Resources
Proteomics Databases
Metabolomics Databases
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• Mass Spectrometry-Based De Novo Protein Sequencing
De novo protein sequencing refers to the process of determining the amino acid sequence of peptides using tandem mass spectrometry (MS/MS) without relying on pre-existing sequence databases. The core principle of de novo protein sequencing is to calculate the mass of amino acid residues in the peptide backbone by measuring the mass difference between fragment ions. The mass-to-charge ratio (m/z) of these ions typically allows for the unique identification of the residue type, thereby determining the......
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• Why Is Protein Sequencing Better Than DNA Sequencing?
Protein sequencing and DNA sequencing are two distinct molecular analysis techniques. DNA sequencing involves the analysis of deoxyribonucleic acid (DNA), the primary carrier of genetic information, which is located in the cell's nucleus. In contrast, protein sequencing focuses on proteins, which are the principal molecules performing diverse functions within the cell. Proteins are encoded by DNA and synthesized through RNA transcription and translation. Common methods for DNA sequencing include San......
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• What Is the Protein Sequence?
A protein sequence, defined as the arrangement of amino acids in a protein molecule, constitutes the primary structure of the protein, also known as its basic structure. This sequence not only determines the protein's three-dimensional conformation but also influences its biological function within the organism. Protein Sequence—Types of Amino Acids Proteins are macromolecules made up of 20 standard amino acids linked by peptide bonds. These amino acids include glycine (Gly), alanine (Ala), valine (......
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• How Is the Protein Sequence Obtained?
Proteins are essential drivers of life processes, performing a wide array of functions, from catalyzing biochemical reactions to constructing cellular structures. Understanding protein structure and function is crucial in fields such as biology, medicine, and biotechnology. The protein sequence, which refers to the amino acid sequence, is a fundamental aspect of understanding both protein function and structure. This article outlines several methods for obtaining protein sequences. Database Search W......
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• What Is the Role of Protein Sequencing?
Protein sequencing, as a key method for deciphering protein structure and function, plays an indispensable role in these processes. In 1954, Sanger successfully sequenced bovine insulin, confirming for the first time the role of amino acids in protein synthesis, thus marking the beginning of the protein sequencing field. In the early 1970s, Sanger developed DNA sequencing technology, laying the foundation for the modification of protein sequences. Proteins are critical macromolecular organic compound......
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• What Are the Main Functions of Olink Proteomics?
Olink proteomics is an advanced biotechnology that enables high-throughput, high-sensitivity, and high-specificity quantitative analysis of specific proteins in biological samples. This is achieved using the innovative Proximity Extension Assay (PEA) technology. Core Technology and Advantages The central technology behind Olink proteomics is the PEA technique, which involves the specific recognition and binding of antibodies to target proteins, followed by the amplification of oligonucleotide se......
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• What Is Protein Normalization Expression in Olink?
The protein normalization expression in Olink is a critical aspect of the Proximity Extension Assay (PEA) platform developed by Olink. The principle of this method involves using two labeled oligonucleotide probes, which, upon binding to a target protein, bring the probes into close proximity, allowing for detection through PCR amplification. This technique is highly sensitive, capable of detecting low-abundance proteins while minimizing non-specific signal interference. The protein normalization expr......
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• What Are the Steps of Edman Degradation?
Edman degradation involves a series of steps to determine the amino acid sequence of a protein or peptide, specifically targeting the N-terminal amino acid. These steps include: 1. Adsorption of the Sample The protein or peptide to be sequenced is adsorbed onto a solid surface, with glass fiber coated with polybutadiene (a cationic polymer) being a commonly used support material. This is the first of the steps of Edman degradation where the sample is prepared for further chemical reactions. 2. Coupl......
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• What Is the Edman Degradation Method?
The Edman degradation method, developed by Pehr Edman in the 1950s, is a technique used for sequencing amino acids in a peptide or protein. This method sequentially removes one amino acid at a time from the N-terminal of the polypeptide chain. The cleaved amino acid is identified using high-performance liquid chromatography (HPLC) or similar techniques. Each cycle reveals one amino acid, and by repeating the process, the complete amino acid sequence can be determined. Analysis Workflow The key steps......
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Protein electrophoresis is a commonly used analysis method for separating and identifying proteins. To overcome the limitations of traditional electrophoresis technology and improve the analysis effect, researchers continue to explore and develop novel electrophoresis techniques in protein band analysis.
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