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• • Steps and Common Pitfalls in De Novo Protein Sequencing

  De novo protein sequencing is a mass spectrometry-based approach for determining protein amino acid sequences without relying on database references. This technique is widely employed in novel protein identification, antibody sequencing, and post-translational modification (PTM) analysis. While it has significant potential in studies of non-model organisms and protein engineering, challenges persist regarding data quality, algorithmic interpretation, and experimental execution. This review provides a ......

• • Overview of Protein De Novo Sequencing: Break Through Traditional Analysis Limitations

  In proteomics and biopharmaceutical research, determining the amino acid sequence (primary structure) of proteins is essential for elucidating their functions and biological significance. However, conventional protein sequencing methods primarily rely on database searches, making them inadequate for characterizing unknown proteins, novel variants, or unannotated species. As research advances, addressing this limitation has become increasingly critical. Protein de novo sequencing, a technique that dedu......

• • N-Terminal Sequencing: Unveiling the Basics of Edman Degradation and Its Key Applications

  N-terminal sequencing is a technique used to determine the amino acid sequence at the N-terminus of proteins, with extensive applications in proteomics, structural biology, and biopharmaceutical research. Among the available sequencing approaches, Edman degradation remains a classical and widely used method. This technique employs stepwise chemical degradation to sequentially cleave and identify N-terminal residues, enabling direct sequence determination. As a result, it provides valuable insights into.....

• • What Makes De Novo Protein Sequencing So Powerful? A Detailed Exploration

  De novo protein sequencing is a powerful technique for directly determining the amino acid sequences of proteins. It has become an indispensable tool in various fields, including the study of non-model organisms, antibody drug development, and the identification of protein mutations and post-translational modifications. The success of this approach depends on the seamless integration of multiple steps, including sample preparation, mass spectrometry analysis, data interpretation, and algorithm optimization.

• • MS-Based N-Terminal Sequencing: Sample Preparation and Data Analysis

  N-terminal sequencing is a critical approach in proteomics for determining the N-terminal amino acid sequence of proteins and peptides. It plays an essential role in investigating post-translational modifications, protein maturation, degradation pathways, and functional mechanisms. Compared to the traditional Edman degradation method, MS-based N-terminal sequencing has emerged as a powerful analytical tool due to its high sensitivity, high throughput, and compatibility with complex biological samples.......

• • Protein De Novo Sequencing: 10 Key Challenges and How to Overcome Them

  Protein de novo sequencing has shown distinct value in determining unknown amino acid sequences. However, the process still faces several significant challenges, spanning experimental design, data analysis, and biological complexity. These challenges require technological innovations and interdisciplinary approaches to overcome. This paper explores solutions for 5 key issues, integrating the latest advancements in technology.

• • Core Technologies and Latest Development Trends in De Novo Protein Sequencing

  De novo protein sequencing is a mass spectrometry (MS)-based analytical technique that enables the direct determination of a protein’s amino acid sequence without relying on a reference database. Compared to conventional database search methods, this approach facilitates the identification of novel proteins, the characterization of antibody sequences, investigations into post-translational modifications (PTMs), and proteomic studies of non-model organisms. With continuous advancements in mass spectrometry..

• • 7 Powerful De Novo Sequencing Techniques to Unlock Unknown Proteins

  De novo protein sequencing is an innovative technique that allows direct determination of the amino acid sequence of proteins without requiring prior genomic or database references. In contrast to conventional database search approaches, de novo sequencing offers a robust method for investigating unknown proteins, particularly in newly identified biological species, post-translational modification (PTM) analysis, antibody engineering, and biomarker discovery. Recent advancements in mass spectrometry i......

• • Reveal The Core Secret to Mastering N-Terminal Sequencing

  N-terminal sequencing is a fundamental technique in protein research for determining the initial amino acid sequence of a protein. It plays a critical role in protein identification, quality control, and structural characterization. However, achieving precise N-terminal sequencing results requires careful methodological optimization. What are the key factors influencing sequencing accuracy and success rates? In this article, we unveil the core strategies for mastering N-terminal sequencing, equipping ......

• • Optimization of De Novo Protein Sequencing with Mass Spectrometry

  De novo protein sequencing is the process of determining the amino acid sequence of a protein without relying on genomic or protein databases, using high-resolution mass spectrometry. Unlike homology-based sequence analysis, which depends on database comparisons, de novo sequencing is particularly valuable for studying proteins from unknown species, characterizing epigenetically modified proteins, and developing antibody-based therapeutics. By analyzing fragment ion spectra of enzymatically digested p......