Resources
Proteomics Databases
Metabolomics Databases
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• Mass Spectrometry in Protein Sequencing
Mass spectrometry in protein sequencing is a technique used to analyze the amino acid sequence of proteins. This method offers detailed structural information about proteins by measuring the mass-to-charge ratio (m/z) of their fragments. With the advancement of genomics, scientists have realized that relying solely on genomic sequences is insufficient to fully understand the complex functions of organisms. Proteins, being the direct executors of cellular functions, are better understood through mass s......
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• Label-Free Quantification Proteomics
Label-free quantification proteomics is an advanced technique used to study protein expression levels and dynamic changes within biological systems. Unlike traditional labeling methods, this technique does not rely on isotopic or chemical labels, but instead directly measures the relative abundance of proteins in a sample using mass spectrometry. This method offers significant advantages for the relative quantification of proteins in complex biological samples, especially in situations involving limit......
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Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis (MALDI-TOF MS Analysis) is a powerful technique used to analyze macromolecules such as proteins, peptides, and polysaccharides. In proteomics, MALDI-TOF MS Analysis is employed to identify and characterize proteins in complex biological samples. By analyzing peptide mass fingerprints, the sequence and structure of proteins can be precisely determined, providing valuable information for disease research and drug devel......
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• Protein Quality Evaluation Methods
Protein quality evaluation methods are essential tools for assessing the nutritional value, bioavailability, and functional properties of proteins. These methods provide critical information for determining how well proteins meet dietary requirements and their potential for use in various food, pharmaceutical, and clinical applications. Current common protein quality evaluation methods include the Chemical Score, Amino Acid Score (AAS), Protein Digestibility-Corrected Amino Acid Score (PDCAAS), Protei......
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Impurity profiling involves identifying, quantifying, and managing non-target components present in samples across disciplines like chemistry, pharmaceuticals, and biotechnology. These impurities may include by-products, degradation products, residual solvents, or other foreign substances from synthesis processes. The objective of impurity profiling is to ensure the sample's purity and stability, thus enhancing the research outcomes' accuracy and reliability. This technique finds applications from bas......
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• High-Performance Liquid Chromatography-Tandem Mass Spectrometry
High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is a sophisticated analytical technique that integrates high-performance liquid chromatography (HPLC) with mass spectrometry (MS) to separate, identify, and quantify target molecules in complex samples. HPLC is utilized for the efficient separation of diverse components within a sample, while MS provides highly sensitive and selective detection through ionization and analysis of the mass-to-charge ratio (m/z) of molecules. Ta......
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Native mass spectrometry is a critical analytical technique for investigating the complex structures and interactions of proteins. Unlike traditional denaturing mass spectrometry, this approach avoids using harsh conditions, such as strong acids, strong bases, or elevated temperatures, which can disrupt the tertiary structure of proteins. This allows researchers to examine proteins and their complexes in a state close to their natural physiological conditions. Native mass spectrometry is widely applie......
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Computational proteomics is a field that integrates bioinformatics, statistics, and computer science with experimental data to analyze, interpret, and predict proteomic characteristics. As high-throughput technologies evolve, the scale and complexity of proteomic studies have expanded, leading to increasingly large and complex datasets. To efficiently process these data and extract significant insights, computational proteomics employs algorithms and computational models, unveiling the structure, func......
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• Advances in Exosome Proteomics Research
Exosome proteomics has emerged as a rapidly advancing field, focusing on the precise quantification and analysis of proteins within exosomes. These small vesicles, secreted by cells and measuring 30 to 150 nanometers in diameter, carry an array of biomolecules, including proteins, RNA, and lipids, and are prevalent in bodily fluids such as blood, urine, saliva, and milk. Exosomes play crucial roles in intercellular communication and are involved in various physiological processes, including immune res......
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LC-MS/MS analysis protein is a powerful technique for identifying and quantifying proteins in complex biological samples. This method combines liquid chromatography (LC) for separating complex mixtures into individual components with mass spectrometry (MS) to measure the mass-to-charge ratio (m/z) of these components. Through collision-induced dissociation (CID) or other fragmentation techniques, secondary ions are generated, providing detailed peptide sequence information for accurate protein sequenc......
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