Resources

Proteomics Databases



Metabolomics Databases

• • Struggling with N-Terminal Protein Sequencing? 2 Common Issues and How to Fix Them!

  In proteomics, N-terminal protein sequencing plays a critical role in elucidating the initiation sites of proteins, post-translational modifications (PTMs), signal peptide cleavage, and protein degradation pathways. However, a frequent technical challenge encountered by researchers is the inability to detect the N-terminal protein using standard methods. This article explores the mechanistic basis of two major technical bottlenecks in N-terminal protein sequencing and presents practical solutions to .......

• • Edman Sequencing vs. Mass Spectrometry: A Comparison of Protein Sequencing Techniques II

  Proteins, as central functional molecules within cells, carry amino acid sequence information that not only elucidates their structure and function but also underpins various applications such as biomarker discovery, antibody characterization, and vaccine development. As a fundamental technique for deciphering amino acid composition and sequence, protein sequencing has evolved over several decades into two dominant methodologies: Edman sequencing and Mass Spectrometry (MS). What are the unique advantages...

• • Proteomics in Biotechnology

  Proteomics in biotechnology is a discipline that focuses on the comprehensive study of the proteome—the complete set of proteins expressed by an organism—encompassing their structures, functions, dynamic alterations, and interactions. Proteins are fundamental to life, executing essential cellular functions such as catalyzing metabolic reactions, DNA replication, and signal transduction. The advent of proteomics enables researchers to systematically explore the intricate network of proteins and their r......

• • APEX Proximity Labeling

  APEX proximity labeling, utilizing engineered ascorbate peroxidase (APEX), is a robust method for investigating protein interactions and subcellular localization within living cells. The central component of this technique is the modified peroxidase APEX, which is fused to target proteins or cellular structures to facilitate rapid and covalent labeling of proximal proteins. When compared to traditional approaches like immunoprecipitation and yeast two-hybrid methods, APEX proximity labeling offers enh......

• • Websites for Analyzing Glycosylation Sites

  Various websites for analyzing glycosylation sites have been developed to support the accurate prediction of glycosylation patterns in proteins. These platforms offer computational tools for identifying potential N-, O-, and C-linked glycosylation, as well as non-enzymatic glycation and GPI anchoring. Below are some widely used and well-documented resources that enable researchers to streamline their glycosylation analysis. NetNGlyc & NetOGlyc 1. Website: NetNGlyc and NetOGlyc 2. Function: These tools are..

• • Western Blot Detection of Histone Methylation

  Western blot detection of histone methylation is a crucial approach in epigenetics, enabling researchers to investigate how histone modifications regulate chromatin structure and transcriptional activity. Histone methylation is a form of post-translational modification that critically influences gene expression programs. Catalyzed by histone methyltransferases (HMTs), this process involves the enzymatic transfer of methyl groups from the donor S-adenosylmethionine to lysine residues on histone tails.

• • Amino Acid Full Sequence Analysis

  Amino acid full sequence analysis serves as a foundational approach for understanding protein function, beginning with the elucidation of the primary structure—the linear arrangement of amino acids connected by peptide bonds to form a polypeptide chain. This initial level of analysis provides critical insights into the molecular architecture and biological roles of proteins and typically involves the following steps: 1. Determination of the Amino Acid Sequence The amino acid sequence is established using...

• • Microtubule Protein Acetylation Detection

  Microtubule protein acetylation detection is a key analytical approach used to assess the post-translational modification status of tubulin proteins, which are critical structural components of the cytoskeleton. Microtubules are primarily composed of α- and β-tubulin heterodimers and play essential roles in various cellular processes. 1. Maintenance of Cell Morphology Microtubules provide essential structural support that helps preserve cellular shape and mechanical stability. 2. Intracellular Transport....

• • Commonly Used Proteases in Protein Sequencing

  Protein sequencing refers to the process of determining the amino acid sequence of a protein. Commonly used proteases in protein sequencing are essential to this process, as they enzymatically cleave proteins into smaller peptide fragments suitable for analysis. Below are commonly used proteases and their detailed characteristics: Trypsin 1. Cleavage Specificity Trypsin cleaves peptides at the carboxyl side of lysine (K) and arginine (R) residues. 2. Key Features As the most widely used protease in ........

• • Methods of Proteolytic Digestion in Proteomics

  Proteolytic digestion plays a pivotal role in proteomics by breaking down proteins into smaller peptide fragments, enabling comprehensive downstream analysis and accurate protein identification. This procedure is essential for elucidating protein structure and function, as well as understanding their involvement in various biological processes. The following is a detailed overview of commonly used methods for proteolytic digestion: Preparation Work 1. Protein Sample Preparation The initial step involves....