Resources

Proteomics Databases



Metabolomics Databases

• • Application of SILAC-Based Co-IP-MS in Protein Interaction Networks

  Protein-protein interactions are central to numerous biological processes within cells. Understanding these interactions is critical for elucidating cellular functions, signal transduction, and disease mechanisms. The Co-IP-MS technique based on SILAC (Stable Isotope Labeling by Amino acids in Cell culture) offers a powerful tool for studying the dynamic changes in protein-protein interactions.

• • Workflow of SILAC-Based Co-IP-MS for Protein Interaction Analysis

  Protein-protein interactions are fundamental to biological processes within cells. Understanding these interactions is crucial for unraveling complex signaling pathways and molecular mechanisms. In proteomics research, the combination of SILAC (Stable Isotope Labeling by Amino acids in Cell culture), immunoprecipitation (Co-IP), and mass spectrometry (MS) has become a pivotal method for analyzing protein-protein interactions.

• • Advantages and Disadvantages of SILAC-Based Co-IP-MS in Protein Interaction Analysis

  Protein-protein interactions are crucial in many biological processes and signaling pathways within cells. With advancements in proteomics technologies, SILAC-based Co-IP-MS has become a key tool for studying these interactions. This method combines stable isotope labeling, immunoprecipitation, and mass spectrometry, offering researchers an efficient and precise means to capture and quantitatively analyze the complex dynamics of protein-protein interactions.

• • Application of De Novo Antibody Sequencing

  Antibody de novo sequencing is an innovative technology designed to determine the amino acid sequence of unknown antibodies. Compared to traditional sequencing methods, de novo sequencing offers enhanced accuracy and completeness. This technique has broad application prospects in biomedical research, drug development, and diagnostics.

• • Principle of SILAC-Based Co-IP-MS in Protein Interaction Analysis

  Protein-protein interactions are critical components of cellular biological networks, playing essential roles in various biological processes. Investigating these interactions is fundamental for understanding cellular functions and elucidating disease mechanisms. The combination of SILAC (Stable Isotope Labeling by Amino acids in Cell culture) with immunoprecipitation (Co-IP) and mass spectrometry (MS) offers a precise and reliable approach for studying protein-protein interactions.

• • Mechanism of De Novo Antibody Sequencing

  Antibody de novo sequencing (ADNS) is a method used to determine the amino acid sequence of antibodies, which plays a critical role in biomedical research and therapeutic antibody development. Given the widespread application of monoclonal antibodies (mAbs) in treating cancer, infectious diseases, and autoimmune disorders, accurately resolving antibody sequences is crucial for ensuring their efficacy and safety.

• • Workflow of De Novo Antibody Sequencing

  Antibody de novo sequencing is an innovative technology that enables scientists to identify and characterize the amino acid sequences of unknown antibodies. This technique is of great significance in biomedical research and drug development.

• • Advantages and Disadvantages of De Novo Antibody Sequencing

  De novo antibody sequencing has emerged as a pivotal technique in the field of biological research. This technology directly analyzes the amino acid sequences of antibodies, bypassing traditional genomic sequencing methods, and thus provides more direct and accurate data for antibody research and development.

• • Principle of De Novo Antibody Sequencing

  Antibodies are essential molecules in the immune system that specifically bind to antigens, marking foreign substances for clearance by the immune system. With the extensive application of antibodies in both basic research and clinical settings, understanding their sequences and characteristics has become crucial. Antibody de novo sequencing is a technique used to determine antibody sequences without a reference sequence, directly obtaining the amino acid sequence of the antibody through mass spectrometry.

• • Mechanism of Protein Mutation Analysis

  Proteins are essential molecules in biological systems, participating in nearly all cellular processes. Mutations, which are changes in DNA sequences, can alter the amino acid sequences of proteins, thus impacting their structure and function. Analyzing protein mutations is a fundamental aspect of biological research, involving understanding their generation, effects, and detection methods.