Resources

Proteomics Databases



Metabolomics Databases

• • Label-Free Versus Label-Based Phosphoproteomics: Selection Strategies and Comparative Advantages

  Phosphorylation is a critical post-translational modification (PTM) that plays central roles in signal transduction, cell cycle regulation, and disease development. To systematically characterize the dynamic phosphorylation landscape in cells, quantitative phosphoproteomics has emerged as an essential tool in modern life science research. Among quantitative strategies, label-based and label-free approaches represent the two principal methodologies. Overview of Quantitative Phosphoproteomics Phosphopr......

• • What Enzymes Regulate Histone Propionylation?

  Histone propionylation (HPr) is a newly recognized short-chain fatty acid-derived histone modification that plays an important role in regulating chromatin architecture and gene expression. Unlike conventional acetylation, HPr involves the addition of a propionyl group (C3 chain) to lysine residues and can serve as a more sensitive indicator of intracellular metabolic states, particularly dynamic fluctuations in propionyl-CoA. This modification has demonstrated distinct regulatory potential in hepatic......

• • What Is Histone Monoubiquitination?

  Within the nucleus, DNA does not exist as naked DNA but is tightly wrapped around histones to form chromatin structures. Histones serve not only as DNA-packaging proteins but also as critical regulators of gene expression, DNA repair, and chromosomal stability. Their functions are modulated by multiple reversible post-translational modifications (PTMs), among which monoubiquitination represents an important and distinct regulatory modification. In recent years, studies on histone monoubiquitination ha......

• • How to Detect Histone Succinylation?

  Histone succinylation (Ksucc) is a novel and important epigenetic modification that plays critical roles in chromatin remodeling, transcriptional regulation, metabolic control, and disease-associated biological processes. Owing to its low abundance and rapid dynamic changes, the detection and quantification of succinylation have become essential components of molecular biology and proteomics research. Scientific Basis of Histone Succinylation Detection 1. Chemical Characteristics Histone succinylatio......

• • How to Perform Histone Kbu Proteomics Analysis?

  Histone post-translational modifications (PTMs) are fundamental regulatory mechanisms governing gene expression and chromatin architecture. Among these, lysine butyrylation (Kbu) has emerged as a novel modification that has attracted increasing attention in recent years. Kbu is implicated not only in energy metabolism and cell fate regulation but also in diverse pathological conditions. However, owing to its low abundance and highly dynamic nature, conventional analytical approaches are insufficient f......

• • What Is Histone Succinylation Analysis?

  Histones are essential components of chromatin that not only contribute to DNA packaging and structural stability but also regulate gene expression through a wide range of reversible post-translational modifications (PTMs). In recent years, histone succinylation (Ksucc) has emerged as a novel PTM of significant interest because of its potential roles in cellular metabolism, epigenetic regulation, and disease development. Consequently, histone succinylation has become an important focus in life science......

• • How to Study Histone Ubiquitination by LC-MS/MS?

  Histone ubiquitination is a critical post-translational modification (PTM) in which ubiquitin (Ub) is covalently attached to lysine residues on histones, thereby regulating DNA damage repair, transcriptional activity, and chromatin remodeling. Owing to its important roles in cancer, neurodegenerative disorders, and other diseases, precise identification of histone ubiquitination sites has become a major focus in proteomics research. With its high sensitivity and high-throughput capability, LC-MS/MS (l......

• • How to Prepare Samples for Histone Ubiquitination Analysis?

  Histone ubiquitination is a critical post-translational modification that plays essential roles in gene expression regulation, DNA repair, cell cycle control, and cancer progression. High-quality sample preparation is fundamental to obtaining reliable experimental data, as it helps preserve low-abundance ubiquitination signals while improving the accuracy and reproducibility of mass spectrometry and immunoassay-based analyses. Principles of Sample Selection and Processing Histone ubiquitination studi......

• • What Is C-Terminal Protein Sequencing?

  In protein research, the C-terminus (carboxyl terminus), in addition to the N-terminus (amino terminus), contains important biological information. C-terminal protein sequencing is an analytical approach dedicated to characterizing the amino acid sequence at the protein carboxyl terminus. This technique provides critical insights into protein maturation, post-translational modifications (PTMs), degradation pathways, and functional regulation. Concept of C-Terminal Protein Sequencing Proteins are line......

• • What Is N-Terminal Protein Sequencing?

  In life science research, proteins are not only the primary functional molecules within cells but also critical targets for disease investigation and drug development. To achieve a comprehensive understanding of protein structure and function, researchers have developed a variety of analytical techniques, among which N-terminal protein sequencing represents a fundamental and highly important approach. This technique enables precise characterization of the amino acid sequence at the protein N-terminus,......