Resources
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Circular Dichroism Analysis is a commonly used technique, widely applied in the field of biological products and disease research. Principle of Circular Dichroism Analysis Circular Dichroism Analysis is an analytical method to study the structure and properties of molecules by measuring their absorption and rotation of circularly polarized light. Circular Dichroism takes advantage of the optical rotation effect of chiral molecules on circularly polarized light for its measurements, and provides imp......
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• What are the Prospects for Protein Quantification in Biological Research?
Protein quantification is significant for understanding biological processes, disease mechanisms, and drug development, providing us with accurate methods to measure protein content, and has broad application prospects in biological research. Principles of Protein Quantification The principle of protein quantification is based on the chemical reaction or physical interaction between proteins and specific reagents, which produces measurable signals or changes. Common protein quantification methods in......
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• Protein Molecular Weight Analysis by SDS-PAGE: Method Comparison and Best Practices
SDS-PAGE (Polyacrylamide Gel Electrophoresis) is a widely used method for determining protein molecular weight, based on the relationship between the migration rate of proteins in an electric field and their molecular weight. Technical Principles of the SDS-PAGE Method The SDS-PAGE technique utilizes sodium dodecyl sulfate (SDS) to denature and unfold proteins, imparting a uniform negative charge. Under the influence of an electric field, the proteins migrate through a polyacrylamide gel. The relati......
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Circular dichroism technology is an analytical technique that studies the structure of chiral molecules by measuring their absorption and rotation of circularly polarized light. Chiral molecules are composed of molecules with a chiral center, exhibiting optical rotation. Circular dichroism technology obtains structural information of chiral molecules by measuring the difference in absorption of left-handed and right-handed circularly polarized light in samples. Importance of Circular Dichroism Techn......
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Traditional research methods have certain limitations in the detection and analysis of small molecule peptides. However, with the development of metabolomics and proteomics technologies, we can use these efficient and accurate methods to reveal the functions of small molecule peptides and the mechanisms of related diseases. Principles and Applications of Metabolomics 1. Principles of Metabolomics Metabolomics is the exploration of metabolic pathways and the relationships between metabolites by analy......
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• Protein Interaction Network Analysis and Interpretation Based on Pull-Down Mass Spectrometry
Pull-Down Mass Spectrometry (PDMS) technology serves as an effective method for analyzing protein interactions, enabling researchers to uncover protein interaction networks. This provides crucial insights for understanding signaling pathways and elucidating disease mechanisms. Principles of Pull-Down Mass Spectrometry Pull-Down Mass Spectrometry is a protein interaction analysis technique based on affinity chromatography. The fundamental principle involves using specific antibodies or affinity agent......
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• Exploring the Application Potential of Pull-Down Assays for Disease-Related Protein Identification
Pull-down assays, a widely used method for analyzing protein-protein interactions, have shown significant potential in disease-related research. Principle and Procedure of Pull-Down Assays Pull-down assays are experimental techniques designed to identify and characterize protein-protein interactions. These assays typically involve the following steps: 1. Antibody Immobilization Specific antibodies are covalently bound to a solid-phase matrix, such as agarose or magnetic beads. 2. Sample Incubati......
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• Protein Pull-Down: A Powerful Tool for Investigating Protein-Protein Interactions
Protein Pull-Down is a highly effective method for capturing target proteins and identifying their interacting partners using specific affinity agents. This technique leverages the specificity of protein-protein interactions to co-capture target proteins alongside their interacting partners through well-defined binding reactions. Technical Principles and Workflow of the Protein Pull-Down Technique The Protein Pull-Down technique comprises four key steps: sample preparation, affinity agent binding, w......
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Peptide fragmentation is a technique that involves breaking down large macromolecular proteins into smaller peptide fragments to facilitate subsequent analysis using mass spectrometry (MS), protein identification, sequence analysis, and functional studies. Protein structure and function are pivotal in influencing the health and disease states of organisms. Thus, precise identification and analysis of protein structural features are crucial. However, due to their complex three-dimensional structures an......
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SILAC mass spectrometry is a high-throughput quantitative analysis method based on mass spectrometry, which uses stable isotope-labeled amino acids to precisely compare protein expression levels across different samples. With the development of mass spectrometry technology, SILAC mass spectrometry has become a core technique in proteomics research, widely applied in various fields such as cancer research, drug screening, cell signaling pathway analysis, and disease mechanism studies. Through this tech......
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