Resources
Proteomics Databases
Metabolomics Databases
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• Protein Co-Immunoprecipitation
Protein co-immunoprecipitation (Co-immunoprecipitation, Co-IP) is a classical biochemical technique used to study protein-protein interactions. This method is based on the principle of antigen-antibody specific binding, using specific antibodies to enrich the target protein while simultaneously capturing its interacting proteins, thereby identifying the composition of protein complexes. Protein co-immunoprecipitation enables the study of direct or indirect interactions between proteins under near-physio....
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SP3 (Single-Pot Solid-Phase Enhanced Sample Preparation) proteomics is an efficient, streamlined, and highly automated protein sample preparation technique that has gained widespread adoption in mass spectrometry-based proteomics. Conventional proteomic workflows typically require multiple complex steps, including protein extraction, denaturation, reduction, alkylation, enzymatic digestion, and peptide purification. These processes are not only labor-intensive and time-consuming but also prone to protein...
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Biomarker proteomics involves the application of proteomic technologies to screen, identify, and validate disease-related biomarkers, facilitating disease diagnosis, prognosis assessment, and therapeutic monitoring. Biomarkers are molecular indicators of specific biological processes under physiological or pathological conditions, widely utilized in oncology, cardiovascular diseases, neurodegenerative disorders, and autoimmune diseases. As primary mediators of cellular functions, proteins exhibit ........
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The affinity pull-down assay is a biochemical technique that relies on specific molecular interactions. This method employs a known ligand (such as an antibody, small molecule, protein, or nucleic acid) immobilized on a solid-phase carrier (e.g., agarose or magnetic beads) to selectively bind the target protein in a sample. Following a series of stringent washing steps to remove non-specifically bound molecules, the target protein is ultimately eluted and enriched. Subsequent analysis using mass .......
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Affinity purification-mass spectrometry (AP-MS) protein interaction analysis is a widely used proteomics technique for investigating intracellular protein interaction networks. This method integrates affinity purification with mass spectrometry analysis to selectively isolate target proteins and their interacting partners using affinity-based capture, followed by identification and quantification through high-resolution mass spectrometry. This approach enables a systematic characterization of protein ......
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• Disease Target Identification
Disease target identification is a critical process in elucidating disease pathogenesis and designing novel therapeutic strategies. Disease targets are commonly defined as biomolecules that play a pivotal role in disease progression, including proteins, RNA, and gene mutations, which can serve as direct points of drug intervention. Systematic screening and validation of disease-associated targets provide a scientific foundation for drug development, enhancing therapeutic accuracy and effectiveness. Beyond..
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The pull-down assay is a biochemical technique used to study protein-protein interactions and is widely utilized in molecular biology, cell biology, and biomedical research. This method relies on the specific binding between an affinity tag on a fusion protein and its corresponding immobilized ligand. To perform the assay, researchers first prepare a bait protein tagged with a specific affinity label and immobilize it on a solid-phase affinity matrix. Common affinity tag systems include GST (Glutathione....
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• Drug Target Identification Analysis
Drug target identification analysis is the process of identifying key molecules that specifically bind to drugs and regulate biological functions using biotechnological and computational approaches. Drug targets are predominantly proteins, including receptors, enzymes, ion channels, and transcription factors, which are involved in disease onset and progression. By identifying drug targets, researchers can pinpoint disease-associated proteins and assess their potential as therapeutic targets, thereby .......
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• Phylogenetic Analysis of Protein Sequences
Phylogenetic analysis of protein sequences involves comparing protein sequences from different species or gene families to infer evolutionary relationships. This approach helps elucidate functional changes in proteins and the mechanisms underlying their adaptation during evolution. Initially, phylogenetic analysis was primarily used in taxonomy, where evolutionary trees were constructed based on morphological characteristics. However, with advancements in molecular biology, genetic and protein sequence.....
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• Protein Identification by Peptide Mass Fingerprinting
Protein identification by peptide mass fingerprinting is a mass spectrometry-based approach for efficient protein identification. This method involves enzymatic cleavage of proteins into specific peptides, followed by mass spectrometric analysis to generate a unique peptide mass fingerprint. The resulting fingerprint is then compared with theoretical peptide mass data from protein databases to determine protein identity. Due to its high throughput, rapid analysis, and relatively simple experimental ........
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