Resources

Proteomics Databases



Metabolomics Databases

• • Phosphoprotein Enrichment Methods in Quantitative Phosphoproteomics

  With the rapid development of proteomics, phosphorylation modification, as an essential mechanism regulating cellular physiological functions, has gradually become a research hotspot. Quantitative phosphoproteomics primarily analyzes the phosphorylation status of proteins and their dynamic changes, aiding in the understanding of key biological processes such as signal transduction and cell cycle regulation.

• • Phospho-Site Mapping Based on Mass Spectrometry

  In modern proteomics research, phosphorylation is a key post-translational modification (PTM) that regulates protein activity, interactions, and functions. It plays a significant role in various biological processes, such as cell signaling, metabolism, and cell cycle control. Due to its biological importance, accurate identification and localization of phosphorylation sites are crucial for understanding protein function regulation.

• • Mechanism of Protein N-Glycosylation and its Detection

  N-glycosylation is a crucial form of post-translational modification of proteins, significantly impacting their structure, function, and stability. This process primarily occurs on asparagine (Asn) residues, influencing biological activities, cellular signaling, and immune responses.

• • Application of N-Glycosylation Site Analysis in Biopharmaceutical Development

  N-glycosylation refers to the process of adding sugar chains to the nitrogen atom of proteins, which significantly impacts their structure and function. In biopharmaceutical development, the analysis of N-glycosylation sites provides crucial insights into understanding drug targets' functionality, enhancing drug stability and biocompatibility, and optimizing production processes.

• • Workflow of N-Glycosylation Profiling in Biopharmaceuticals

  N-glycosylation is an essential form of post-translational modification of proteins that significantly impacts the quality and bioactivity of biopharmaceuticals. Understanding the workflow of N-glycosylation is crucial for ensuring the safety and efficacy of biologics.

• • Advantages and Disadvantages of Mass Spectrometry-Based N-Glycosylation Analysis

  N-glycosylation is a widely occurring post-translational modification in living organisms, significantly impacting protein function, stability, and various biological processes such as cell signaling. Mass spectrometry (MS)-based N-glycosylation analysis has emerged as a crucial research tool in this field due to its high sensitivity and throughput.

• • Principle of N-Glycosylation Site Identification

  N-glycosylation refers to the process of attaching glycan chains to the nitrogen atom of asparagine residues in proteins. This modification plays a crucial role in cellular functions and signaling pathways. Understanding the recognition of N-glycosylation sites is essential for elucidating protein functions, interactions, and their roles within biological systems.

• • Mechanism of HILIC-UHPLC in N-Glycosidic Bond Detection

  In biological molecules, carbohydrates play a crucial role as important modifying groups in the functions and structures of proteins. N-glycosidic bonds refer to the connection of carbohydrates to the nitrogen atom of proteins or other biomolecules through glycosidic bonds. Accurate detection and analysis of N-glycosidic bonds is essential for studying glycobiology, glycomics, and related diseases such as diabetes and cancer.

• • Workflow of HILIC-UHPLC in N-Glycosidic Bonds Analysis

  N-glycosidic bonds play a crucial role in various biological processes and diseases, making their analysis essential for understanding carbohydrate biology. High-performance liquid chromatography (HPLC) combined with hydrophilic interaction chromatography (HILIC) provides an effective method for the separation and analysis of N-glycans.

• • Application of HILIC-UHPLC in N-Glycan Structural Analysis

  N-glycans are essential carbohydrate molecules found in glycoproteins and glycolipids, playing crucial roles in various biological processes such as cell recognition, signal transduction, and immune response. In recent years, the importance of structural analysis of N-glycans has increased with the in-depth study of glycoscience.