Resources
Proteomics Databases
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Metabolomics Databases
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Protein amino acid analysis is a crucial technique for examining the composition and structure of proteins by determining the types and quantities of amino acids they contain. This analysis offers essential data for understanding protein functions, refining production processes, and developing novel bioproducts. Amino acids, as the fundamental building blocks of proteins, have specific types, proportions, and sequences that dictate protein functionality. This analysis is vital across multiple fields. ......
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• Protein Aggregation Analysis
Protein aggregation analysis is a technique used to study the formation of protein aggregates under specific conditions and their impact on biological functions and the stability of systems. Protein aggregation involves the formation of either insoluble or soluble aggregates through intermolecular interactions due to factors like denaturation, misfolding, or environmental stress. This phenomenon can serve as a normal regulatory process in biological systems or contribute to the pathology of various di......
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PMF proteomics is a protein identification method that utilizes mass spectrometry to analyze the molecular weights of peptide fragments generated by enzymatic digestion of proteins. This process results in a unique "fingerprint" pattern that can be compared against a database for protein identification. PMF proteomics finds applications across various fields. In medical research, PMF is extensively used to identify disease-related proteins, thereby uncovering the molecular mechanisms of diseases. For ......
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• Plant-Based Protein Amino Acid Profile
The plant-based protein amino acid profile analysis is a crucial approach for investigating the structural and functional characteristics of plant proteins. By quantifying and comparing the types, concentrations, and proportions of amino acids in proteins, this method provides essential insights into the biological traits of plants, crop quality improvement, and the development of plant protein resources. As a natural amino acid source, plant proteins play a critical role in human nutrition, animal fe......
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Phosphorylation analysis is an experimental technique integral to proteomics and metabolomics research, focusing on identifying phosphorylation sites, monitoring their dynamic changes, and elucidating their role in biological processes. As a key post-translational modification, phosphorylation is regulated by kinases and phosphatases, which reversibly attach phosphate groups to substrates, thereby modulating their function, activity, and stability. This analysis is pivotal in signal transduction, cell......
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Mascot protein analysis is a widely utilized bioinformatics tool in proteomics for interpreting mass spectrometry data and identifying proteins. This tool functions by comparing experimental mass spectrometry spectra with predicted spectra from known protein databases to identify proteins present in samples. Typically, mass spectrometry data are derived from peptide fragments. Through this approach, researchers can dissect the protein composition of complex biological samples. Mascot's primary role is......
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MALDI-TOF proteomics is an advanced analytical technology combining matrix-assisted laser desorption ionization (MALDI) and time-of-flight mass spectrometry (TOF) for protein identification and analysis. This technique employs a laser to ionize proteins in a sample and accelerates these ions through an electric field, allowing the measurement of their flight times. As ions of different masses have varying flight times, this method can determine a protein's mass through a time-of-flight spectrum. Recog......
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MALDI-TOF peptide analysis employs matrix-assisted laser desorption/ionization-time of flight mass spectrometry for the analysis of proteins and peptides. In proteomics research, this technique is extensively applied for protein identification, relative quantification, and studying post-translational modifications. The core concept involves using laser energy to desorb and ionize peptides from a solid matrix, followed by mass analysis via a time-of-flight spectrometer. For protein identification, the ......
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MALDI protein analysis is an advanced method based on mass spectrometry that employs a laser as an energy source to ionize proteins from solid to gaseous states via matrix molecule absorption and transfer. This technique is highly valued in proteomics for its minimal sample requirements, rapid analysis, and high sensitivity, making it indispensable for determining protein molecular weight, analyzing structures, and identifying samples. MALDI protein analysis is instrumental in biomedicine, drug develo......
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Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a technique that utilizes a laser and a matrix to facilitate the desorption and ionization of sample molecules. During MALDI-MS analysis, the sample is mixed with a matrix material, typically a small molecule compound capable of absorbing laser energy to ionize the sample molecules. Upon laser irradiation, the matrix absorbs the energy, initiating the desorption and ionization process, which generates charged ions from the sam......
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