Resources

Proteomics Databases



Metabolomics Databases

• • Applications of 4D Proteomics in Tumor Biomarker Discovery

  Early cancer diagnosis and personalized treatment have become central challenges in precision medicine. The identification of tumor biomarkers with high specificity and sensitivity is critical for advancing early screening and therapeutic monitoring. Although proteomics has laid an important foundation for tumor biomarker discovery, it still faces limitations in sensitivity, proteome coverage, and data depth. Four-dimensional proteomics (4D proteomics) offers a promising solution to these challenges. ......

• • What Workflows Are Used in Endoplasmic Reticulum Proteomics?

  The endoplasmic reticulum (ER) is a major membrane-bound organelle that is involved in essential biological processes, including protein folding, lipid synthesis, calcium storage, and cellular stress responses. In proteomics research, characterizing the ER proteome and its dynamic changes is important for understanding cellular homeostasis and disease-related mechanisms, including ER stress and tumorigenesis. However, because of the structural complexity of the ER and its extensive interactions with o......

• • Main Types of Protein Glycosylation: N-Linked, O-Linked, and GPI Anchors Explained

  A practical guide to the main types of protein glycosylation, N-linked, O-linked, and GPI anchors, including biosynthesis, consensus motifs, biological roles, and mass spectrometry analysis approaches.

• • Cell Sequencing: Single-Cell and Population-Level Workflows, Applications, and Data Analysis

  A practical guide to cell sequencing, covering single-cell sequencing, population cell sequencing, sample preparation, isolation methods, WGA, WTA, NGS library construction, data analysis, and biomedical applications.

• • How to Determine Amino Acids from a Mass Spectrum and Locate Modification Sites?

  A practical guide to interpreting MS/MS spectra for amino acid assignment, residue mass differences, post-translational modification detection, and modification site localization in proteomics.

• • Activity-Based Protein Profiling in Drug Discovery: Workflow, Applications, and Method Selection

  A practical guide to activity-based protein profiling (ABPP) for drug discovery, covering activity probes, LC-MS/MS, target engagement, competitive profiling, and chemical proteomics workflows.

• • Bottom-Up Proteomics in Single-Cell Proteomics: Challenges, Workflow, and Method Selection

  A practical guide to bottom-up single-cell proteomics, covering sample preparation, TMT multiplexing, carrier boost, LC-MS/MS, DIA vs DDA, missing values, and emerging opportunities.

• • Histone Butyrylation (Kbu): Butyrate Metabolism, Epigenetic Regulation, and LC-MS/MS Detection

  A practical guide to histone butyrylation (Kbu), including butyryl-CoA formation, links to butyrate metabolism, lysine acylation biology, writers and erasers, disease relevance, and LC-MS/MS detection.

• • LC-MS Histone Modification Detection: PTM Signatures, Workflows, and Quantification

  A practical guide to LC-MS histone modification detection, covering histone extraction, peptide preparation, enrichment, acetylation, methylation, phosphorylation, mass shifts, site localization, and quantitative PTM analysis.

• • DIA vs DDA Proteomics: Acquisition Strategy, Quantification, and Method Selection

  A practical comparison of DIA and DDA proteomics, including precursor selection, MS/MS complexity, reproducibility, missing values, spectral libraries, quantitative accuracy, and application scenarios.