Resources
Proteomics Databases
Metabolomics Databases
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• Proximity Labeling Proteomics
Proximity labeling proteomics is an advanced biochemical approach designed to map protein spatial relationships and dynamic interactions within cells. By genetically fusing specific enzymes such as biotin ligase BirA or peroxidase APEX to target proteins, these enzymes can selectively label proximal proteins with biotin tags. The biotinylated proteins are subsequently enriched via streptavidin affinity purification and analyzed using high-resolution mass spectrometry for identification and quantificat......
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mRNA analysis is a method for investigating messenger RNA (mRNA) molecules, which serve as essential intermediaries in gene expression by conveying genetic instructions from DNA to the protein synthesis machinery. This technique primarily aims to determine gene expression levels and patterns, providing insights into the regulation of genes under various conditions. By examining mRNA expression, researchers can pinpoint disease-related genes, identify critical regulatory nodes in biological pathways, a......
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• Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry
Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) is a powerful analytical technique extensively used in proteomics, chemistry, drug development, and clinical diagnostics. Its principle relies on the absorption of laser energy by matrix molecules, enabling the desorption and ionization of sample molecules. The flight time of these ions is then measured by a time-of-flight mass spectrometer to determine their mass. The efficiency of this technique largely depen......
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Enzyme Identification refers to the identification and characterization of enzymes in biological samples through a series of experimental and analytical approaches. Enzymes are proteins that serve as catalysts for biochemical reactions, playing critical roles in cellular metabolism, signal transduction, and gene expression regulation. Understanding enzyme types and functions is essential for elucidating biological mechanisms, developing novel therapeutics, and advancing biotechnological applications. ......
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Protein domain analysis is an essential component of protein research. Protein domains are distinct structural and functional units within protein macromolecules, capable of existing in a stable state and performing specific functions independently. In the drug development field, protein domain analysis is widely applied in the screening and design of drug targets. By analyzing protein domains, researchers can elucidate the binding mechanisms between drug molecules and protein targets, facilitating th......
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Protein expression analysis is an analytical technique used to investigate the levels and dynamic changes of proteins within organisms. By examining the expression levels and states of proteins in cells or tissues, this technique provides insights into their functional roles in biological processes. Through protein expression analysis, researchers can elucidate the molecular mechanisms underlying various biological phenomena, such as cell differentiation, signal transduction, and immune responses. In ......
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• A Powerful Tool for Small-Molecule Drug Target Research: Thermal Proteomic Profiling (TPP)
Thermal Proteomic Profiling (TPP) is a high-throughput and unbiased proteomic screening method that integrates thermal stability analysis with mass spectrometry. The core principle of TPP is that drug-protein interactions influence protein thermal stability, leading to increased stability of drug-bound proteins upon heating. Monitoring changes in protein thermal stability provides valuable insights into ligand binding and represents a crucial step in drug target discovery. When cells or cell lysates a......
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MS/MS sequencing is a powerful technique for determining amino acid sequences in complex protein mixtures. This method employs two stages of tandem mass analyzers to analyze peptides. Proteins are first digested into smaller peptide fragments, and a specific peptide is selected by the first mass analyzer. The peptide is then fragmented further in a collision cell, with the second mass analyzer measuring the mass-to-charge ratios of the resulting fragments. By interpreting this fragment data, the amino......
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• N-Terminal Sequencing Analysis
N-terminal sequencing analysis is a pivotal technique in proteomics used to determine the N-terminal amino acid sequence of proteins or peptides. This sequence provides critical insights into the biochemical properties, biological functions, and evolutionary relationships of proteins, which are fundamental to life processes. In the biopharmaceutical industry, N-terminal sequencing analysis plays an integral role in drug development and quality assurance. It verifies the N-terminal sequence of recombin......
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LC-MS/MS protein sequencing is a powerful analytical technique for identifying and characterizing proteins in complex samples. This method begins with liquid chromatography to separate protein mixtures into simpler components based on physical and chemical properties such as molecular weight and hydrophilicity. The separated proteins are then ionized and analyzed using tandem mass spectrometry, which measures the mass-to-charge ratios of fragmented peptides, providing detailed sequence information. ......
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