Resources

Proteomics Databases



Metabolomics Databases

• • SILAC-Based Mass Spectrometry

  SILAC-based mass spectrometry is a powerful tool for quantitative proteomics. SILAC involves the endogenous labeling of proteins by introducing stable isotope-labeled amino acids, such as ^13C or ^15N labeled lysine and arginine, into cell cultures. This results in proteins that exhibit specific isotope labels, which can be detected as mass differences in mass spectrometry analysis. SILAC-based mass spectrometry is primarily used for the relative quantification of proteins, playing a crucial role in i......

• • Shotgun Protein Identification

  Shotgun protein identification is a proteomics technique fundamentally reliant on mass spectrometry. It provides profound biological insights by thoroughly identifying and analyzing complex protein mixtures. Unlike traditional protein identification techniques, Shotgun does not depend on the prior separation and purification of proteins. Instead, it directly examines peptide mixtures generated from enzymatic digestion of protein samples. The methodology is characterized by its high throughput and sens......

• • Quantitative and Qualitative Analysis of Proteins

  Quantitative and qualitative analysis of proteins is fundamental to understanding biological functions and mechanisms. As key regulators of cellular processes, proteins undergo changes in abundance and structural modifications that directly influence physiological and pathological states. Quantitative analysis primarily focuses on determining protein concentration, enabling researchers to investigate expression variations under different conditions. For instance, comparing protein concentrations betwe......

• • Quantitative Analysis of Newly Synthesized Proteins

  Quantitative analysis of newly synthesized proteins is a powerful approach for measuring and evaluating the expression dynamics of nascent proteins within cells. This technique is essential in proteomics research, offering critical insights into protein synthesis rates, turnover mechanisms, and cellular regulatory pathways. By quantifying newly synthesized proteins, researchers can dissect the processes of protein production and degradation, elucidating cellular responses under various physiological a......

• • Qualitative Mass Spectrometry

  Qualitative mass spectrometry is an advanced analytical method used for the qualitative characterization of proteins within samples by employing mass spectrometry. As a robust tool, mass spectrometry allows for the identification and characterization of proteins present in complex mixtures. By measuring the mass-to-charge ratio (m/z) of protein molecules and integrating bioinformatics analysis, this approach can elucidate both the protein composition and structural details within a sample. The scope o......

• • Qualitative Determination of Proteins

  Qualitative determination of proteins refers to the process of identifying and characterizing proteins within a sample, playing a fundamental role in proteomics research. This analytical approach is essential for understanding biological functions and molecular mechanisms. In basic research, it aids in elucidating cellular structures and regulatory pathways, providing critical insights into disease mechanisms. Beyond fundamental studies, qualitative determination of proteins has extensive applications......

• • PTM Mass Spec

  PTM mass spec is an advanced technique used to examine the modifications proteins undergo after translation. PTMs are chemical changes occurring on specific amino acids during or after protein synthesis, through enzymatic or non-enzymatic processes. These modifications are vital for regulating protein function, stability, and cell signaling. PTMs include phosphorylation, acetylation, methylation, glycosylation, ubiquitination, and carboxylation, among others. By enhancing chemical diversity, PTMs modu......

• • Proteomics: Top-Down & Bottom-Up

  Proteomics top-down & bottom-up are two fundamental strategies in modern proteomics research and are widely applied in mass spectrometry-based analyses. Top-down proteomics directly analyzes intact protein molecules to determine their sequences and post-translational modifications (PTMs). In contrast, bottom-up proteomics involves enzymatic digestion of proteins into peptides, which are then analyzed via mass spectrometry to reconstruct protein sequences. This approach is particularly suitable for hig......

• • O-Link Proteomics

  O-Link Proteomics is a cutting-edge research methodology leveraging proximity extension assay (PEA) technology to conduct high-throughput and precise measurements of protein dynamics in biological processes. Developed by Olink, this PEA platform allows for the simultaneous quantification of multiple protein expressions. A hallmark of O-Link Proteomics is its high sensitivity and specificity. Traditional proteomics approaches often require large sample volumes and struggle with low detection limits and......

• • 6 Essential Native MS Strategies to Enhance Your Data Quality

  Native MS serves as a powerful tool for in situ characterization of complex biological assemblies, such as protein complexes and nucleic acid-protein interactions, by preserving their native conformation and non-covalent interactions. However, the sensitivity and reliability of native MS data heavily depend on the optimization of experimental conditions and analytical strategies. This article outlines 6 critical strategies covering the entire workflow—from sample preparation and instrumental settings ......