Resources

• • Vaccine Development

  Vaccine Development encompasses the entire process from initial antigen discovery to final clinical trials and regulatory approval. Vaccines are designed to prevent infectious diseases by stimulating the immune system to produce specific antibodies, providing protection against future infections. The core of vaccine development lies in identifying and validating antigens capable of inducing effective immune responses. With advancements in proteomics, genomics, and bioinformatics, these technologies ar......

• • Receptor-Ligand Binding Assay

  Receptor-ligand binding assays are among the core techniques for studying molecular interactions and play a crucial role in life sciences and drug development. The binding of receptors and ligands underpins many biological processes, such as cellular signal transduction, metabolic regulation, and immune responses. Receptors can be proteins located on the cell surface or within the cell, while ligands may include small molecules, peptides, nucleic acids, antibodies, or other proteins. By investigating ......

• • Affinity Purification-Mass Spectrometry

  Affinity Purification-Mass Spectrometry (AP-MS) is an integrated method that combines specific protein separation techniques with high-sensitivity molecular detection, widely applied in proteomics research. The principle of this technique involves selectively isolating target proteins and their associated molecules from complex biological samples through affinity purification, followed by identification and quantification using mass spectrometry. Affinity purification relies on high-specificity inte......

• • Functional Proteomics

  Functional proteomics is a scientific discipline dedicated to studying protein functions within cells and organisms, focusing on elucidating their biological roles and dynamic changes under specific conditions. By integrating proteomics technologies with functional analyses, this field investigates protein roles in both physiological and pathological processes, providing valuable insights into life sciences. Unlike traditional proteomics, which primarily emphasizes protein identification and quantif......

• • AP-MS

  Affinity purification-mass spectrometry (AP-MS) integrates affinity purification techniques with mass spectrometry to investigate molecular interactions, the composition of protein complexes, and the dynamic behavior of functional biomolecules. This method leverages the specific binding properties of target molecules, such as proteins, nucleic acids, or small molecules, to isolate them from complex biological samples while simultaneously capturing associated interacting molecules. Subsequent identific......

• • Competitive Ligand Binding Assay

  Competitive ligand binding assay is an experimental technique used to elucidate the binding mechanisms and biological functions of small molecules, drugs, or other ligands by analyzing their competition with biomacromolecules such as proteins, DNA, or RNA. This method is integral to drug discovery, target identification, and mechanistic studies, allowing researchers to characterize ligand binding properties, pinpoint critical binding sites, and refine the activity of lead compounds. The core principle......

• • Ligand Assay

  Ligand assay is a pivotal technique for investigating interactions between biomolecules and their ligands, enabling the determination of critical parameters such as binding mechanisms, kinetic profiles, and affinity. Ligands, which include small molecules, proteins, nucleic acids, or other chemical entities, bind specifically to target molecules. This binding often governs essential biological processes such as enzymatic catalysis, signal transduction, metabolic regulation, and gene expression. By qua......

• • Ligand Binding Assay

  The ligand binding assay is a fundamental methodology used to investigate interactions between biomolecules and ligands, providing critical insights into binding properties and kinetic parameters. Ligands, which may include small molecules, proteins, nucleic acids, or other chemical compounds, often play essential roles in biological processes such as enzymatic catalysis, signal transduction, and molecular transport. By assessing parameters such as binding affinity (e.g., dissociation constant), bindi......

• • Proximity Labeling

  Proximity Labeling is an innovative biological technique designed to investigate spatial protein interactions and molecular relationships within the cellular microenvironment. This approach leverages enzymes capable of catalyzing proximity-specific reactions, such as biotin ligase or peroxidase, to covalently attach distinct markers (e.g., biotin or fluorescent molecules) to molecules in the vicinity of target proteins. These labeled molecules are subsequently identified and quantified using mass spec......

• • Immunoprecipitation Analysis

  Immunoprecipitation analysis is a technique that utilizes the specific binding between antigens and antibodies to isolate and enrich target proteins or their complexes from complex biological samples. By capturing the target protein with a specific antibody and applying elution and detection methods, this technique facilitates the separation and functional analysis of proteins. It is a versatile and widely applied tool in molecular biology, proteomics, and biomedical research, particularly for explori......