Resources

Proteomics Databases



Metabolomics Databases

• • Targeted vs Untargeted Metabolomics Approaches

  Metabolomics is a core discipline within systems biology, focusing on the comprehensive characterization of small-molecule metabolite dynamics in biological systems. With the rapid advancement of mass spectrometry technologies and computational data analysis tools, targeted metabolomics and untargeted metabolomics have emerged as two principal analytical strategies widely applied in disease mechanism studies, drug development, nutritional research, and environmental toxicology. Targeted vs Untargeted......

• • Advantages and Disadvantages of Untargeted Metabolomics

  In life science research, metabolomics serves as a critical bridge between genotype and phenotype. As the final downstream products of biological processes, metabolites most directly reflect the integrated responses of cells, tissues, or whole organisms to genetic regulation, environmental stimuli, and disease states. Among current analytical strategies, untargeted metabolomics, characterized by broad analytical coverage and a high potential for novel discovery, has been widely applied in disease mech......

• • Techniques of LC-MS Molecular Weight Determination

  In life science research and biopharmaceutical development, accurate molecular weight determination constitutes a fundamental basis for structural identification, quality control, and drug development. Whether assessing the completeness of protein expression or verifying the purity of synthesized small molecules, molecular weight information represents an essential analytical parameter. Among the available analytical approaches, liquid chromatography-mass spectrometry (LC-MS) has been extensively appl......

• • What Organelle Proteomics Techniques Are Used in Modern Cell Biology?

  In modern cell biology research, organelle proteomics has emerged as a powerful approach for investigating subcellular functional organization, protein localization, and intracellular signaling pathways. By leveraging high-throughput mass spectrometry technologies, researchers can systematically identify and quantify organelle-specific proteomes, thereby uncovering their dynamic regulation and functional specialization. This article provides a comprehensive overview of the major organelle proteomics t......

• • Advantages of ESI-MS in Peptide Drug Molecular Weight Analysis

  With the continuous advancement of biopharmaceutical technologies, peptide drugs, characterized by high specificity, favorable biocompatibility, and relatively low immunogenicity, have emerged as an important therapeutic strategy for cancer, autoimmune disorders, and metabolic diseases. In the development of peptide-based therapeutics, molecular weight analysis represents a fundamental and indispensable step. Owing to their structural complexity, peptide molecules are susceptible to interference from ......

• • What Is LC-MS Molecular Weight Analysis? Applicable Targets, Methodologies, and Comparative Evaluation

  Molecular weight represents one of the most fundamental and critical physicochemical parameters of biomolecules. Across diverse life science disciplines, including protein engineering, antibody drug development, synthetic compound validation, and metabolomics, accurate molecular weight determination serves not only as a prerequisite for structural characterization but also as a cornerstone for quality control and functional investigation. Liquid chromatography–mass spectrometry (LC-MS), characterized ......

• • Which Biopharmaceuticals Can Be Analyzed by High-Resolution Mass Spectrometry for Molecular Weight?

  High-resolution mass spectrometry (HRMS), owing to its exceptional mass accuracy, high sensitivity, and broad measurable mass range, has become an essential analytical tool in biopharmaceutical research and development. Beyond precise molecular weight determination of complex proteins, HRMS enables the resolution of subtle mass differences, providing critical support for structural characterization, impurity profiling, and quality control of biopharmaceutical products. Below, different categories of b......

• • Acetylated Peptide Enrichment Analysis

  Lysine acetylation is a prevalent post-translational modification (PTM) observed in both eukaryotic and prokaryotic organisms. It participates in epigenetic regulation, cell cycle progression, metabolic pathways, and signal transduction. In particular, histone acetylation plays a critical role in chromatin remodeling and transcriptional regulation, where the acetylation states of histones directly influence gene expression. However, compared with PTMs such as phosphorylation and ubiquitination, system......

• • Label-Free Quantitative Glycoproteomics Using DIA-MS

  Glycoproteins participate extensively in key biological processes such as cell recognition, signal transduction, and immune regulation, and dynamic alterations in their glycosylation states are often closely associated with various diseases. Glycoproteomics has therefore emerged as a crucial approach for biomarker discovery and mechanistic studies. To enable systematic quantification of glycosylated proteins, more stringent analytical requirements have been placed on glycoprotein analysis workflows. D......

• • Comprehensive Workflow for Phosphoproteomics Data Processing and Bioinformatic Interpretation

  Phosphoproteomics, a high-throughput mass spectrometry-based analytical approach for characterizing protein phosphorylation, provides a crucial means to interrogate the regulatory roles of phosphorylation in cellular signaling, cell-cycle control, and metabolic regulation. As a dynamic and reversible post-translational modification (PTM), phosphorylation modulates a broad spectrum of biological processes and has become indispensable in mechanistic studies of signal transduction and disease-relevant pa......