Resources

Proteomics Databases



Metabolomics Databases

• • Charge Variant Analysis of Monoclonal Antibodies

  Charge variant analysis of monoclonal antibodies (mAbs) is a vital technique in proteomics, primarily used to detect and characterize charge variants in monoclonal antibodies. These charge variants arise from chemical modifications or mutations in the amino acid residues of the antibody structure, leading to shifts in positive or negative charges. Such changes may result from minor variations in manufacturing processes, chemical modifications (e.g., glycosylation, deamidation, oxidation), or protein d......

• • Host Cell DNA

  Host cell DNA, the genetic material present within host cells, holds significant importance in the field of biology. It encompasses all the genetic information necessary for guiding the growth, division, and functionality of an organism's cells. Structurally, this DNA is composed of a double helix formed by four nucleotides: adenine, guanine, cytosine, and thymine, connected by phosphodiester bonds into long chains. The coding sequences of genes within the DNA influence protein synthesis in host cells......

• • Polysaccharide Sequencing

  Polysaccharide sequencing is an analytical technique used to determine the monosaccharide composition, linkage patterns, and branching structures of polysaccharide molecules. In the fields of biochemistry and molecular biology, polysaccharides are known for their structural complexity and functional diversity, which makes sequencing essential for understanding their roles in biological systems. Polysaccharides are crucial in cellular architecture, energy storage, signal transduction, and biological pr......

• • N- and C-Terminal Sequencing

  N- and C-terminal sequencing are techniques used to analyze the amino acid sequences at the amino (N-terminal) and carboxyl (C-terminal) ends of protein molecules. Proteins, composed of amino acids linked by peptide bonds, possess distinct biological functions. The sequences at their N- and C-terminals are crucial in defining their structural and functional attributes. The N-terminal sequence begins with a free amino group, whereas the C-terminal sequence concludes with a free carboxyl group. By exami......

• • MS/MS Peptide Sequencing

  MS/MS peptide sequencing, or tandem mass spectrometry peptide sequencing, is a pivotal technique in proteomics research. Given that proteins are fundamental to life functions, any alteration in their structure or function can significantly impact an organism's health and disease states. This technique involves ionizing protein samples with a mass spectrometer and fragmenting them into peptides through sequential mass spectrometry analysis. By examining the mass of these fragments, researchers can reco......

• • PTM Analysis by Mass Spectrometry

  PTM analysis by mass spectrometry is a powerful technique in proteomics that provides detailed insights into the complex regulatory mechanisms of proteins. Post-translational modifications (PTMs) refer to covalent changes that occur after protein synthesis, such as phosphorylation, glycosylation, and acetylation. These modifications can profoundly influence protein function, stability, and cellular localization. Thanks to its high sensitivity, resolution, and throughput, mass spectrometry has become t......

• • Proximate Analysis of Protein

  Proximate analysis of protein refers to the process in proteomics research that employs a range of bioinformatics and experimental techniques to compare and analyze similar protein sequences or structures, with the aim of uncovering their biological functions, evolutionary relationships, and structural characteristics. This approach involves evaluating sequence similarity, structural similarity, and functional similarity. Proximate analysis of protein is commonly used to predict the function of unknow......

• • Protein Target Identification

  Protein target identification is an essential step in proteomics research, aimed at identifying and validating proteins involved in specific biological processes, disease states, or drug interactions. Protein targets are those proteins that perform specific functions within and outside cells and may act as critical regulators in diseases or as direct drug targets. By elucidating the relationships between these proteins and their biological functions, scientists gain a deeper insight into cellular mech......

• • Protein Structure Identification: Methods and Challenges

  Protein structure identification refers to the determination of proteins' three-dimensional structures through experimental techniques to gain insights into their functional properties and biological roles. Proteins are pivotal in biological processes, acting as catalysts in enzymatic reactions or as receptors in signal transduction. Thus, understanding protein structures is vital for comprehending their functions. Structural identification not only elucidates how proteins interact with other molecule......

• • Protein Structure Alignment and Analysis

  Protein structure alignment and analysis are crucial techniques in bioinformatics and structural biology. These techniques aim to elucidate evolutionary, functional, and sequence relationships by comparing the three-dimensional structures of proteins. Proteins, as complex molecules formed by folded amino acid chains, have their biological functions determined directly by their three-dimensional structures. Understanding these structures allows scientists to infer how proteins achieve their specific fu......