Resources
Proteomics Databases
Metabolomics Databases
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• Protein Purification Size Exclusion Chromatography
Protein purification size exclusion chromatography (SEC), also known as gel filtration chromatography, is a technique widely used to separate proteins and other biological macromolecules based on their size and shape. In proteomics, precise separation and purification of target proteins are essential for elucidating their structure and function. SEC relies on a porous medium packed within a chromatography column, allowing molecules to traverse different pathways based on their size. Smaller molecules ......
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• Peptide Content Determination
Peptide content determination refers to the precise measurement of peptide levels in a sample using scientific methods. This process allows researchers to accurately assess the concentration and distribution of peptides in a sample, providing vital data for product quality control, clinical treatment planning, and new drug development. As fragments of proteins, peptides possess unique biological activities and are commonly used in early disease diagnosis, therapeutic drug development, and functional i......
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• Protein Characterization Methods
Protein characterization methods encompass a range of techniques, including mass spectrometry, electrophoresis, X-ray crystallography, and nuclear magnetic resonance (NMR) spectroscopy. These methods are essential for understanding protein structure and function in various biological contexts. Mass spectrometry enables precise determination of protein molecular weight and structural features, and is widely utilized in proteomics research. Electrophoresis, on the other hand, is a common method for prot......
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Protein purity analysis refers to the process of determining and evaluating the purity of protein samples using various techniques. In the fields of biochemistry and molecular biology, the purity of proteins directly impacts the accuracy and reproducibility of experimental results. Thus, protein purity analysis is vital for any research or application involving proteins. The main purpose of protein purity analysis is to ensure the effectiveness of downstream experiments and to guarantee the safety and......
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Peptide Content Analysis refers to the measurement of peptide compounds in samples, used for quantitatively analyzing the concentration and composition of peptides in the sample. Peptides are molecules formed by the linkage of amino acids through peptide bonds and are involved in regulating various physiological processes such as hormone secretion, immune responses, and neurotransmission. The biological activity of peptides and their potential in drug development have made peptide content analysis an ......
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• Protein Content Analysis Method
Protein content analysis methods are crucial for diverse fields, including food science, biotechnology, and pharmacology. These methods, including Kjeldahl nitrogen determination, colorimetric assays, UV absorption, HPLC, and mass spectrometry, each have distinct strengths and applications tailored to specific research needs. For instance, the Kjeldahl method remains a gold standard in food industries for measuring total protein content by analyzing nitrogen levels in complex samples. Meanwhile, col......
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• Protein Content Determination
Protein content determination refers to the precise measurement of protein concentration and content in a sample. Numerous methods are available for protein content determination, each with distinct advantages and limitations. The most commonly used methods include colorimetric assays, fluorescence techniques, mass-based methods (such as mass spectrometry), and immunological assays (e.g., ELISA). Accurate protein content determination plays a crucial role in evaluating the nutritional value of f......
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• LC-MS/MS Liquid Chromatography Mass Spectrometry
LC-MS/MS Liquid Chromatography Mass Spectrometry is a technology that integrates Liquid Chromatography (LC) with Tandem Mass Spectrometry (MS/MS). Liquid Chromatography is responsible for separating the components of a mixture, while Mass Spectrometry detects and identifies the molecular weight and structural information of these components. By combining these two techniques, LC-MS/MS achieves high sensitivity and specificity in complex analyses. This method has found widespread applications in variou......
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MS protein sequencing is a technology used for the identification and quantitative analysis of proteins. It determines the amino acid sequence of proteins by analyzing their mass spectrometry data, which provides insights into their structure and function. In disease research, this technology enables the identification of disease-relevant protein biomarkers and aids in uncovering the molecular mechanisms underlying various diseases. These capabilities hold promise for advancing early diagnosis and per......
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• MALDI-TOF MS Bacterial Identification
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Bacterial Identification (MALDI-TOF MS Bacterial Identification) is an advanced technology for microbial identification, offering rapid and accurate bacterial species identification. MALDI-TOF MS Bacterial Identification efficiently classifies and identifies bacteria by analyzing their protein spectra. Compared to traditional biochemical methods, MALDI-TOF MS significantly reduces time and resource consumption, typically prov......
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