Resources

Proteomics Databases



Metabolomics Databases

• • How ABPP Supports High-Throughput Compound Screening: Target Engagement, Off-Target Profiling, and Workflow Design

  A practical guide to how activity-based protein profiling (ABPP) supports high-throughput compound screening through competitive labeling, functional target validation, off-target profiling, and LC-MS/MS readouts.

• • SDS-PAGE Protein Detection Methods: Western Blot, Mass Spectrometry, and 2D-PAGE Beyond Gel Electrophoresis

  A practical guide to protein detection methods used with SDS-PAGE, including Western blot, mass spectrometry, 2D-PAGE, in-gel workflows, method selection, and common limitations.

• • Efficient and Accurate Protein Sequencing Methods: Mass Spectrometry, Edman Degradation, and Workflow Selection

  A practical guide to efficient and accurate protein sequencing methods, comparing mass spectrometry, Edman degradation, de novo interpretation, terminal analysis, and gene-based sequence inference for research and biopharma workflows.

• • Glycosylation Site Analysis in Protein Function Studies: LC-MS/MS Workflows, Site Occupancy, and Biological Interpretation

  A practical guide to glycosylation site analysis in protein function studies, covering N- and O-glycosylation, LC-MS/MS workflows, enrichment, site occupancy, glycoform interpretation, and disease or biopharma applications.

• • Cell Surface Proteomics Analysis: Enrichment Strategies, Membrane Protein Identification, and Biomarker Discovery

  A practical guide to cell surface proteomics analysis, covering biotinylation, shaving, membrane protein enrichment, DDA and DIA workflows, localization filtering, and biomarker discovery.

• • LC-MS/MS Protein Sequencing: Workflow, De Novo Interpretation, and Proteomics Applications

  A practical guide to LC-MS/MS protein sequencing, covering sample preparation, peptide fragmentation, database search, de novo interpretation, quantitative readouts, and applications in proteomics research.

• • Mass Spectrometry in Subcellular Proteomics: Organelle Fractionation, Localization, and Quantitative Analysis

  A practical guide to how mass spectrometry works in subcellular proteomics, covering organelle fractionation, LC-MS/MS workflows, protein localization, quantitative strategies, contamination control, and spatial proteomics applications.

• • TMT-Based Quantitative Proteomics: Multiplexing, MS3 Accuracy, and Workflow Design

  A practical guide to TMT-based quantitative proteomics, covering tandem mass tags, reporter ions, MS2 vs MS3 quantification, multiplex design, fractionation, and method selection for large comparative studies.

• • iTRAQ LC-MS/MS Quantitative Proteomics Workflow: Multiplexing, Accuracy, and Method Selection

  A practical guide to the iTRAQ LC-MS/MS workflow for quantitative proteomics, covering reporter ions, multiplexing, sample preparation, fractionation, quantitative accuracy, low-abundance detection, and method selection.

• • Co-IP for Low-Expression Proteins: Enrichment Strategies, IP-MS Sensitivity, and Experimental Troubleshooting

  A practical guide to performing co-immunoprecipitation for low-expression proteins, covering antibody choice, gentle lysis, cross-linking, washing, IP-MS sensitivity, contaminant control, and interaction validation.