Resources

Proteomics Databases



Metabolomics Databases

• • Capillary Isoelectric Focusing (CIEF) Analysis of Peptide Drugs

  Peptide drugs are bioactive molecules composed of multiple amino acids connected by peptide bonds. Typically, they are made up of 10~100 amino acids, with a relative molecular mass less than 10,000. Most peptide drugs are derived from endogenous peptides or natural peptides, so they have little or no side effects on the human body. Compared with protein drugs, peptide drugs also have advantages such as good stability, high purity, low production cost, and low immunogenicity.

• • Q-TOF Peptide Map Analysis Steps

  The Q-TOF (Quadrupole-Time of Flight) mass spectrometer is a mass spectrometry device that combines quadrupole and Time of Flight (TOF) technologies. It combines the selective ion transmission of quadrupole mass spectrometry and the high resolution, high precision measurement capabilities of Time of Flight mass spectrometry. It is often used for peptide mass spectrometry analysis, especially in protein identification and proteomics research.

• • De Novo Sequencing of Nasopharyngeal Carcinoma Tissue

  Nasopharyngeal carcinoma, a malignant tumor located in the head and neck, has a complex pathogenesis closely related to factors such as genetics, environment, and viral infections. Due to its hidden location and diverse clinical manifestations, it brings certain challenges to its early diagnosis and the choice of treatment plan. In this context, it is particularly important to perform de novo sequencing of nasopharyngeal carcinoma tissues.

• • Tripeptide Sequences: The Biological Code of the Microcosm

  In the broad field of biology, proteins are renowned for their unique and diverse functions. The driving forces behind these functions are often short amino acid sequences in proteins, such as tripeptides. These small fragments consisting of three amino acids, though small, play a key role in processes such as signal transduction, protein interactions, and metabolic regulation.

• • De Novo Sequencing: Unveiling Unknown Peptides

  "De novo" sequencing is a technique in bioinformatics mainly used to determine the amino acid sequences of new or unknown proteins or peptides. Compared to re-sequencing, this technique pays special attention to those proteins or peptides that have not been identified or are not present in existing databases.

• • Secretory Protein Sequencing: Decoding Extracellular Proteins

  Secretory proteins are those proteins that are released to the outside of the cell through the cell's secretion pathway. They play a key role in various biological processes, such as immune responses, cell signaling, inflammatory responses, etc. The sequencing of secretory proteins is to determine their amino acid sequence, thereby understanding their function, structure, and interactions with other proteins or small molecules.

• • O-Glycosylation and Modification Site Analysis in Bone Tissue

  Bone tissue, as the hardest tissue in the human body, plays important roles in supporting body weight and protecting internal organs. However, the health and stability of bone tissue are not only dependent on its mineralization degree and density, but also on the complex interactions of various biomolecules in bone cells. Among them, protein glycosylation is a key process.

• • Mechanism of Pull-Down Coupled with MS for Protein Identification

  Protein pull-down combined with mass spectrometry (MS) is a pivotal technique for investigating protein-protein interactions. This method allows for the specific capture of a target protein and its interacting partners, followed by MS analysis, ultimately identifying the group of proteins that directly or indirectly interact with the target protein. Below is a detailed explanation of the mechanisms underlying protein pull-down and MS identification.

• • Application of Pull-Down Coupled with MS for Protein Identification

  Proteins play critical roles in living organisms, executing various functions in cellular signaling, metabolic regulation, and gene expression. Protein-protein interactions are fundamental to these processes and are closely tied to many diseases' onset and progression. Studying these interactions is therefore vital for understanding biological processes and developing innovative therapies.

• • Workflow of Pull-Down Coupled with MS for Protein Identification

  Protein pull-down assays combined with mass spectrometry (MS) analysis are powerful techniques for investigating protein-protein interactions. By purifying and identifying proteins that interact with a target protein, this method offers invaluable insights into the functions of proteins and the biological processes they participate in. Below is a detailed description of the workflow involved in protein pull-down and mass spectrometry identification.