Resources
Proteomics Databases
Metabolomics Databases
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Amino acid profiles refer to the composition and relative concentrations of amino acids in biological samples, such as proteins, cells, tissues, or food. Analyzing these profiles is essential for investigating various biological processes, including organism growth, development, metabolism, and disease progression. This analysis method reveals the composition and functional state of proteins by quantifying the types and concentrations of amino acids. Amino acid profile analysis is not only key to nutr......
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• Activity-Based Protein Profiling Overview
Activity-based protein profiling is a method for investigating protein function, mechanism, and their roles in biological systems by detecting and analyzing protein activity. ABPP exploits the ability of small-molecule probes to bind specifically to the active sites of proteins, thereby labeling and enriching active proteins for subsequent identification and functional studies using various analytical techniques. These small-molecule probes typically consist of three components: a reactive group that ......
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• Affinity Based Protein Profiling
Affinity based protein profiling is an effective and versatile technique that enhances our understanding of the complex proteome by identifying and isolating proteins with specific biological functions or interactions. This approach leverages the natural binding properties between proteins, such as antibody-antigen and receptor-ligand interactions, to selectively enrich target proteins. By integrating liquid chromatography with protein mass spectrometry, this technique enables the identification and q......
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2D Gel Proteomics is used for protein separation and analysis. It combines two distinct electrophoresis methods: initially employing isoelectric focusing (IEF) to separate proteins by their isoelectric points, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to further separate them according to molecular weight. This approach can concurrently separate hundreds to thousands of proteins on a single gel, making it particularly suitable for investigating the proteomes of c......
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• N-Terminal Edman Degradation
N-terminal Edman degradation is a widely adopted protein sequencing technique used for analyzing the amino acid sequences of proteins. Renowned for its precision and reliability, it serves as an indispensable tool in biochemical and molecular biology research. The methodology involves the sequential identification of amino acids from the N-terminus, facilitating the elucidation of intricate protein structures. Introduced by Pehr Edman in 1950, this method enables the identification of amino acids with......
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N-terminal Edman sequencing, a method established by Pehr Edman in 1950, remains a cornerstone in protein chemistry for determining the amino acid sequence at the N-terminus of proteins. This method enables precise identification and analysis of the N-terminal amino acids, essential for understanding protein function and structure. The technique hinges on the Edman degradation reaction, wherein the N-terminal amino acid is selectively removed from a polypeptide chain and converted into a detectable de......
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SEC protein analysis is a widely utilized separation technique in proteomics research. The fundamental principle of SEC involves the separation of molecules based on their size as they pass through a gel filtration medium. SEC is effective in the separation and purification of biomacromolecules, such as proteins and nucleic acids, and it plays a crucial role in proteomics research. The primary applications of SEC protein analysis include assessing protein purity, determining molecular weight, analyzin......
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• Analytical Techniques for Protein Characterization
Analytical techniques for protein characterization involve the comprehensive measurement, description, and interpretation of various protein properties to gain a deep understanding of their structure, function, and related characteristics. This analysis is vital for biochemistry, drug development, and clinical diagnosis. Through protein characterization, researchers can explore the three-dimensional structures of proteins, identify functional domains, and elucidate their mechanisms of action both intr......
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SDS-PAGE purity analysis, known as polyacrylamide gel electrophoresis, is a widely utilized technique for the separation and assessment of protein purity based on molecular weight. This method allows researchers to effectively determine protein sample purity, molecular weight, and molecular weight distribution, thereby facilitating further protein analysis. SDS-PAGE is extensively applied in biopharmaceuticals, molecular biology research, and clinical diagnostics. In biopharmaceuticals, the technique ......
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SEC analysis of proteins is a critical chromatographic technique that separates components based on molecular size differences. This separation is accomplished using a stationary phase column packed with porous beads, where the pore size determines the migration speed of molecules. Large molecules, unable to penetrate the pores, elute quickly due to shorter residence times in the stationary phase, whereas smaller molecules enter the pores, resulting in delayed elution times. SEC analysis is essential ......
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