Resources

Proteomics Databases



Metabolomics Databases

• • Protein Sequencing by Edman Degradation

  Protein Sequencing by Edman Degradation is a well-established method for determining the amino acid sequences of proteins. Developed in 1950 by the Swedish biochemist Pehr Edman, this technique enables stepwise sequencing by cleaving and labeling the N-terminal amino acids of a protein. Specifically, phenyl isothiocyanate (PITC) reacts with the N-terminal amino acid to form a phenylthiohydantoin derivative, which is selectively cleaved under acidic conditions to release a cyclic compound. This derivat......

• • Peptide Characterization

  Peptide characterization is a fundamental aspect of proteomics research, focusing on the comprehensive analysis of peptide structures, sequences, properties, and functions. Peptides, which are small molecular fragments typically composed of 2 to 50 amino acids linked by peptide bonds, are derived from protein degradation. Despite their simpler structures compared to intact proteins, peptides exhibit diverse and complex biological functions, including roles in signal transduction, immune regulation, an......

• • HCP Coverage Analysis

  HCP coverage analysis is a key technique for detecting and quantifying residual host cell proteins (HCPs) in biopharmaceutical products. During the production of therapeutic proteins, host cells such as E. coli, yeast, or mammalian cells are commonly used. However, these cells may release or secrete various non-target proteins, collectively known as HCPs, during production. The presence of HCPs can compromise drug safety and efficacy, necessitating accurate detection and analysis throughout biopharmac......

• • HPLC Method for Protein Analysis

  HPLC method for protein analysis is a robust and versatile technique used to separate protein components in a sample by employing a liquid mobile phase that flows through a chromatography column. This approach facilitates the fractionation of proteins based on specific properties such as size, hydrophobicity, and charge. Compared to traditional analytical methods, HPLC method for protein analysis offers significant advantages, including faster analysis and improved detection precision and accuracy. Re......

• • Aptamer-Based Proteomics

  Aptamer-based proteomics involves the analysis of protein composition and abundance through the specific recognition and binding capabilities of nucleic acid aptamers. These aptamers, composed of DNA or RNA sequences, are identified using SELEX (Systematic Evolution of Ligands by EXponential enrichment)—an in vitro selection process that isolates nucleic acid sequences with high affinity and specificity for target proteins from a vast library.   Compared to traditional antibody-based detection methods......

• • Edman Degradation Protein Sequencing

  Edman degradation protein sequencing is a classical technique for protein sequence analysis that facilitates the precise determination of protein amino acid sequences through sequential removal of amino acid residues. Developed in the 1950s by Swedish chemist Pehr Edman, this method has become an indispensable tool in proteomics research. The core principle of Edman degradation involves the selective binding of specific chemical reagents to the amino terminus of a protein, followed by stepwise removal......

• • SEC-MALS Analysis

  SEC-MALS analysis is an advanced technique that combines Size Exclusion Chromatography (SEC) and Multi-Angle Light Scattering (MALS) to determine the absolute molecular weight, molecular size, and aggregation state of biomolecules. This method is widely employed in fields such as structural biology, proteomics, and biopharmaceuticals. The principle of SEC-MALS analysis involves separating sample components based on molecular size using SEC, followed by real-time analysis of the eluted fractions via an......

• • Proteomics Identify Differential Proteins

  Proteomics is a scientific approach focused on studying the protein composition and functionality within biological systems. Differential protein analysis is a key method for assessing variations in protein expression levels under different experimental conditions, such as comparing control and experimental groups. The following are standard methodologies for differential protein identification:

• • Protein Identification by Small Molecule Mass Spectrometry

  Protein small molecule identification using mass spectrometry is a technique employed to analyze and determine the composition and structure of proteins or peptides. This method primarily operates by measuring molecular mass and the mass-to-charge ratio (m/z), constituting a pivotal technology in proteomics research. The fundamental steps involved are as follows:

• • Quantitative Detection of Different Proteins Within a Cell

  Quantitative detection of different proteins within cells is a crucial aspect of biological and biomedical research. It aims to understand changes in protein expression levels, protein networks, and their roles in cell function and disease.