Resources

Proteomics Databases



Metabolomics Databases

• • Accurate Determination of Protein Molecular Weight by Mass Spectrometry

  Proteins serve as the primary functional molecules in cellular processes, and their molecular weight directly reflects their amino acid sequence and structural integrity. As such, molecular weight is a critical parameter in protein identification, functional prediction, and quality control. In the biopharmaceutical industry, minor variations in protein molecular weight often indicate post-translational modifications (e.g., glycosylation, oxidation) or the presence of degradation products, which are cr......

• • Designing an Efficient Co‑immunoprecipitation (Co‑IP) Protocol

  Co-immunoprecipitation (Co‑IP) is a classical technique for investigating protein–protein interactions, widely applied in the analysis of signaling pathways, target validation, and drug mechanism studies. By enriching target proteins and their associated complexes using specific antibodies, followed by detection via Western blot or mass spectrometry, Co‑IP enables the effective capture of native protein interactions within cells. Despite its seemingly straightforward principle, Co‑IP is technically de......

• • Targeted Metabolomics: Principles and Key Techniques

  Metabolites represent the most immediate biochemical reflection of physiological states in life science research. Unlike genomics, transcriptomics, or proteomics, metabolomics directly captures cellular and systemic responses to internal and external environmental perturbations, making it a vital tool for elucidating disease mechanisms, identifying biomarkers, and exploring drug actions. Among various branches of metabolomics, targeted metabolomics has gained increasing prominence in diverse research ......

• • Mass Spectrometry in Histone Modification Research: Advantages and Challenges

  Post-translational modifications (PTMs) of histones play a central role in regulating chromatin architecture, gene expression, and cell fate decisions. Diverse modification types, including acetylation (Ac), methylation (Me), phosphorylation (P), and ubiquitination (Ub), constitute a complex histone modification code that collectively orchestrates epigenetic regulation. In recent years, the rapid advancement of mass spectrometry (MS) has transformed histone PTM research, enabling comprehensive analysi......

• • Quantitative Methods for Phosphoproteomics

  Quantitative phosphoproteomics encompasses a suite of experimental strategies that utilize mass spectrometry to measure the sites, abundance, and dynamic alterations of protein phosphorylation within biological samples. These approaches enable researchers to monitor phosphorylation changes under varying biological conditions, such as disease states, pharmacological interventions, or signal stimulation. As one of the most prevalent and essential reversible modifications in cellular signaling, protein p......

• • Fundamental Principles of High-Resolution Mass Spectrometry

  High-Resolution Mass Spectrometry (HRMS) is an advanced analytical technique capable of precisely determining ion masses with exceptionally high mass resolution. It is widely employed for the detection of trace components in complex biological samples and finds broad applications in proteomics, metabolomics, drug metabolism studies, and biomarker discovery. The primary advantage of HRMS lies in its ability to accurately resolve ions with minimal mass differences, thereby enabling high-sensitivity and ......

• • Protein Phosphorylation: Mechanisms, Types, and Research Techniques

  Protein phosphorylation is a reversible form of post-translational modification that plays a pivotal role in cellular processes such as signal transduction, metabolic regulation, and cell cycle progression. By covalently attaching phosphate groups to specific amino acid residues, typically serine, threonine, or tyrosine, phosphorylation induces rapid changes in protein structure and function. This mechanism enables cells to swiftly respond to external stimuli. Aberrant phosphorylation states are intim......

• • Using ABPP for Small Molecule Screening and Mechanistic Studies

  In the early stages of drug discovery and mechanism-of-action (MoA) research, traditional high-throughput screening (HTS) approaches can identify active compounds from large chemical libraries. However, they often fail to address a critical question: How does the compound exert its biological effect? This is precisely where Activity-Based Protein Profiling (ABPP), a proteomics technique based on active-site-directed chemical labeling, offers unique advantages. ABPP is a chemical probe-based strategy e......

• • Why ABPP Is Regarded as a Powerful Tool in Functional Proteomics: A Comprehensive Overview of Its Principles and Applications

  Uncovering a Fundamental Challenge: Protein Expression ≠ Protein Activity In life science research and drug development, researchers frequently encounter paradoxical observations: 1. An enzyme shows high expression levels in transcriptomic and proteomic data, yet exhibits no detectable activity during functional validation. 2. Drug treatment results in significant biological effects, even though the expression level of the target protein remains unchanged. 3. Dozens or even hundreds of candidate targe......

• • How to Analyze Subcellular Protein Translocation Using LC-MS?

  Cells are highly dynamic and intricately regulated systems rather than static entities. Under diverse physiological and pathological conditions, proteins often redistribute among distinct subcellular compartments, a process known as subcellular protein translocation. This phenomenon not only reflects alterations in protein functional states but also plays a direct role in essential biological processes such as signal transduction, metabolic regulation, cell cycle control, and apoptosis. Conventional a......