Resources

Proteomics Databases



Metabolomics Databases

• • Methylation Analysis

  Methylation analysis is an experimental method used for detection and study of DNA, RNA, and protein methylation. Protein methylation involves adding a methyl group to specific amino acid residues in a protein, typically lysine or arginine. This modification has significant effects on protein function, interactions, and localization. Below are some commonly used techniques for protein methylation analysis:

• • Protein TMT

  TMT (Tandem Mass Tag) is a commonly used mass spectrometry quantification technique in proteomics. It labels proteins or peptides in different samples using a series of chemical tags, then detects and compares them in mass spectrometry analysis. This method allows for the simultaneous analysis of protein expression differences in multiple samples, thus being used to compare protein expression levels under different conditions.

• • What is the Control Group for Protein Differential Analysis

  In protein differential analysis, the control group refers to the sample or condition used as a reference in experimental design for comparison with the experimental group. The setting of the control group is crucial for ensuring the validity and reliability of the experimental results.   In protein differential analysis, the specific nature of the control group depends on the purpose and design of the research.

• • Antibody Glycosylation Detection

  Antibody glycosylation detection is a technology used to analyze the glycan structure and composition on antibody molecules. Glycosylation is a common post-translational modification process of proteins, which is crucial to the function of antibodies, including their stability, recognition, and antigen binding ability. In the biopharmaceutical industry, especially in the process of developing and producing monoclonal antibody drugs

• • DIA Proten Omics

  Data-Independent Acquisition Proteomics (DIA) is an omics technology used to systematically analyze the proteins in biological samples. Compared with traditional Data-Dependent Acquisition (DDA) methods, DIA technology can analyze more proteins and peptides at the same time, improving the comprehensiveness and repeatability of detection.

• • Detection of Protein Acetylation

  Protein acetylation is an important post-translational modification which typically involves the transfer of an acetyl group from acetyl coenzyme-A (Acetyl-CoA) to the lysine residues of a protein. This modification is crucial for the regulation of protein function, localization, and interaction, particularly playing a central role in the regulation of gene expression.

• • Protein Detection for Ubiquitination

  Ubiquitinated protein detection is to identify and quantify the status of proteins after ubiquitination post-translation modification. Here are some commonly used methods to detect ubiquitinated proteins:   1. Western Blot Analysis Western blotting is a common method for detecting ubiquitinated proteins. This process usually involves: (1) Extracting protein samples. (2) Separating proteins using SDS-PAGE electrophoresis. (3) Transferring proteins onto a membrane.

• • Thermal Stability Analysis of Recombinant Protein Vaccines

  Recombinant protein vaccines are a type of vaccine that do not contain complete pathogens and are prepared by specific protein antigens produced by heterologous expression systems. The common heterologous expression systems include bacteria, mammalian cells, plant cells, and insect cells. The appropriate heterologous expression system needs to be selected according to the type of antigen produced.

• • Protein Quantification Assay

  Protein quantification is a common technique in experimental biology, used to measure the concentration or total amount of protein in a sample. This measurement is very important for biochemistry, molecular biology, medical research, and other fields.   Analysis Workflow 1. Sample Preparation Cell extract, tissue samples, serum, plasma, urine or other biological samples containing protein. Ensure the sample is clean and free of impurities.

• • Parallel Reaction Monitoring (PRM) Detection of Ubiquitination

  Parallel Reaction Monitoring (PRM) is a high-resolution mass spectrometry analysis technique commonly used in targeted proteomics research. It can be used to detect and quantify specific proteins and protein modifications, such as ubiquitination.