Quantitative Ubiquitinomics Service
- Plant development and stress response
- Studies on infection and immunity
- Research on aging, regeneration, and degenerative diseases
Ubiquitination is an important PTM in cell biology, as the ubiquitinated proteasome complex has mediated degradation of 80-85 percent proteins, and directly affects the expression level and function of proteins. MtoZ Biolabs is glad to offer ubiquitination identification and quantification service through high-resolution mass spectrometry analysis. For some low abundance ubiquitinated proteins, we can provide peptide enrichment service before mass spectrometry analysis. To ensure high specificity and efficacy of enrichment of ubiquitinated peptides, we use CST Ubiquitin antibody for enrichment process, and use 2-3 different enzymes for protein digestion to ensure full scan of ubiquitinated peptides.
MtoZ Biolabs has developed a specialized platform equipped with Q Exactive HF (Thermo Fisher), Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers, equipped with Nano-LC for ubiquitinated proteomics analysis service. To better solve your research problems, we offer an all-inclusive service, including protein purification, digestion, peptide enrichment and separation, LC-MS/MS analysis, MS data analysis and bioinformatics analysis. All you need to do is to tell us your project objective, and send us your cell/protein samples, and we will complete all the following experiments.
Technical Principles
The lysine residues that undergo ubiquitination are covalently attached to two glycine residues (K-ε-GG), resulting in a mass shift of 114.1 Da. This mass shift can be precisely detected by mass spectrometry, allowing for the identification of ubiquitinated peptides and their modification sites.
Analysis Workflow
Service Advantages
1. High-Throughput Analysis, Identify and Quantify up Thousands of Proteins at Once
2. Quantitatively Analyze Low Abundance Ubiquitinated Proteins
3. Use 2-3 Enzymes for Protein Digestion, Ensure Full-Coverage Analysis
4. State-of-Art Nano LC and Orbitrap Fusion Mass Spectrometer
Sample Submission Requirements
*Note: We will perform testing experiments before we start official experiments. To ensure the most cost-effective and accurate analysis is provided, only qualified samples will proceed to the official analysis.
Applications
Deliverables
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. Peptide Identifications and Intensities
5. Protein Identifications and Ubiquitin Mapping
6. Bioinformatics Analysis
Related Services
Phospho Proteomics
Acetyl Proteomics
Ubiquitin Proteomics
Glyco Proteomics
Disulfide Bond
Histone Modifications
Protein Identification
Protein Mass Measurement
PTMs Identification
Protein De Novo Seq
N-Terminal Sequencing
C-Terminal Sequencing
Edman Degradation
Protein Full-Length Sequencing
How to order?
