Resources

• • Protein-Protein Interaction in Biological Processes

  Protein-protein interaction (PPI) refers to the process by which two or more protein molecules form complexes through non-covalent binding. This interaction is fundamental to nearly all biological processes within living organisms. Proteins are the primary functional molecules in cells, performing critical roles such as enzyme catalysis, signal transduction, structural support, and material transport. Within each cell, a complex network of interactions coordinates diverse biological activities. For in......

• • Amino Acid Test

  Amino acid test aims to accurately measure the content and composition of amino acids in biological samples. As the core constituents of proteins, amino acids play an essential role in biological processes. Consequently, their precise quantification is vital for both life science research and clinical diagnostics. Amino acid testing helps researchers gain a deeper understanding of protein structure, function, and metabolic pathways, and it has broad applications across various fields. Through accurate......

• • SWATH Mass Spec

  SWATH mass spectrometry is an advanced data-independent acquisition technique that has attracted considerable attention in proteomics research in recent years. This technology addresses the challenge of inadequate coverage of low-abundance proteins in traditional data-dependent acquisition approaches by fragmenting all ions within a predefined mass range. The introduction of this technique has significantly enhanced the coverage and reproducibility of quantitative proteomics data, as well as improved ......

• • Metabolomics and Proteomics

  Metabolomics and proteomics provide complementary insights into biological activities at the molecular level, with metabolomics focusing on small molecule metabolites and proteomics on proteins. These two fields are fundamental to modern biological research. Metabolomics studies metabolites, which are intermediate or final products of biochemical reactions. The dynamic fluctuations in metabolite levels reflect cellular metabolic states and responses to environmental changes. By analyzing the types, co......

• • Bottom Up Proteomics

  Bottom up proteomics is a widely used strategy for qualitative and quantitative analysis of proteins in proteomics research. Unlike top-down proteomics, the core principle of bottom up proteomics involves digesting the entire protein sample into smaller peptides prior to analysis. These peptides are then analyzed in detail by mass spectrometry (MS). This process allows for the precise investigation of protein structure, function, and dynamic changes, facilitating the efficient analysis of thousands of......

• • Molecular and Cellular Proteomics

  Molecular and Cellular Proteomics is a scientific discipline that investigates the composition, structure, function, and dynamic changes of proteins within living systems. Its goal is to uncover the mechanisms through which proteins function at the molecular and cellular levels. As a cutting-edge field in life sciences, it integrates mass spectrometry, advanced separation techniques, and bioinformatics, providing powerful tools for probing complex biological systems. By analyzing the expression profil......

• • Decoding Plant Roots with Single-Cell Precision: Single-Cell Proteomics Reveals Arabidopsis Cell Functions

  Single-cell proteomics (SCP) is a cutting-edge analytical technique that deciphers protein composition and function at the resolution of individual cells. By uncovering cellular-level details, single-cell proteomics reveals intercellular heterogeneity and addresses limitations of traditional bulk proteomics, which provide only average or aggregate protein expression data. In plant research, single-cell proteomics circumvents challenges posed by rigid cell walls that hinder traditional methods from acc......

• • Quantitative Mass Spectrometry

  Quantitative mass spectrometry is primarily employed to measure the concentrations of proteins or other molecular components in biological samples, providing insights into their roles in specific biological processes or disease states. This technique utilizes a mass spectrometer to analyze the mass, abundance, and other characteristics of target molecules with high sensitivity. By integrating standardized quantitative methods, it enables precise measurement of molecular information in complex biologic......

• • Circular Dichroism for Proteins

  Circular dichroism for proteins is a spectroscopic technique widely used to analyze the secondary structures of proteins, their conformational changes, and interactions with ligands. This method leverages the optical phenomenon of circular dichroism, which arises from the differential absorption of left- and right-handed circularly polarized light by chiral molecules. By recording CD spectra across various wavelengths, researchers can deduce the secondary structure composition of proteins, including α......

• • CD Spectroscopy of Proteins

  CD spectroscopy of proteins is a versatile and widely used technique for investigating the conformational and dynamic properties of biological macromolecules. By measuring the differential absorption of left- and right-handed circularly polarized light, it provides critical insights into molecular conformation. Since the secondary structure of proteins directly influences circular dichroism spectra, this method finds broad applications in monitoring conformational changes, studying stability, analyzin......