Resources

Proteomics Databases



Metabolomics Databases

• • Comparative Analysis of Protein Abundance Using iTRAQ, TMT, and SILAC

  Protein abundance comparative analysis is a critical technique in proteomics research, enabling scientists to understand protein expression levels and their dynamic changes across different conditions. Among the commonly used methods, iTRAQ (Isobaric Tags for Relative and Absolute Quantification), TMT (Tandem Mass Tags), and SILAC (Stable Isotope Labeling by Amino acids in Cell culture) are prominent for their respective advantages and specific applications.

• • New Technologies Drive Expansion in Protein Secondary Structure Analysis

  Protein is an essential molecule in living organisms, and its function is closely related to its structure. The structure of proteins can be divided into four levels, with secondary structure referring to the local spatial arrangement of amino acid residues in proteins. Accurate determination of protein secondary structure is of great significance for understanding protein function and properties.

• • Bioinformatics Analysis of DIA-PRM Proteomics Data

  The rapid advancement of proteomics technology has significantly propelled life sciences research. Proteomics, by analyzing the composition and function of proteins in biological samples, provides essential tools for understanding the dynamics of biological systems. Among these technologies, the combination of Data Independent Acquisition (DIA) and Parallel Reaction Monitoring (PRM) has opened new opportunities for large-scale protein quantification analysis.

• • Comprehensive Insights into Protein Identification Techniques and Future

  Protein is one of the most fundamental components in living organisms and plays a crucial role in understanding their functions and disease mechanisms. The development of protein identification techniques has provided powerful tools for in-depth protein research.

• • In-Depth Analysis of Experimental Steps in Protein Characterization

  Protein is one of the most fundamental functional molecules in organisms, and it is of great significance for understanding the structure and function of biological entities. Protein structure identification is one of the key research areas in the field of biopharmaceuticals.

• • The Principles and Processes of Protein De Novo Sequencing Technology

  De Novo Sequencing is a technique used to determine the amino acid sequence of proteins or peptides solely based on experimental data, without relying on any known protein or nucleic acid databases. This technology is particularly important in the identification of new proteins or modified proteins.   De Novo Sequencing usually utilizes Mass Spectrometry (MS). Proteins or peptides are ionized in the mass spectrometer to produce positive ions.

• • Host Cell Protein (HCP) Detection Methods and Their Principles

  HCP (Host Cell Proteins) refer to protein impurities from the production cells in the process of biological product production. For example, when recombinant proteins are produced using mammalian cells (such as CHO cells), in addition to the target protein, there may be some proteins from the CHO cells themselves in the final product. These HCPs may affect the stability, potency, or safety of the product, so they need to be detected and controlled.

• • FT-IR (Fourier Transform Infrared Spectroscopy) Analysis

  FT-IR (Fourier Transform Infrared Spectroscopy) analysis is a technique widely used for the identification of chemical substances and the study of molecular structures. It is based on the specific absorption of infrared radiation by different chemical bonds and functional groups.   When molecules are irradiated with infrared radiation, they absorb radiation of specific wavelengths, leading to vibrations in the chemical bonds within the molecule.

• • Phosphorylation of Proteins

  Protein phosphorylation is one of the most common and important post-translational modifications in cells. Phosphorylation plays a crucial role in regulating protein activity, subcellular localization, interactions, and stability by adding or removing phosphate groups on specific amino acid residues of proteins.   Basic Process of Phosphorylation The phosphorylation process mainly involves two types of enzymes: protein kinases and protein phosphatases.

• • MtoZ Biolabs: Advanced Post-Translational Modification (PTM) Proteomics Services

  At MtoZ Biolabs, we offer comprehensive proteomic solutions designed to thoroughly analyze PTMs. Our state-of-the-art platform employs advanced mass spectrometry technologies coupled with highly specialized enrichment techniques, providing robust, sensitive, and precise PTM analysis. Our services cover the identification, quantification, and characterization of PTMs, offering valuable insights for academic and pharmaceutical research.