Resources

Proteomics Databases



Metabolomics Databases

• • Tailored Host Cell Protein Profiling Solutions for Biopharmaceutical Projects

  With the rapid advancement of the biopharmaceutical industry, high-value therapeutics such as recombinant proteins, monoclonal antibodies (mAbs), and fusion proteins have become increasingly important in clinical applications. During the research, development, and production of these biologics, host cell proteins (HCPs), as impurities derived from the expression system, may remain in trace amounts in the final drug product after purification. Although present at very low levels, HCP immunogenicity, en......

• • Mass Spectrometry-Based HCP Identification in Antibody Drug Manufacturing

  With the rapid advancement of biopharmaceutical technologies, monoclonal antibodies (mAbs) have emerged as one of the most commercially valuable therapeutic biologics. However, during large-scale production, host cell proteins (HCPs), as process-related impurities, represent critical factors influencing product quality, safety, and consistency. HCPs primarily originate from upstream cell culture expression systems, such as CHO cells, and may partially persist in the final product following cell lysis,......

• • Workflow for Mass Spectrometry-Based Untargeted Metabolomics

  In systems biology research, metabolomics acts as a critical bridge linking the genome, transcriptome, and proteome, and has increasingly become a key tool for elucidating biological processes. Specifically, untargeted metabolomics is widely employed in studies of disease mechanisms, biomarker discovery, and drug development due to its broad metabolite coverage and high discovery potential. Mass spectrometry, with its high sensitivity and resolution, serves as the central platform for untargeted metab......

• • Molecular Mechanisms and Labeling Strategies for Protein Lactylation Modification

  In recent years, with the rapid integration of metabolomics and epigenetics, protein lactylation, an emerging lysine post-translational modification (PTM), has attracted increasing attention from the scientific community. Since its initial discovery reported in Nature in 2019, lactylation has been demonstrated to be widely present in both histone and non-histone proteins, where it plays critical roles in gene expression regulation and cellular metabolic reprogramming. What Is Protein Lactylation? Pro......

• • How to Perform Glycan Structural Analysis for Antibody Drugs?

  Monoclonal antibody therapeutics (mAbs), owing to their high specificity and therapeutic potential, have become central products in modern biopharmaceuticals. The bioactivity, half-life, and immunogenicity of antibodies are often significantly modulated by glycosylation. Variations in glycan structures can influence both therapeutic efficacy and safety. Therefore, comprehensive glycan characterization is a critical component in antibody research, development, manufacturing, and quality control. Overv......

• • Top Methods for N‑Glycosylation and O‑Glycosylation Profiling

  In protein post-translational modification (PTM) research, glycosylation represents one of the most structurally complex and widely distributed types of modification. Glycosylation not only influences protein folding, stability, and biological activity but also plays essential roles in cellular signaling, immune regulation, and disease initiation and progression. Depending on the glycosylation site, glycosylation is primarily classified into N-glycosylation and O-glycosylation. In studies of protein f......

• • What Is the Relationship Between Histone Acetylation and Gene Activation?

  Histone acetylation is one of the most classical and representative post-translational modifications in epigenetic regulation and exhibits a strong positive correlation with gene expression activation. In eukaryotic cells, DNA does not exist in a naked form but is wrapped around histone octamers to form nucleosome structures. Histone acetylation promotes transcription initiation and elongation at multiple levels by altering chromatin architecture and modulating the recruitment efficiency of the transc......

• • Targeted Metabolomics Analysis Process

  Targeted metabolomics has emerged as a core approach for investigating dynamic changes in biological systems, particularly in precision medicine, drug development, and mechanistic studies. Owing to its high sensitivity, quantitative accuracy, and reproducibility, targeted metabolomics is widely used for quantitative analyses and mechanistic validation. This article provides a systematic overview of the complete workflow of targeted metabolomics, aiming to help researchers understand critical technical......

• • Untargeted Metabolomics: Sample Collection, Processing, and QC

  With the rapid advancement of systems biology research, untargeted metabolomics has become a crucial tool for investigating dynamic changes in biological systems. Unlike targeted approaches, untargeted metabolomics aims to comprehensively capture metabolite profiles within samples, enabling biomarker discovery, metabolic pathway elucidation, and studies of disease mechanisms. However, the extensive diversity and varying physicochemical properties of metabolites mean that every step in the experimental......

• • TMT-Based Quantitative Proteomics in Cancer Research

  Cancer is a highly heterogeneous and complex disease, involving biological processes at multiple levels, including gene mutations, regulation of protein expression, and dysregulation of signaling pathways. Although high-throughput transcriptomic techniques such as RNA-Seq have been extensively applied in cancer research, transcriptional profiles do not fully reflect protein expression or functional states. TMT-based quantitative proteomics, a relative quantification approach utilizing isobaric mass ta......