Histone Post-Translational Modification Analysis Service

Histones are highly conserved proteins, and histone subtypes have highly similar amino acid sequence. The N-terminal ends of histones can be modified by several types of PTMs, including acetylation, methylation, phosphorylation and so on, which affect the DNA transcription, replication, and DNA condensation, etc. Therefore, analysis on histone N-terminal modifications can provide valuable information for epigenetic studies. MtoZ Biolabs has optimized our sample preparation protocols to obtain highly purified histones with the least effect to the modification. We also use 2-3 enzymes for protein digestion to ensure full coverage analysis of histone sequence, eliminating any missing information of short peptides rendered by using only one enzyme.

MtoZ Biolabs has developed a specialized platform, equipped with Q Exactive HF (Thermo Fisher), Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers, equipped with Nano-LC for histone modification analysis service. To better solve your research problems, we offer an all-inclusive service, including sample preparation, protein purification, digestion, peptide separation, LC-MS/MS analysis, histone modification analysis and bioinformatics analysis. All you need to do is to tell us your project objective, and send us your cell samples, and we will complete all the following experiments.

 

Analysis Workflow

 


 

Sample Submission Requirements

 


*Note: We will perform testing experiment before we start official experiments. To ensure the most cost-effective and accurate analysis is provided, only qualified samples will proceed to the official analysis.

 

Deliverables

1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. Peptide Identifications and Intensities
5. Protein Identifications and PTMs Mapping
6. Bioinformatics Analysis

 

Related Services

PTM Analysis

Phospho Proteomics
Acetyl Proteomics
Ubiquitin Proteomics
Glyco Proteomics
Disulfide Bond
Histone Modifications



Protein Analysis

Protein Identification
Protein Mass Measurement
PTMs Identification



Protein Sequencing

Protein De Novo Seq
N-Terminal Sequencing
C-Terminal Sequencing
Edman Degradation
Protein Full-Length Sequencing

• • Histone Propionylation Analysis

  Histone propionylation is a significant post-translational modification characterized by the addition of a propionyl group to the lysine residues of histone proteins. This modification is a key regulator of chromatin structure and function, playing a crucial role in vital biological processes such as metabolic regulation and cellular stress responses.

• • Histone Malonylation Analysis

  Histone malonylation represents an emerging and significant post-translational modification (PTM) characterized by the covalent attachment of malonyl groups to lysine residues within histone proteins. This modification is increasingly recognized for its critical role in modulating chromatin architecture and regulating gene expression.

• • Histone Trimethylation Analysis

  Histone trimethylation is a pivotal post-translational modification (PTM) that involves the addition of three methyl groups to specific lysine residues on histone proteins, which are crucial components of chromatin structure. This modification is recognized for its ability to influence gene expression by altering chromatin dynamics and accessibility. Trimethylation can occur on various lysines, including H3K4, H3K9, H3K27, H4K20, and H4K36, each associated with distinct regulatory functions.

• • Histone Phosphorylation Analysis

  Histone phosphorylation is a well-known post-translational modification (PTM) in which specific kinases catalyze the covalent addition of phosphate groups to serine, threonine, or tyrosine residues on histones. This modification is reversible, as phosphatases can remove the phosphate groups. Histone phosphorylation plays a central role in processes such as epigenetic regulation, modulation of gene expression, DNA damage repair, and cell cycle control. Given its reversible and dynamic nature, phosphorylation

• • Histone Succinylation Analysis

  Histones are a highly conserved class of proteins, with only minor differences in amino acid sequences between their various variants. The N-terminal region of histones can undergo several types of modifications, including methylation, acetylation, phosphorylation, ubiquitination, and ADP-ribosylation. These covalent modifications are fundamentally involved in regulating gene transcription, DNA repair, replication, and chromosome condensation. Among these, histone succinylation is a newly identified post-tr

• • Histone Ubiquitination Analysis

  Histones are a highly conserved class of proteins, with only minor differences in amino acid sequences between their various variants. The N-terminal region of histones can undergo several types of modifications, including methylation, acetylation, phosphorylation, ubiquitination, and ADP-ribosylation. These covalent modifications are fundamentally involved in regulating gene transcription, DNA repair, replication, and chromosome condensation. Among these, histone ubiquitination represents a critical post-t

• • Histone Butyrylation Analysis

  Histone butyrylation is a critical post-translational modification (PTM) of histone proteins, characterized by the addition of a butyryl group to lysine residues in the histone tails. This modification is closely related to butyric acid, a short-chain fatty acid produced through fermentation by gut microbiota, and plays a significant role in various metabolic pathways.

• • Histone β-Hydroxybutyrylation Analysis

  Histone β-hydroxybutyrylation is a prevalent post-translational modification found on lysine residues of histones, originating from β-hydroxybutyrate (BHB), one of the key metabolites associated with ketone metabolism. This modification has garnered significant attention due to its critical role in regulating gene expression and its implications in various biological processes.

• • Histone 2-Hydroxyisobutyrylation Analysis

  Histone post-translational modifications (PTMs) play a critical role in the regulation of gene expression and cellular functions. The N-terminal lysine (Lysine, K) residues are primary targets for various modifications, including acetylation, methylation, and ubiquitination. Histone 2-hydroxyisobutyrylation (2-HIB) is an important post-translational modification characterized by the addition of a hydroxyisobutyryl group to the N-terminal lysine residues.