Resources
Proteomics Databases
Metabolomics Databases
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• Transcriptome and Proteome Analysis
Transcriptome and proteome analysis are pivotal techniques in modern biological research, playing essential roles in elucidating gene expression and protein functionality within organisms. Transcriptome analysis is focused on examining the expression profiles of all RNA molecules, particularly messenger RNA (mRNA), in cells or tissues. This approach aims to uncover the dynamic changes and regulatory mechanisms underpinning gene expression. Through transcriptome analysis, researchers can identify diffe......
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Whole cell proteomics is a comprehensive approach aimed at studying and analyzing all proteins within a cell. As the fundamental units of life, cells rely on proteins to perform a variety of biological functions. Thus, examining the composition, structure, function, and interactions of cellular proteins enhances our understanding of cellular physiology and pathology. Beyond foundational research, whole cell proteomics holds substantial promise in clinical diagnostics, drug discovery, and precision med......
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SWATH MS analysis is an advanced quantitative proteomics technique designed to achieve high-throughput analysis of a vast array of proteins in complex biological samples through comprehensive spectral acquisition. The primary advantage of SWATH lies in its capacity to analyze nearly all proteins within a sample simultaneously, overcoming the limitations of traditional mass spectrometry that target specific proteins. Unlike conventional tandem mass spectrometry (MS/MS), which conducts secondary analysi......
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SWATH proteomics is an innovative mass spectrometry-based technique designed for comprehensive and precise quantitative analysis of proteins in complex biological samples. While rooted in the principles of tandem mass spectrometry (MS/MS), SWATH proteomics employs a distinct workflow. Traditional mass spectrometry typically targets selected ions, risking the omission of low-abundance proteins. In contrast, SWATH divides the analysis into multiple "windows," simultaneously collecting fragment informati......
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• Single-Cell Functional Proteomics
Single-cell functional proteomics is an advanced technique aimed at studying the protein composition, functional states, and dynamic changes within individual cells. Traditional proteomics often involves analyzing bulk cell populations, which, while informative, can obscure cell-to-cell variability. In contrast, single-cell functional proteomics allows for precise measurement of protein expression, post-translational modifications, and functional properties at the single-cell level, providing a powerf......
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• Single-Cell Proteomic Analysis
Single-cell proteomic analysis aims to investigate the protein composition and dynamics within individual cells, offering detailed biological insights into cellular heterogeneity. Cells are the fundamental units of life, and proteins, as the functional molecules responsible for biological activities, directly influence cellular states and behaviors. In multicellular organisms, even cells of the same type can exhibit significant functional differences, which are often obscured by traditional bulk analy......
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SILAC analysis (Stable Isotope Labeling by Amino Acids in Cell Culture) is a powerful technique for quantitative proteomics. By incorporating stable isotope-labeled amino acids into cellular proteins during growth, SILAC analysis enables accurate in vivo protein labeling, facilitating comparative proteomic studies. This method has broad applications in both fundamental and applied research. In fundamental research, SILAC analysis is extensively used to investigate core biological processes such as the......
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• SILAC Quantitative Proteomics
SILAC quantitative proteomics represents an advanced analytical technique designed to study the dynamic changes of proteins across various biological conditions. This method incorporates stable isotope-labeled amino acids during cell culture, facilitating the precise quantification of target proteins. Specifically, SILAC employs amino acids labeled with stable isotopes such as ^13C and ^15N instead of standard amino acids. Following cell culture, the proteins synthesized are inherently labeled with th......
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SILAC phosphoproteomics represents an advanced methodology that integrates stable isotope labeling with phosphoprotein analysis to investigate the dynamic alterations of phosphorylated proteins within cells. Phosphorylation is a prevalent post-translational modification, playing critical roles in cellular signaling, metabolic regulation, and cell cycle control. Investigating changes in phosphorylated proteins is crucial for elucidating cellular signaling pathways, disease mechanisms, and drug effects.......
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• Proteomics Differential Expression Analysis
Proteomics differential expression analysis is a widely used approach for investigating variations in protein expression levels across different biological conditions. The primary goal of this method is to identify differentially expressed proteins in biological samples that arise due to external stimuli, pathological states, or genetic variations. This analytical strategy has broad applications in life sciences, particularly in disease research, drug development, and biomarker discovery. In disease......
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