Resources

• • SDS PAGE Protein Purification

  SDS page protein purification is a widely used technique in biochemistry and molecular biology for separating and analyzing individual proteins from complex mixtures. SDS page, or Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, relies on the migration of proteins in an electric field for their separation. SDS is an anionic detergent that binds non-covalently to proteins, imparting a negative charge. Additionally, SDS denatures proteins by disrupting their secondary and tertiary structures, ......

• • Protein Interaction Network Analysis

  Protein interaction network analysis is a method used to investigate the interactions between proteins in biological systems. Proteins are fundamental to life, as nearly all biological processes rely on their functions. These protein-protein interactions are crucial for the execution of these functions. Therefore, understanding the protein interaction network provides insights into the functional mechanisms of organisms. The primary role of protein interaction network analysis is to reveal the complex......

• • Isotop ABPP

  Isotop ABPP is an innovative proteomics technique that integrates the benefits of isotope labeling, tandem mass spectrometry analysis, and protein digestion reactions to comprehensively study protein functions and activation states. In this technique, “isotop” refers to stable isotope tags used to label proteins or their fragments, providing accurate quantification in mass spectrometry analyses. The term “tandem” refers to the multi-stage process in mass spectrometry, where ions are generated through ......

• • Single Cell T Cell Receptor Sequencing

  Single cell T cell receptor sequencing is an advanced technique used to investigate the diversity and specificity of T cells in immune responses. The T cell receptor (TCR) is a protein complex on the surface of T cells that recognizes and binds specific antigen peptides in conjunction with major histocompatibility complex (MHC) molecules, triggering T cell immune responses. The high diversity of TCRs enables them to recognize a broad array of antigens, which underpins the adaptive and specific nature ......

• • Protein Interaction Analysis Overview

  Protein Interaction Analysis aims to uncover the physical and functional relationships between proteins. Proteins, as fundamental molecules of life, form complex networks through interactions, performing various biological functions. Protein interaction analysis has broad applications across multiple fields. In drug development, understanding the interactions of specific proteins aids in identifying drug targets, thus enhancing the selectivity and efficacy of drugs. In disease research, abnormal prote......

• • Edman Degradation Steps

  Edman degradation steps are precise and efficient, primarily comprising: 1. Sample Preparation, ensuring the purity and integrity of the protein sample; 2. Chemical Modification, reacting phenylisothiocyanate (PITC) with the N-terminus of the polypeptide chain to form derivatives; 3. Selective Degradation, cleaving and releasing the labeled N-terminal amino acid; 4. Detection and Identification, identifying the released amino acids through chromatography or mass spectrometry. This series of operations......

• • Determination of Crude Protein Content

  The determination of crude protein content involves quantitatively analyzing the total protein content in a sample using scientific analytical methods. This process is widely applied in fields such as food science, agriculture, animal husbandry, and biotechnology. Crude protein refers to the total protein in a sample and serves as the primary source of nitrogen in both plant and animal tissues, typically estimated through nitrogen content measurement. In the food industry, determining the crude protei......

• • Proximity Extension Assay (PEA)

  Proximity Extension Assay (PEA) is an emerging molecular technique used to detect and quantitatively analyze low-abundance proteins in biological samples. PEA relies on the high specificity of antibodies to target proteins. By attaching unique DNA sequences to two antibodies, these sequences can undergo a proximity extension reaction when the target protein is present, generating a detectable signal. The core principle of PEA is the use of a pair of specific antibodies that bind to distinct epitopes o......

• • Proximity Extension Assay

  Proximity Extension Assay (PEA) is a novel protein detection technique that offers high sensitivity and specificity. The principle behind PEA relies on the binding of two antibodies to the same target protein, with DNA sequences attached to these antibodies becoming sufficiently close to each other. This proximity enables the formation of a detectable double-stranded DNA signal through an extension reaction. By combining the specificity of antibodies with the amplification power of nucleic acids, PEA ......

• • Olink Proteomics in Drug Development and Disease Research

  Olink proteomics leverages innovative technologies developed by Olink to enable precise and high-throughput protein analysis. Central to this platform is the Proximity Extension Assay (PEA) technology, which uses paired antibody-conjugated DNA probes. Upon binding to target proteins, these probes initiate a chain reaction amplification, allowing for the sensitive and specific detection of up to thousands of proteins. This approach has become indispensable in biomedical research, facilitating biomarker......