Resources
Proteomics Databases
Metabolomics Databases
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• N-Terminal Protein Sequencing
N-terminal protein sequencing is a widely used method for determining the amino acid sequence at the N-terminus of a protein. By analyzing the initial amino acid residues of a protein chain, this technique reveals crucial information about protein structure and function, providing insights into biological roles, post-translational modifications, processing mechanisms, and domain architecture. It plays an essential role in proteomics, drug discovery, and biomedical research, particularly in protein str......
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• Co-Immunoprecipitation (Co-IP)
Co-immunoprecipitation (Co-IP) is a well-established technique extensively used in biochemical and molecular biology research to isolate and study target proteins and their interaction partners from complex biological mixtures. The method is based on the specific antigen-antibody interaction, enabling the selective enrichment and purification of target proteins. By leveraging the high specificity and affinity of antibodies, Co-IP effectively isolates target antigens from cell lysates, tissue homogenat......
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De novo antibody sequencing is an advanced analytical technique that leverages high-resolution mass spectrometry (HRMS) and bioinformatics tools to accurately determine the complete amino acid sequence of antibodies. Unlike conventional genomic sequencing or antibody gene library methods, this approach directly analyzes the protein sequence, enabling precise sequence determination without relying on genetic templates. This capability addresses the challenges of recognizing antibody variable regions (C......
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• Protein Analysis Mass Spectrometry
Protein analysis mass spectrometry is a highly precise analytical technique used for the comprehensive study and identification of proteins. Proteins are essential biomolecules that carry out diverse functions in living organisms, including catalyzing biochemical reactions, providing structural support, transmitting cellular signals, and regulating gene expression. Protein analysis mass spectrometry enables scientists to investigate protein structure, function, and interactions with exceptional accura......
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SEC protein purification, short for size-exclusion chromatography, is a widely used technique for separating and purifying proteins, nucleic acids, and other biological macromolecules based on their molecular size differences. At its core, SEC protein purification relies on a column packed with a porous stationary phase. Molecules traverse the column at rates determined by their ability to diffuse into the pores of the matrix. Larger molecules, which cannot penetrate the pores, elute more rapidly, whi......
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Post-translational modification proteomics (PTMs proteomics) is a specialized research field dedicated to investigating chemical modifications occurring after protein synthesis and their functional roles within cellular systems. Protein functionality is determined not only by their amino acid sequences but also by the type, location, and dynamic regulation of post-translational modifications (PTMs). These chemical modifications, including phosphorylation, acetylation, methylation, ubiquitination, and ......
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Kinase profiling is a widely used methodology for investigating the activity of protein kinases and their regulatory roles in cellular signal transduction. Protein kinases are enzymes that catalyze the transfer of phosphate groups from ATP to specific substrate molecules, a process known as phosphorylation. This post-translational modification serves as a fundamental regulatory mechanism in various biological processes, including signal transduction, metabolic regulation, and cell cycle progression. C......
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Proximity proteomics is an advanced research strategy leveraging high-resolution mass spectrometry to investigate the spatial organization and functional dynamics of proteins within cellular environments. Unlike conventional proteomics, which primarily examines global protein expression and modifications, proximity proteomics focuses on the immediate molecular neighborhood and interaction networks of specific proteins with high spatial precision. By precisely labeling target proteins and their proxima......
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LC-MS protein analysis is a powerful bioanalytical technique widely used to characterize complex protein mixtures. By integrating liquid chromatography (LC) and mass spectrometry (MS), LC-MS combines protein separation with molecular weight and structural characterization. In LC, proteins are fractionated into distinct components based on their physical and chemical properties, while MS identifies and analyzes these components with high precision. This technique has become indispensable in biomedical ......
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• Target Validation in Drug Discovery
Target validation in drug discovery is a fundamental step in the drug development process. Its primary objective is to confirm whether specific molecules or proteins are involved in the onset and progression of diseases, and whether they can be targeted for effective drug intervention. During the early stages of drug discovery, target validation in drug discovery lays the foundation for the entire drug development pipeline, ensuring that the research direction is correct and has the potential for clin......
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