Post-Translational Modifications Proteomics Service
Post-translational modifications (PTMs) are covalent and usually enzymatic modifications on proteins. Common PTMs include phosphorylation, acetylation, ubiquitination, glycosylation, and so on. These PTMs can cause huge influences on protein structure, distribution, and function, hence, increasing the complexity of proteome to a greater extent. Protein PTMs is generally analyzed by measuring the mass increased on the modified peptides. Since PTMs are generally present in very low abundance, specific enrichment procedures are required before PTMs identification. MtoZ Biolabs has established a powerful and professional Post-Translational Modifications Proteomics Service, which includes Thermo Fisher Q ExactiveHF and Obitrap Fusion Lumos mass analyzer system, coupled with Nano-LC system. Our aim is to provide the most professional support for our clients’ research.
Analysis Workflow
PTMs Analysis Service Categories
MtoZ Biolabs provides protein phosphorylation analysis service with highly efficient phospho-peptides enrichment processing and Nano LC-MS/MS analysis. Coupled with SILAC/iTRAQ labeling, this service can be applied to large-scale phospho-proteomics analysis.
MtoZ Biolabs provides precise acetyl-proteomics analysis service using Nano LC-MS/MS. We also use CST acetylation-specific antibodies for acetyl-peptide enrichment, and 2-3 different enzymes for protein digestion to ensure full scan of acetyl-peptides.
MtoZ Biolabs offers protein ubiquitination identification and quantification service using high-resolution mass spectrometry analysis. We also use highly specific ubiquitin antibody for enrichment of low abundance ubiquitin-peptide.
MtoZ Biolabs utilizes the HCD/ETD mode of Orbitrap Fusion mass spectrometry for glycoprotein analysis. Coupled with Byonic software, we can accurately analyze N- and O-linked glycosylation sites and the corresponding glycogens.
MtoZ Biolabs provides disulfide bond analysis service at both single protein level and proteome level. We have optimized our sample preparation method to reduce the chance of in vitro disulfide bond exchange and maintain the native protein structure to the greatest extent.
MtoZ Biolabs has optimized our sample preparation protocols to obtain highly purified histones with the least effect to the modification, and ensures precise histone modification analysis using Nano LC-MS/MS.
*Note: We also provide custom analytical service to identify many other types of PTMs. For special requirement, please contact us for project discussion.
Sample Submission Requirements
*Customers are welcome to contact us for detailed sample requirements before sending your samples.
Case Study
In this study, the ubiquitination modification sites of a Co-IP protein sample are analyzed. Part of the final analytical results is listed as below, showing the peptide sequences and identified ubiquitination sites respectively.
Deliverables
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. Identification of Peptides with PTMs
Related Services
Protein Analysis
Protein Identification
Protein Mass Measurement
LC-MS Analysis of Pull-Down Proteins
Native MS Analysis
Protein Sequencing
Protein De Novo Seq
N-Terminal Sequencing
C-Terminal Sequencing
Edman Degradation
Protein Full-Length Sequencing
PTM Analysis
Phospho Proteomics
Acetyl Proteomics
Ubiquitin Proteomics
Glyco Proteomics
Disulfide Bond
Histone Modifications
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• Protein Nitrosylation Analysis
Post-translational modifications (PTMs) are chemical changes that occur to proteins during or after translation. These modifications are essential for various cellular processes, including cell differentiation, protein degradation, signal transduction, gene expression regulation, and protein interactions. One significant type of PTM is protein nitrosylation, which involves the addition of nitrosyl groups to specific amino acids, influencing the protein's structure and functionality. This modification is cru
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• Protein N-Glycosylation Analysis
Post-translational modifications (PTMs) of proteins encompass the chemical modifications that take place during or after the translation of proteins. These modifications are crucial in various cellular processes, including cell differentiation, protein degradation, signal transduction, regulatory processes, gene expression modulation, and protein interactions. One significant form of PTM is N-glycosylation, wherein sugar chains are attached to proteins via the nitrogen atom of amino acid residues, predomina
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• Protein Palmitoylation Analysis
Protein post-translational modifications (PTMs) refer to the chemical modification process of proteins during or after translation. Protein post-translational modifications play a key role in many cellular processes, such as cell differentiation, protein degradation, signal transduction and regulatory processes, gene expression regulation, and protein-protein interactions. Protein palmitoylation refers to the modification process of adding one or more palmitic acids (C16 fatty acids) to the amino acid resid
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• Protein Benzoylation Analysis
Leveraging state-of-the-art mass spectrometry and advanced proteomic analysis, MtoZ Biolabs offers highly sensitive Protein Benzoylation Analysis services to help researchers investigate this emerging modification and its diverse biological functions.
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• Protein Carboxyethylation Analysis
MtoZ Biolabs, utilizing advanced mass spectrometry platforms and specialized proteomic techniques, provides highly sensitive and specific protein carboxyethylation detection and quantification services, facilitating the detailed investigation of this modification in both physiological and pathological contexts.
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MtoZ Biolabs offers highly sensitive and specific Protein Nitration Analysis services based on advanced liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology. We provide two main services: Target Protein Nitration Analysis, which uses immune enrichment combined with LC-MS/MS to accurately identify nitration sites on specific proteins, assisting researchers in studying their roles in signal transduction and cellular stress responses.
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• Protein Diglycylation Analysis
MtoZ Biolabs offers highly sensitive and specific Protein Diglycylation Analysis services, leveraging advanced liquid chromatography-mass spectrometry (LC-MS/MS) platforms. Our services allow for precise detection of diglycylation sites, helping researchers gain deeper insights into the functions and regulatory mechanisms of this modification in diverse biological contexts.
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• Protein β-Hydroxybutyrylation Analysis
Post-translational modifications (PTMs) refer to chemical alterations that occur during or after protein synthesis and play critical roles in cellular processes such as differentiation, degradation, signal transduction, gene expression regulation, and protein interactions. β-Hydroxybutyrylation (Kbhb), a novel PTM, occurs under conditions of β-hydroxybutyrate (BHB) accumulation and involves the covalent attachment of a β-hydroxybutyryl group to lysine residues. This modification plays a key role in regulati
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• Protein 2-Hydroxyisobutyrylation Analysis
Post-translational modifications (PTMs) involve chemical changes to proteins during or after their synthesis, playing crucial roles in cellular processes such as differentiation, degradation, signal transduction, gene expression regulation, and protein-protein interactions. 2-Hydroxyisobutyrylation (2HIB), a novel PTM first identified in histones, involves the covalent attachment of a 2-hydroxyisobutyryl group to lysine residues in proteins. This modification significantly impacts gene expression, metabolic
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• Protein Malonylation Analysis
Post-translational modifications (PTMs) are chemical alterations that occur during or after protein synthesis and are pivotal in cellular processes such as differentiation, degradation, signal transduction, gene expression regulation, and protein-protein interactions. Malonylation, a significant PTM, modulates protein function, stability, and interactions, thereby influencing various cellular activities. It alters protein structure, facilitating or preventing binding with specific molecules, which regulates
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