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    Resources

      Proteomics Databases

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      Metabolomics Databases

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    • • Secretome Analysis Mass Spectrometry

      Secretome analysis mass spectrometry is a mass spectrometry-based proteomics approach designed to detect and characterize protein components that are actively secreted or passively released by cells, tissues, or body fluids. Secreted proteins play essential roles in intercellular communication, signal transduction, immune regulation, and disease pathogenesis. Secretome analysis mass spectrometry facilitates the elucidation of functional protein networks in biological systems and contributes to researc......

    • • Proteomics Biomarker Analysis

      Proteomics biomarker analysis represents a pivotal methodology in modern life sciences. Biomarkers are quantifiable indicators used to objectively assess biological processes, pathological conditions, or pharmacological responses. In proteomics research, comprehensive protein profiling of biological samples enables the identification of protein biomarkers associated with specific diseases or physiological states. These biomarkers serve as critical tools for early disease diagnosis, prognosis evaluatio......

    • • Chemical Crosslinking Mass Spectrometry

      Chemical crosslinking mass spectrometry, an advancing technique within structural biology research, is extensively employed to elucidate protein-protein interactions (PPI) and protein architectures. The fundamental principle involves utilizing chemical crosslinkers to form covalent bonds between specific amino acid residues within interacting proteins or protein complexes, followed by the identification of these linked sites through mass spectrometric analysis to infer the spatial conformation and int......

    • • De Novo Peptide Sequencing in Research and Industry

      De novo peptide sequencing allows for the determination of amino acid sequences of unknown peptides without prior sequence knowledge. This method is crucial for analyzing complex protein mixtures, thus aiding scientists in understanding biological structures and functions. In the realm of medical research, it plays a pivotal role in identifying disease-related biomarkers, which are essential for the early diagnosis and treatment of conditions such as cancer, cardiovascular diseases, and neurological d......

    • • Chemical Cross-Linking Mass Spectrometry

      Chemical Cross-Linking Mass Spectrometry (CLMS) is an advanced technique in biochemistry and structural biology, combining chemical cross-linking with mass spectrometry to investigate protein-protein interactions and the three-dimensional architecture of protein complexes. The fundamental principle involves using chemical cross-linkers to form covalent bonds between adjacent amino acid residues, thereby preserving spatial information between or within protein molecules. Mass spectrometry then detects ......

    • • Proteomics Mass Spectrometry

      Proteomics mass spectrometry is a fundamental technique in proteomics research, instrumental in identifying and quantifying proteins within biological samples. This approach provides insights into the intricate protein networks within organisms. With the advancement of genomics, genetic sequence data alone are insufficient to capture the organism's complexity and dynamic characteristics. Mass spectrometry in proteomics allows for comprehensive analysis of proteins, facilitating the identification, qua......

    • • Quantifying Western Blots

      Quantifying western blots aims to reveal biological processes by quantitatively analyzing protein expression levels. This technique builds on traditional western blotting but incorporates quantitative analysis, allowing researchers to measure target protein expression with greater accuracy. Quantifying western blots is essentially a quantitative analysis method within western blotting. Similar to Southern blot and Northern blot hybridization techniques, western blotting uses polyacrylamide gel electro......

    • • Protein Profile

      Protein profile refers to the analysis and measurement of the types, quantities, and variations of proteins within a biological system to comprehensively understand their functions and dynamic changes in different biological processes. The application of protein profiling is extensive. During disease onset and progression, the protein profile of biological samples undergoes characteristic changes. For example, in certain cancer patients, the expression levels of specific proteins in serum may increase......

    • • Can LC-MS Detect Enzymatic Glycosylation Modifications

      Liquid chromatography-mass spectrometry (LC-MS) is a powerful tool for detecting enzymatic glycosylation modifications. Glycosylation, a prevalent post-translational modification (PTM) of proteins, is crucial in various biological processes such as cellular signaling, protein folding, and immune recognition. LC-MS is especially advantageous in glycosylation analysis because it provides comprehensive data on glycosylation sites, including the glycosylation type and glycan chain structure.

    • • Peptide Sequencing for Protein Analysis

      Peptide sequencing is a biotechnological method used to elucidate the structural and functional relationships of proteins through analyzing the amino acid sequences in peptide chains. As peptides are fundamental constituents of proteins, understanding their sequences allows researchers to investigate their roles in biological functions more thoroughly. The peptide sequencing process typically involves several critical steps: sample preparation, enzymatic digestion, separation and detection, and data a......

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