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Proteomics Databases



Metabolomics Databases

• • How to Measure the Extinction Coefficient of a Fluorescent Protein

  The extinction coefficient of fluorescent proteins (FPs) is determined using a spectrophotometer, which measures their concentration by analyzing absorbance at specific wavelengths. The general procedure involves three steps: calculating the theoretical molar extinction coefficient, preparing the sample, and applying the Beer-Lambert law.

• • The Range of Molecular Weight Measured by MALDI-TOF

  Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) is a sophisticated analytical technique employed for determining ion masses. This method is capable of measuring a wide array of molecular masses, ranging from small molecules and oligonucleotides to peptides, proteins, and even large polymers and complexes. Molecular Mass Range: MALDI-TOF MS surpasses other mass spectrometry techniques in its ability to analyze an extensive range of molecular masses. Ideally, it....

• • Serum Type IV Collagen Assay

  Serum type IV collagen is a distinct collagen located primarily within the basement membranes of the human body, serving as a vital component of the extracellular matrix. Its measurement is principally applied in the evaluation of renal diseases such as chronic kidney disease and diabetic nephropathy, as well as other conditions associated with basement membrane impairment, including liver cirrhosis and heart failure.

• • ICP-MS Determination of Mercury

  ICP-MS (Inductively Coupled Plasma Mass Spectrometry) is a highly versatile analytical technique used to quantify elemental concentrations and isotopic ratios with exceptional sensitivity and precision. Its capability to detect a wide range of elements with high accuracy has made ICP-MS an indispensable tool in fields such as environmental science, geology, biology, and materials science. Outlined below are the steps involved in the quantification of mercury using ICP-MS:

• • HCP Coverage Test

  Host cell proteins (HCPs) refer to proteins that are unintentionally expressed by host cells used in the production of target proteins during biopharmaceutical manufacturing. These contaminating proteins may compromise the safety or efficacy of the drug, and therefore, their levels must be monitored and controlled through rigorous testing. Importance of HCP Coverage Testing HCP coverage testing evaluates the ability of antibody-based methods to efficiently detect various HCPs. The results from this test....

• • Bradford Protein Content Determination Experiment Report

  Objective: The aim of this experiment is to quantify protein concentration via the Bradford assay. Principle: The Bradford assay is based on the binding of Coomassie Brilliant Blue G-250 to proteins, forming a stable blue complex. The absorbance of this complex peaks at 595 nm when in an acidic environment, enabling the determination of protein concentration.

• • Type IV Collagen Test

  Significance of Type IV Collagen Detection: Type IV collagen is a crucial component of the basement membrane, serving as a primary structural element in numerous organs and tissues. It plays an essential role in various physiological processes such as cell migration, growth, differentiation, apoptosis, and disease progression. Consequently, Type IV collagen detection is extensively utilized in clinical diagnostics and disease research.

• • Numerical Y-Coordinate of Circular Dichroism Spectrum

  In circular dichroism (CD) spectroscopy, the vertical axis represents either the absorbance or transmittance of light, determined by the ratio of the intensity of light passing through the sample to that passing through a reference material. This ratio is commonly referred to as "transmittance," denoted by "T," or "absorbance," denoted by "A," with absorbance often being the logarithmic form of the transmittance ratio. Circular dichroism is an analytical technique that assesses the chemical composition a...

• • Sample Loading Requirements for Circular Dichroism Spectroscopy Protein

  Circular dichroism (CD) spectroscopy is a technique used to analyze protein structures based on their interaction with circularly polarized light, enabling the study of protein folding and conformational changes. Accurate sample preparation is crucial for obtaining high-quality results in such analyses.

• • Host Residual DNA Detection

  In the fields of biological research and bioproduct manufacturing, particularly during biopharmaceutical production, the detection of host residual DNA represents an indispensable quality control measure. Residual DNA from host organisms may persist through production processes, potentially compromising the quality, safety, and therapeutic efficacy of the final product.