Resources

Proteomics Databases



Metabolomics Databases

• • Peptide Mass Mapping in Proteomics

  Peptide mass mapping is an analytical technique in proteomics. Its core principle relies on mass spectrometry to measure the mass of peptides, allowing researchers to infer their amino acid sequences and corresponding source proteins. By precisely determining peptide masses and integrating bioinformatics analysis, this approach enables both qualitative and quantitative analysis of complex protein mixtures. This is essential for understanding protein function, structure, and interactions within biologi......

• • Applications and Challenges of Native Mass Spectrometry in Proteomics

  Native mass spectrometry is an innovative approach for protein analysis that has garnered increasing attention in proteomics research. As life sciences research continues to advance, proteomics has become an indispensable tool for deciphering biological processes, disease mechanisms, and molecular functions. It plays a pivotal role in fundamental research, clinical diagnostics, and drug development. However, the inherent complexity and diversity of proteins present significant challenges to comprehens......

• • Key Techniques and Development History of Native Mass Spectrometry

  Native mass spectrometry is an advanced technique that enables the direct determination of protein mass, structure, and post-translational modifications without disrupting their native conformation. Unlike traditional enzymatic digestion-based mass spectrometry, native mass spectrometry preserves the high-order structure and multi-subunit composition of proteins during ionization, allowing researchers to analyze protein complexes, molecular interactions, and regulatory mechanisms of protein function w......

• • Native Mass Spectrometry: Principles, Advantages, and Applications

  Native mass spectrometry is an emerging technique in mass spectrometry that allows for the direct analysis of intact protein molecules, providing insights into their native structures and post-translational modifications. It has become increasingly significant in proteomics research. In the following sections, we discuss its fundamental principles, advantages for proteomics, and various applications.   Principles The core principle of mass spectrometry is to ionize the sample and measure its mass-to-c......

• • 5 Essential Protein Identification Strategies to Improve Efficiency

  Protein identification is a crucial analytical process in proteomics research and has been widely applied in life sciences, medical research, and drug development. By employing efficient and precise identification techniques, researchers can characterize the composition, functions, and dynamic changes of intracellular and extracellular proteins. This, in turn, provides valuable insights into early disease diagnosis, personalized treatment strategies, and novel drug discovery. However, protein .......

• • The Ultimate Guide to Protein Identification: Unlock High-Confidence Results

  Accurate protein identification relies on a suite of highly efficient analytical techniques to ensure both the reliability and biological significance of detection outcomes. With the rapid evolution of proteomics, protein identification methodologies have advanced from classical electrophoresis techniques to modern high-resolution mass spectrometry (MS), enabling high-confidence results. In this article, we explore the core technologies of protein identification and their optimization strategies, prov......

• • Strategies, Challenges, and Technological Breakthroughs in Native Mass Spectrometry

  Native mass spectrometry enables the characterization of native protein conformations, dynamic interactions, and complex assembly mechanisms under near-physiological conditions. This technique has revolutionized structural biology by providing unique insights into disease mechanisms and drug development. However, achieving high-fidelity and high-sensitivity analysis of proteins in their native states within complex biological systems remains a major challenge. This paper systematically reviews the str......

• • The Secret to Precise Molecular Analysis: Master Native MS

  Understanding protein structures and interaction mechanisms requires analytical techniques that preserve biomolecular integrity under near-physiological conditions. Native mass spectrometry (MS) has emerged as a powerful tool for studying proteins and their complexes while maintaining their native conformations. As a key technology in precise molecular analysis, native MS enables the characterization of biomacromolecules without disrupting their structural integrity. This article provides a systematic......

• • Limitations and Future Developments of Native Mass Spectrometry Techniques

  Native mass spectrometry techniques have become indispensable tools in proteomics and structural biology research. Compared to conventional denaturing MS, native MS enables the characterization of proteins in their native conformations and complexes under near-physiological conditions, providing biologically relevant insights. However, limitations in sensitivity, complex analysis, and data reproducibility continue to hinder its wider adoption in large-scale studies and clinical applications. Ongoing r......

• • Native Mass Spectrometry: Core Techniques and Recent Advancements

  Native mass spectrometry is a powerful analytical technique that enables the investigation of proteins and their complexes under near-physiological conditions, supporting advancements in proteomics, structural biology, and biomedical research. By maintaining the native conformations of proteins and protein complexes in both solution and gas phases through mild ionization, native mass spectrometry provides critical insights into their assembly states, molecular interactions, and dynamic behavior. In re......