Resources

Proteomics Databases



Metabolomics Databases

• • How to Design an Absolute Protein Quantification Assay

  Learn how to design an absolute protein quantification assay, from reporting units and AQUA standard strategy to calibration, MRM or PRM optimization, and QC reporting.

• • How to Build a Parallel Reaction Monitoring Assay

  Learn how to build a parallel reaction monitoring assay by selecting proteotypic peptides, optimizing isolation windows, choosing fragment ions, and validating targeted quantitation.

• • Budgeting a Targeted Mass Spectrometry Project

  Learn what drives targeted mass spectrometry project budget, including MRM or PRM platform choice, panel size, matrix complexity, cohort size, and reporting before requesting a quote.

• • AQUA Peptides vs External Standards for Quantification

  Compare AQUA peptides and external standards for absolute quantification by matrix complexity, precision needs, cost, and reporting goals in targeted proteomics.

• • AQUA Peptides: Principles for Absolute Quantification

  Learn how AQUA peptides enable absolute quantification through stable isotope labeling, isotope dilution, calibration design, and targeted MRM or PRM acquisition.

• • Scoping an AQUA Peptide Project: Synthesis, Spike Design, and Validation Deliverables

  Plan an AQUA peptide project with clear synthesis specs, spike design, matrix validation, and reporting deliverables. Avoid standard rework and scope mismatches.

• • AQUA Peptide or Other Internal Standards? Comparing Isotope Dilution Routes for Targeted Quantitation

  Compare AQUA peptides with SILAC, unlabeled spikes, and external calibration for targeted quantitation. Match the standard strategy to your reporting goal.

• • AQUA Peptide Not Performing? Troubleshooting Standard Purity, Spike Level, and Matrix Recovery

  Weak heavy-peptide signal or unstable ratios in AQUA peptide assays? Learn how to troubleshoot standard purity, spike level, storage, and matrix recovery.

• • AQUA Peptides for Absolute Quantitation: How Stable Isotope Standards Convert Targeted LC-MS Signal to Known Amounts

  Learn how AQUA peptides use stable isotope labels and isotope dilution in MRM or PRM assays to report protein and peptide amounts in defined units.

• • Absolute vs Relative Protein Quantification

  Compare absolute and relative protein quantification by reporting goal, calibrator needs, study stage, and documentation depth to choose the right MS workflow.