Resources

Proteomics Databases



Metabolomics Databases

• • How to Select AQUA Peptides for Quantitative Proteomics

  Learn how to select AQUA peptides by defining targets, choosing proteotypic surrogates, designing labels, planning spike levels, and validating heavy-light performance in matrix.

• • How to Plan a Targeted Proteomics Validation Study

  Learn how to plan a targeted proteomics validation study, from target panel selection and matrix review to MRM or PRM assay development, pilot testing, and QC reporting.

• • How to Design an Absolute Protein Quantification Assay

  Learn how to design an absolute protein quantification assay, from reporting units and AQUA standard strategy to calibration, MRM or PRM optimization, and QC reporting.

• • How to Build a Parallel Reaction Monitoring Assay

  Learn how to build a parallel reaction monitoring assay by selecting proteotypic peptides, optimizing isolation windows, choosing fragment ions, and validating targeted quantitation.

• • Budgeting a Targeted Mass Spectrometry Project

  Learn what drives targeted mass spectrometry project budget, including MRM or PRM platform choice, panel size, matrix complexity, cohort size, and reporting before requesting a quote.

• • AQUA Peptides vs External Standards for Quantification

  Compare AQUA peptides and external standards for absolute quantification by matrix complexity, precision needs, cost, and reporting goals in targeted proteomics.

• • AQUA Peptides: Principles for Absolute Quantification

  Learn how AQUA peptides enable absolute quantification through stable isotope labeling, isotope dilution, calibration design, and targeted MRM or PRM acquisition.

• • Scoping an AQUA Peptide Project: Synthesis, Spike Design, and Validation Deliverables

  Plan an AQUA peptide project with clear synthesis specs, spike design, matrix validation, and reporting deliverables. Avoid standard rework and scope mismatches.

• • AQUA Peptide or Other Internal Standards? Comparing Isotope Dilution Routes for Targeted Quantitation

  Compare AQUA peptides with SILAC, unlabeled spikes, and external calibration for targeted quantitation. Match the standard strategy to your reporting goal.

• • AQUA Peptide Not Performing? Troubleshooting Standard Purity, Spike Level, and Matrix Recovery

  Weak heavy-peptide signal or unstable ratios in AQUA peptide assays? Learn how to troubleshoot standard purity, spike level, storage, and matrix recovery.