Resources

Proteomics Databases



Metabolomics Databases

• • SPR vs. BLI vs. ELISA: Comparing the Advantages and Limitations of Three Antibody Assay Techniques

  The investigation of antibody-antigen interactions represents a central component of life sciences, drug development, and immunodiagnostics. The selection of an appropriate analytical technique is critical for ensuring the accuracy of experimental results and the reliability of data interpretation. Surface Plasmon Resonance (SPR), Bio-Layer Interferometry (BLI), and Enzyme-Linked Immunosorbent Assay (ELISA) are currently among the most widely employed antibody detection methods. Each technique has dis......

• • How to Conduct Research on Mitochondrial Protein-Protein Interaction Networks?

  Mitochondria are traditionally regarded as the "powerhouse" of the cell; however, their roles extend far beyond ATP production. Recent studies indicate that mitochondria are also involved in critical cellular processes, including apoptosis, metabolic regulation, immune signaling, and oxidative stress responses. Mitochondrial functions rely on the coordinated interactions among thousands of proteins, and the protein-protein interaction (PPI) networks formed by these proteins provide a critical framewor......

• • How to Optimize LC-MS/MS Workflow for FFPE Proteomics?

  FFPE (Formalin-Fixed Paraffin-Embedded) samples represent the most stable and information-rich tissue resources in clinical research. Pathology archives contain extensive case histories and follow-up information, making them especially suitable for tumor subtyping, prognostic biomarker identification, and retrospective cohort validation. Nevertheless, many laboratories observe that, even when using the same instrument, identical gradients, and the same search parameters, the depth of protein identific......

• • How to Measure the Extinction Coefficient for Proteins and Formulations?

  UV-Vis guidance for extinction coefficients (molar absorptivity), A280 protein workflows, Beer–Lambert pitfalls, and orthogonal quantification options in biopharma.

• • PRM-Based Targeted Quantification of Plasma Samples

  Plasma is one of the most clinically valuable sample types in proteomics research because of its broad availability, ease of collection, and rich representation of systemic biological information. However, the plasma proteome spans a dynamic concentration range of up to 10 orders of magnitude, and high-abundance proteins, such as albumin and immunoglobulins, account for more than 90% of the total protein mass. As a result, low-abundance proteins, particularly potential disease biomarkers, are difficul......

• • From Principles to Applications: A Comprehensive Overview of PRM Technology

  In proteomics research, achieving precise, robust, and reproducible quantification of specific proteins or peptides has long been a central objective. Parallel Reaction Monitoring (PRM), a targeted mass spectrometry approach characterized by high specificity, high sensitivity, and high throughput, has increasingly emerged as an attractive alternative to conventional SRM/MRM methods and has been widely applied in cutting-edge areas such as biomarker validation, clinical research, and drug development. ......

• • Mass Spectrometry‑Based Quantitative Proteomics Using SILAC

  With the advancement of large-scale mass spectrometry, proteomics research has gradually shifted from identification-focused analysis to quantitative analysis. Compared with conventional protein detection methods such as Western blotting or ELISA, mass spectrometry-based quantitative proteomics offers high throughput, high accuracy, and broad proteome coverage. Among the available quantitative strategies, SILAC has been widely applied in cell-based studies because of its high precision, low technical ......

• • Advantages and Disadvantages of SILAC

  Quantitative analysis of protein expression is a fundamental objective in modern biological research and is widely applied to the elucidation of signal transduction mechanisms, identification of drug targets, and screening of disease biomarkers. With the continued development of mass spectrometry, a variety of quantitative strategies have been introduced. Among them, SILAC (Stable Isotope Labeling by Amino acids in Cell culture) has become one of the mainstream approaches for protein quantification in......

• • How to Identify Lactylation Sites on Histones?

  Histone lactylation, as a newly identified epigenetic modification, has garnered increasing attention. This modification demonstrates that lactate, a metabolic intermediate, is not merely a byproduct of energy metabolism but can also regulate gene expression by modifying lysine residues on histones. Compared with classical modifications such as acetylation and methylation, lactylation establishes a direct link between cellular metabolic states and chromatin dynamics, exhibiting unique biological signi......

• • How Does the ER Proteome Contribute to Secretory Pathway Regulation?

  In eukaryotic cells, the protein secretory pathway is a highly organized transport system that involves multiple organelles, including the endoplasmic reticulum (ER), the Golgi apparatus, vesicles, and the plasma membrane. The ER proteome, serving as the primary entry site of this pathway, is responsible not only for protein synthesis, folding, and initial post-translational modification but also for directly influencing secretion efficiency and protein fate through sophisticated quality control and s......