Resources

Proteomics Databases



Metabolomics Databases

• • Classification of Protein Glycosylation: An Overview

  Protein glycosylation is one of the most common and structurally complex post-translational modifications (PTMs), widely distributed in eukaryotes and also present in certain prokaryotes. It plays critical roles in regulating protein folding, stability, and subcellular localization, and is extensively involved in diverse biological processes such as cell recognition, signal transduction, and immune regulation. Under pathological conditions, including cancer, autoimmune diseases, and metabolic syndrome......

• • How to Perform Functional Annotation of Acylated Proteins and Analyze Their Associated Signaling Pathways?

  Functional annotation and signaling pathway analysis of acylated proteins are essential for understanding their roles in cellular processes, metabolic regulation, and disease mechanisms. What Are Acylated Proteins? Why Should They Be Studied? Acylation represents an important class of post-translational protein modifications, with common types including: Acetylation Propionylation Butyrylation Succinylation Malonylation Palmitoylation, among others. These modifications typically occur o......

• • How to Evaluate the Quality of Custom Synthetic Peptides?

  In experimental applications such as antibody generation, protein–protein interaction studies, mass spectrometry–based analysis, and vaccine development, the quality of custom synthetic peptides directly determines the reliability and reproducibility of experimental outcomes. With the expanding use of peptides in functional studies, molecular targeting, and novel drug discovery, increasing attention has been devoted to critical parameters including purity, sequence accuracy, and stability. In particul......

• • How Can Quantitative Proteomics Be Used to Investigate Differential Acylation Under Different Conditions?

  In the post-genomic era, post-translational modifications (PTMs) have attracted increasing attention as critical regulatory mechanisms underlying protein functional diversity. Among these, acylation, particularly lysine acetylation, propionylation, butyrylation, and malonylation, represents an important class of lysine modifications that are extensively involved in the regulation of key biological processes, including metabolism, signal transduction, and chromatin remodeling. However, these modificati......

• • Membrane Proteomics for Accelerating Drug Target Discovery

  Membrane proteins play a critical role in cellular physiology and signal transduction and represent one of the most important classes of therapeutic targets in contemporary drug development. However, systematic studies of membrane proteins have long been hindered by their hydrophobic nature, low abundance, and structural complexity. With the advancement of membrane proteomics technologies, researchers can now systematically and in a high-throughput manner investigate the functional roles of membrane p......

• • TMT vs Label-Free Quantitative Proteomics

  Proteomics, as a pivotal tool for elucidating biological mechanisms, is transitioning from a discovery-driven approach to a phase of precise quantification. Among large-scale quantitative strategies, Tandem Mass Tag (TMT) labeling and Label-Free Quantification (LFQ) are the most widely adopted. Although both rely on high-resolution mass spectrometry platforms, they exhibit fundamental differences in experimental design, data quality, and suitable application scenarios. What is TMT-Based Quantificatio......

• • Which Technologies Are Most Suitable for Spatial Proteomics Data Acquisition?

  Spatial proteomics has emerged as a rapidly advancing frontier in life sciences, aiming to decipher the spatial distribution and dynamic alterations of proteins within tissues or cellular microenvironments. To fulfill this objective, data acquisition technologies must integrate high spatial resolution, robust protein detection throughput, and precise quantification. Currently, mainstream spatial proteomic acquisition approaches fall into two broad categories: imaging-based techniques and mass spectrom......

• • An Overview of Protein–Protein Interaction Analysis Techniques

  Protein–protein interactions (PPIs) are fundamental to virtually all cellular processes. From signal transduction and metabolic regulation to structural maintenance and immune responses, cellular functionality critically depends on the specificity and dynamic regulation of protein associations. Comprehensive analysis of PPIs not only enhances our understanding of molecular mechanisms under physiological conditions but also identifies key regulatory nodes involved in disease onset and progression. Eluc......

• • What Is the Difference Between Spatial Proteomics and Spatial Transcriptomics?

  Spatial proteomics and spatial transcriptomics are two fundamental technologies in the field of spatial omics. Both aim to characterize the molecular distribution within cells and tissues in a spatially resolved manner, but they differ in the types of biomolecules analyzed and their technical strategies. Definitions and Research Objects     In summary: Spatial proteomics reveals what cells are doing at specific locations. Spatial transcriptomics reveals what cells are preparing to do at specific......

• • Differences Between Peripheral and Integral Membrane Proteins and Methods for Their Identification

  Membrane proteins and integral membrane proteins are critical components of the cellular membrane system, playing essential roles in signal transduction, molecular transport, and cell recognition. Although both types of proteins are associated with biological membranes, they differ significantly in structural characteristics, membrane association mechanisms, and functional properties, resulting in distinct experimental identification strategies. Differences Between Membrane Proteins and Integral Memb......