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    Resources

      Proteomics Databases

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      Metabolomics Databases

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    • • Glycan Mass Spectrometry Analysis

      In biological sciences, mass spectrometry is widely employed for the qualitative and quantitative analysis of complex samples, such as proteins and nucleic acids. Glycan mass spectrometry refers to the application of mass spectrometry for the characterization of glycans. Glycans are biological macromolecules that are abundantly present in living organisms and play crucial roles in various biological processes, including cell recognition, signal transduction, and immune response.

    • • Number of Disulfide Bonds in a Protein

      Disulfide bonds play a crucial role in protein structure by stabilizing the three-dimensional conformation, which is essential for proper protein function. These bonds are covalent linkages formed between the sulfur atoms of two cysteine residues. Therefore, to estimate the number of disulfide bonds in a protein, it is necessary to identify all cysteine residues and assess their potential pairings.

    • • Overview of Core Technologies for Protein Identification

      Advancements in technology have transformed protein identification from basic qualitative analysis into a comprehensive system integrating multidisciplinary approaches. The primary objective is to characterize protein species, structures, and post-translational modifications (PTMs), facilitating research in life sciences, disease diagnostics, and drug development. The current technological framework for protein identification encompasses mass spectrometry, immunodetection, structural analysis techniqu......

    • • ICAT Mass Spectrometry

      ICAT mass spectrometry is a powerful proteomics technique for quantifying relative protein abundance. In biomedical research, ICAT enables precise protein quantification, offering valuable data for early disease diagnosis and treatment. In drug development, this method facilitates the rapid screening and identification of drug targets, enhancing the efficiency of novel therapeutics. Additionally, in environmental monitoring, ICAT mass spectrometry allows for the precise detection of trace compounds in......

    • • Amino Acid Quantitation

      Amino acid quantitation is a widely employed technique for analyzing and measuring amino acid content in various samples. As the fundamental building blocks of proteins, amino acids play essential roles in numerous metabolic pathways. Beyond their function as protein constituents, amino acids contribute to biomolecule synthesis, regulate biochemical reactions, and maintain osmotic balance in biological systems. Thus, precise quantitation of amino acid composition is critical for understanding physiolo......

    • • ESI-MS Protein Analysis in Proteomics and Biomedical Research

      ESI-MS protein analysis employs electrospray ionization mass spectrometry (ESI-MS) to investigate protein composition, structure, and interactions. This technique plays a pivotal role in proteomics, providing precise molecular weight data and valuable insights into protein function. The core of ESI-MS protein analysis lies in the electrospray ionization process, which transforms protein molecules in liquid samples into gas-phase ions, enabling their detection by mass spectrometry. This approach allows......

    • • Amino Acid Analysis Using HPLC

      Amino acid analysis using HPLC is widely employed for the qualitative and quantitative assessment of amino acids in protein hydrolysates and raw materials. High-Performance Liquid Chromatography (HPLC) is a well-established separation technique in biochemistry and analytical chemistry, enabling the resolution of complex mixtures by exploiting the interactions between the stationary and mobile phases within a chromatographic column.   Amino acids, as fundamental building blocks of proteins, play crucia......

    • • Proteomics Biomarker Discovery

      Proteomics biomarker discovery entails identifying proteins associated with specific diseases or biological states through the analysis of proteomes in cells, tissues, or body fluids. Beyond merely identifying proteins, this process aims to elucidate their functional roles and interactions in disease progression. The significance of proteomics biomarker discovery lies in its potential to enable early disease diagnosis and prognosis assessment. Given that many diseases-particularly cancer, cardiovascul......

    • • Metabolomics Mass Spectrometry

      Metabolomics mass spectrometry is a technique used to study changes in metabolites within organisms. As an important branch of systems biology, metabolomics seeks to reveal metabolic pathways and physiological states by analyzing metabolites in cells, tissues, or whole organisms. These metabolites include amino acids, fatty acids, carbohydrates, nucleotides, and others, which directly participate in and regulate the biological processes of living organisms. Mass spectrometry, a highly sensitive analyt......

    • • BioID&TurboID

      BioID&TurboID are biochemical techniques widely used to investigate intracellular protein interaction networks. These methods enable researchers to map complex protein interaction networks by labeling proteins that are in close proximity to or interact with specific target proteins. BioID, short for Biotin Identification, utilizes a mutant form of biotin ligase (BirA*) to attach biotin tags to nearby proteins. These tagged proteins can then be isolated and identified through affinity capture technique......

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