Resources
Proteomics Databases
Metabolomics Databases
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Protein purity analysis refers to the process of determining and evaluating the purity of protein samples using various techniques. In the fields of biochemistry and molecular biology, the purity of proteins directly impacts the accuracy and reproducibility of experimental results. Thus, protein purity analysis is vital for any research or application involving proteins. The main purpose of protein purity analysis is to ensure the effectiveness of downstream experiments and to guarantee the safety and......
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Peptide Content Analysis refers to the measurement of peptide compounds in samples, used for quantitatively analyzing the concentration and composition of peptides in the sample. Peptides are molecules formed by the linkage of amino acids through peptide bonds and are involved in regulating various physiological processes such as hormone secretion, immune responses, and neurotransmission. The biological activity of peptides and their potential in drug development have made peptide content analysis an ......
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• Protein Content Analysis Method
Protein content analysis methods are crucial for diverse fields, including food science, biotechnology, and pharmacology. These methods, including Kjeldahl nitrogen determination, colorimetric assays, UV absorption, HPLC, and mass spectrometry, each have distinct strengths and applications tailored to specific research needs. For instance, the Kjeldahl method remains a gold standard in food industries for measuring total protein content by analyzing nitrogen levels in complex samples. Meanwhile, col......
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• Protein Content Determination
Protein content determination refers to the precise measurement of protein concentration and content in a sample. Numerous methods are available for protein content determination, each with distinct advantages and limitations. The most commonly used methods include colorimetric assays, fluorescence techniques, mass-based methods (such as mass spectrometry), and immunological assays (e.g., ELISA). Accurate protein content determination plays a crucial role in evaluating the nutritional value of f......
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• LC-MS/MS Liquid Chromatography Mass Spectrometry
LC-MS/MS Liquid Chromatography Mass Spectrometry is a technology that integrates Liquid Chromatography (LC) with Tandem Mass Spectrometry (MS/MS). Liquid Chromatography is responsible for separating the components of a mixture, while Mass Spectrometry detects and identifies the molecular weight and structural information of these components. By combining these two techniques, LC-MS/MS achieves high sensitivity and specificity in complex analyses. This method has found widespread applications in variou......
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MS protein sequencing is a technology used for the identification and quantitative analysis of proteins. It determines the amino acid sequence of proteins by analyzing their mass spectrometry data, which provides insights into their structure and function. In disease research, this technology enables the identification of disease-relevant protein biomarkers and aids in uncovering the molecular mechanisms underlying various diseases. These capabilities hold promise for advancing early diagnosis and per......
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• MALDI-TOF MS Bacterial Identification
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Bacterial Identification (MALDI-TOF MS Bacterial Identification) is an advanced technology for microbial identification, offering rapid and accurate bacterial species identification. MALDI-TOF MS Bacterial Identification efficiently classifies and identifies bacteria by analyzing their protein spectra. Compared to traditional biochemical methods, MALDI-TOF MS significantly reduces time and resource consumption, typically prov......
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• Mass Spectrometry in Protein Sequencing
Mass spectrometry in protein sequencing is a technique used to analyze the amino acid sequence of proteins. This method offers detailed structural information about proteins by measuring the mass-to-charge ratio (m/z) of their fragments. With the advancement of genomics, scientists have realized that relying solely on genomic sequences is insufficient to fully understand the complex functions of organisms. Proteins, being the direct executors of cellular functions, are better understood through mass s......
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• Label-Free Quantification Proteomics
Label-free quantification proteomics is an advanced technique used to study protein expression levels and dynamic changes within biological systems. Unlike traditional labeling methods, this technique does not rely on isotopic or chemical labels, but instead directly measures the relative abundance of proteins in a sample using mass spectrometry. This method offers significant advantages for the relative quantification of proteins in complex biological samples, especially in situations involving limit......
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Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis (MALDI-TOF MS Analysis) is a powerful technique used to analyze macromolecules such as proteins, peptides, and polysaccharides. In proteomics, MALDI-TOF MS Analysis is employed to identify and characterize proteins in complex biological samples. By analyzing peptide mass fingerprints, the sequence and structure of proteins can be precisely determined, providing valuable information for disease research and drug devel......
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