Resources

• • What are the Methods of Protein Sequencing?

  The methods of protein sequencing are different techniques for revealing the amino acid sequence of proteins. Protein sequencing provides a direct means to determine the amino acid sequence of proteins, forming a cornerstone for studying protein structure and function. Sequencing facilitates the prediction of three-dimensional protein structures, offering insights into their biological functions and mechanisms of interaction.   Currently, the primary methods of protein sequencing include mass spectrom......

• • What are the Uses of Mass Spectrometry in Protein Analysis?

  Mass spectrometry in protein analysis is a powerful analytical tool that has become indispensable in numerous scientific fields, enabling researchers to examine the composition, structure, and functions of biomolecules with remarkable detail. Protein analysis, crucial for understanding biological processes, relies on mass spectrometry in protein analysis to reveal protein structures, functions, interactions, and regulatory mechanisms, which are foundational to advancements in disease diagnosis, drug d......

• • What is the Mass Spectrometry Technique for Proteins?

  Mass spectrometry technique for proteins is a sophisticated analytical technique used for the high-throughput identification and quantification of proteins. This approach involves ionizing protein or peptide samples, separating the resulting ions by their mass-to-charge ratios in a mass spectrometer, and then detecting them. By analyzing these ions, researchers can infer protein structure, functional characteristics, and interaction networks. Protein mass spectrometry has become invaluable in biomedic......

• • Why is Protein Sequencing Better than DNA Sequencing?

  Protein sequencing and DNA sequencing are two distinct molecular analysis techniques. DNA sequencing involves the analysis of deoxyribonucleic acid (DNA), the primary carrier of genetic information, which is located in the cell's nucleus. In contrast, protein sequencing focuses on proteins, which are the principal molecules performing diverse functions within the cell. Proteins are encoded by DNA and synthesized through RNA transcription and translation.   Common methods for DNA sequencing include San......

• • Mass Spectrometry-Based De Novo Protein Sequencing

  De novo protein sequencing refers to the process of determining the amino acid sequence of peptides using tandem mass spectrometry (MS/MS) without relying on pre-existing sequence databases.   The core principle of de novo protein sequencing is to calculate the mass of amino acid residues in the peptide backbone by measuring the mass difference between fragment ions. The mass-to-charge ratio (m/z) of these ions typically allows for the unique identification of the residue type, thereby determining the......

• • How is the Protein Sequence Obtained?

  Proteins are essential drivers of life processes, performing a wide array of functions, from catalyzing biochemical reactions to constructing cellular structures. Understanding protein structure and function is crucial in fields such as biology, medicine, and biotechnology. The protein sequence, which refers to the amino acid sequence, is a fundamental aspect of understanding both protein function and structure. This article outlines several methods for obtaining protein sequences.   Database Search W......

• • What is the Protein Sequence?

  A protein sequence, defined as the arrangement of amino acids in a protein molecule, constitutes the primary structure of the protein, also known as its basic structure. This sequence not only determines the protein's three-dimensional conformation but also influences its biological function within the organism.   Protein Sequence—Types of Amino Acids Proteins are macromolecules made up of 20 standard amino acids linked by peptide bonds. These amino acids include glycine (Gly), alanine (Ala), valine (......

• • What are the Main Functions of Olink Proteomics?

  Olink proteomics is an advanced biotechnology that enables high-throughput, high-sensitivity, and high-specificity quantitative analysis of specific proteins in biological samples. This is achieved using the innovative Proximity Extension Assay (PEA) technology.   Core Technology and Advantages The central technology behind Olink proteomics is the PEA technique, which involves the specific recognition and binding of antibodies to target proteins, followed by the amplification of oligonucleotide se......

• • What is the Role of Protein Sequencing?

  Protein sequencing, as a key method for deciphering protein structure and function, plays an indispensable role in these processes. In 1954, Sanger successfully sequenced bovine insulin, confirming for the first time the role of amino acids in protein synthesis, thus marking the beginning of the protein sequencing field. In the early 1970s, Sanger developed DNA sequencing technology, laying the foundation for the modification of protein sequences. Proteins are critical macromolecular organic compound......

• • What are the Steps of Edman Degradation?

  Edman degradation involves a series of steps to determine the amino acid sequence of a protein or peptide, specifically targeting the N-terminal amino acid. These steps include: 1. Adsorption of the Sample The protein or peptide to be sequenced is adsorbed onto a solid surface, with glass fiber coated with polybutadiene (a cationic polymer) being a commonly used support material. This is the first of the steps of Edman degradation where the sample is prepared for further chemical reactions.   2. Coupl......