Resources

• • Tandem Mass Spectrometry in Proteomics Research

  Tandem mass spectrometry (TMT) is a quantitative analysis technique widely used in proteomics research. TMT enables the relative quantification of multiple samples in mass spectrometry analysis through chemical labeling. This technique uses isotopically labeled chemical reagents that react with peptides in the sample, imparting unique mass tags to each sample. During the mass spectrometry process, these tags generate distinct fragment ion signals, allowing for direct comparison of protein relative ab......

• • Multiple Reaction Monitoring

  Multiple reaction monitoring (MRM) is a highly sensitive and specific analytical technique based on mass spectrometry, widely used for targeted analysis of complex biological samples. MRM works by selecting specific ion pairs-combinations of parent ions and product ions—to achieve both quantitative and qualitative analysis of target substances. Compared to traditional mass spectrometry, MRM provides significant advantages in terms of sensitivity and specificity, especially for detecting target analyt......

• • SILAC

  Stable isotope labeling by cell culture (SILAC) is a technique used to study proteomics, metabolic pathways, and cellular biological processes. SILAC involves labeling cellular proteins with amino acids containing stable isotopes during cell culture, allowing for the distinction of labeled proteins from unlabeled ones in mass spectrometry analysis. This method provides a powerful tool for researchers to quantify proteins, examine protein-protein interactions, and investigate functional changes, with ......

• • TMT Technology in Biomedical and Environmental Research

  Tandem Mass Tag (TMT) is a chemical labeling method designed for simultaneous analysis of multiple samples by attaching isotope-labeled tags to proteins from different sources. This approach is widely employed in biomedical research for both relative and absolute protein quantification. The principle of TMT involves introducing isotopic tags onto peptides, which produce distinct ion signals during mass spectrometry analysis. By comparing the intensity of these signals, researchers can precisely deter......

• • Quantitative Protein Profiling

  Quantitative protein profiling integrates mass spectrometry technology with bioinformatics analysis to accurately measure the abundance and changes in protein levels within samples. This technique provides researchers with detailed insights into protein expression in biological samples, which is crucial for understanding cellular processes, disease mechanisms, and drug development. In basic research, quantitative protein profiling enables scientists to uncover dynamic alterations in protein networks ......

• • Cyclic Peptide Synthesis

  Cyclic peptide synthesis refers to the chemical or biotechnological processes used to create peptides with a cyclic structure. These cyclic peptides are structurally diverse and exhibit notable biological activity, making them highly applicable in fields such as drug development, agriculture, and biochemical research. For instance, cyclosporine, an important immunosuppressant, is widely used in organ transplants to prevent rejection, while cucurbitacin has demonstrated potential as an anticancer agen......

• • Solid Phase Polypeptide Synthesis

  Solid phase polypeptide synthesis (SPPS) is a chemical synthesis technique used to construct polypeptides with specific sequences. First introduced by Robert Bruce Merrifield in the 1960s, this method revolutionized traditional liquid-phase synthesis by fixing the initial amino acid onto an insoluble solid support (usually resin) and adding subsequent amino acids step by step. In each synthesis cycle, unreacted reagents and by-products are removed through simple washing, thus enhancing the ease and e......

• • Solid Phase Peptide Synthesis

  Solid phase peptide synthesis (SPPS) is a widely used chemical method for the stepwise synthesis of peptides on an insoluble solid support. First introduced by Robert Bruce Merrifield in 1963, solid phase peptide synthesis (SPPS) has become a fundamental technique in biochemistry and biotechnology. The process involves sequentially adding amino acids to a solid phase resin, greatly enhancing both the efficiency and purity of peptide synthesis. SPPS has broad applications, including drug development, ......

• • Proximity Extension Assay PEA Technology

  Proximity extension assay PEA technology is a highly sensitive and specific molecular analysis method used to detect and quantify target proteins in proteomics. This technique utilizes a pair of specific antibodies that bind to the target protein, followed by DNA sequence ligation and amplification, enabling ultra-high sensitivity and specificity in protein detection. The core principle of proximity extension assay PEA technology relies on a phenomenon called the "proximity effect," whereby the DNA s......

• • Quantitative Proteomics in Disease and Drug Discovery

  Quantitative proteomics is an advanced technique designed to accurately measure and compare protein expression levels across different samples. Unlike traditional proteomics methods, quantitative proteomics not only identifies proteins but also provides detailed information on their relative or absolute abundances under various biological conditions. One of the primary advantages of this technique is its ability to capture dynamic changes in protein levels, which often reflect the physiological or pat......