Resources

Proteomics Databases



Metabolomics Databases

• • How to Use GFP Tags for Co-Immunoprecipitation?

  GFP-tag Co-IP using GFP-Trap beads, gentle lysis, wash optimization, and optional LC-MS/MS for interactome discovery.

• • Chemoproteomics: Principles, Workflow, and Applications

  Chemoproteomics uses activity-based probes and LC-MS to map protein–small-molecule interactions, targets, and enzyme activity.

• • Histone Phosphorylation in Epigenetic Regulation

  Definition and roles of histone phosphorylation in transcription, DNA damage response, and mitosis, plus MS detection approaches.

• • How to Analyze Histone Phosphorylation Using Mass Spectrometry

  LC-MS/MS workflow for histone phosphorylation: acid extraction, propionylation, phosphopeptide enrichment, and site localization.

• • Acetylation Site Analysis by Acetyl-Proteomics LC-MS/MS

  Workflow for acetylation site analysis: enrichment, LC-MS/MS, and site localization by acetyl-proteomics.

• • Exosome Proteomics: Overview and Workflow

  Overview of exosome proteomics: EV isolation, LC-MS/MS quantification, and biomarker applications.

• • DDA in Proteomics: Data-Dependent Acquisition Explained

  Explanation of DDA in proteomics: MS1 survey, Top N MS2, advantages, limitations, and synergy with DIA workflows.

• • How to Optimize DDA Parameters for High-Quality Protein Identification

  Optimize DDA MS1/MS2 parameters, dynamic exclusion, LC gradients, and sample prep for deeper, reproducible protein identification in LC-MS/MS proteomics.

• • Histone Phosphorylation Detection: Sample Preparation Methods That Improve Sensitivity

  Sample preparation methods that improve histone phosphorylation detection: phosphatase inhibition, acid extraction, propionylation, fractionation, and enrichment.

• • LC-MS/MS-Based Exosome Proteomics Workflow: Isolation, Digestion, Acquisition, and Analysis

  A step-by-step LC-MS/MS exosome proteomics workflow from isolation and digestion through DDA/DIA/PRM acquisition and bioinformatics.