the cell. This approach enhances our understanding of cellular signal transduction, metabolic pathways, gene expression regulation, and the molecular mechanisms underlying various diseases. As proteins are essential for life processes, they often require interactions with other proteins or biomolecules to function optimally. Such......

• • Studying Protein-Protein Interactions

  Studying protein-protein interactions is a critical research area in both biology and medicine, aimed at understanding the mechanisms of interactions between proteins and their roles in various biological processes. As fundamental biomolecules, proteins perform diverse functions within and outside cells. However, individual proteins often cannot achieve their biological functions independently. Therefore, studying protein-protein interactions is essential for gaining insights into the complex molecula......

• • PTM Analysis

  PTM analysis refers to studying various types, sites, extents, and functional impacts of these modifications. Once proteins are synthesized, PTMs can alter their structure and function by adding chemical groups, cleaving, or modifying the protein backbone. This regulates protein activity, localization, stability, and interactions with other molecules. For instance, phosphorylation may activate or inhibit enzymatic activity, while ubiquitination generally relates to protein degradation. PTM analysis pr......

• • Fluorescence Polarization Protein-Protein Interaction

  Fluorescence polarization protein-protein interaction is a widely used technique for studying protein interactions by detecting changes in fluorescence polarization. When plane-polarized light of a specific wavelength excites a fluorescent molecule, the emitted fluorescence retains a degree of polarization, which is influenced by the rotational mobility of the molecule. Larger molecular complexes exhibit slower rotational motion and higher fluorescence polarization, while smaller molecules rotate fast......

• • Co-Immunoprecipitation Analysis

  Co-immunoprecipitation analysis is a robust experimental technique employed to investigate protein-protein interactions. This methodology enables researchers to selectively isolate and enrich specific protein complexes from complex biological samples. Fundamentally, co-immunoprecipitation analysis leverages specific antibodies for the recognition and binding of target proteins, which are subsequently separated from the solution along with their interacting partners using a solid-phase carrier, such as......