Resources

Proteomics Databases



Metabolomics Databases

• • Bottom-Up and Top-Down Proteomics

  Bottom-up and top-down proteomics represent two key methodologies in proteomics research, aimed at decoding protein structure, function, and interactions. In bottom-up proteomics, proteins are enzymatically digested into smaller peptides. These peptides are then analyzed using sensitive techniques like mass spectrometry to identify and quantify them. The essence of this method lies in deducing the properties and functions of entire proteins from peptide data. Consequently, bottom-up proteomics is exte......

• • Qualitative Analysis of Amino Acid

  Qualitative analysis of amino acid involves identifying different types of amino acids within biological samples through advanced separation and detection methods. These analyses help elucidate the compositional characteristics and biological functions of amino acids, which are the fundamental units of proteins involved in constructing cellular structures, regulating metabolic pathways, and facilitating neural conduction and signal transmission. Variations in amino acid types and their relative concen......

• • Mass Spectrometry Phosphorylation Site Analysis

  Mass spectrometry phosphorylation site analysis is a powerful technique based on mass spectrometry for identifying and examining phosphorylation sites in proteins. As the most prevalent post-translational modification (PTM), phosphorylation regulates protein activity, stability, and molecular interactions. This modification allows cells to swiftly respond to external stimuli and modulate signaling pathways. Through precise identification of phosphorylation sites, this technique provides critical insig......

• • High-Throughput Protein Sequencing

  High-throughput protein sequencing is a method that utilizes advanced mass spectrometry to rapidly analyze and identify large numbers of proteins. This technique surpasses traditional protein sequencing methods by enabling the analysis of larger sample sizes within shorter time frames, thereby achieving significant advancements in both "quality" and "quantity." The essence of this technology involves the precise mass measurement of peptides by mass spectrometers, coupled with bioinformatics algorithms......

• • Amino Acid LC-MS

  Amino acid LC-MS analysis is a powerful analytical technique that integrates liquid chromatography (LC) and mass spectrometry (MS) to detect and quantify amino acids in complex biological matrices. As fundamental constituents of proteins, amino acids play crucial roles in diverse biological processes such as cellular growth, repair, signaling, and metabolic regulation. Thus, precise measurement of amino acid composition and concentration provides significant insights into biochemical processes, diseas......

• • Glycomics and Glycoproteomics

  Glycomics and glycoproteomics are scientific disciplines focused on the study of carbohydrates within biological systems and their functional roles in cellular processes. Glycomics investigates the structures and functions of carbohydrates, emphasizing their involvement in cell communication and signal transduction pathways. In contrast, glycoproteomics delves into the glycosylation modifications on proteins, analyzing the specific sites and types of modifications and their regulatory effects on prote......

• • Amino Acid HPLC Analysis

  Amino acid HPLC analysis is a critical technique for detecting and analyzing amino acid composition and content in samples. This method, widely employed in analytical chemistry, achieves compound separation through the passage of a liquid mobile phase over a solid stationary phase. Amino acids, as the fundamental building blocks of proteins in organisms, are crucial in various fields such as biomedicine, nutrition, food science, and drug development. With its high resolution, sensitivity, and reproduc......

• • Protein Extraction and Quantification

  Protein extraction and quantification are essential procedures in biological science research, with widespread applications in fields such as biomedicine, drug discovery, and agricultural science. Proteins are fundamental to life, playing key roles in cell structure, metabolic regulation, and signal transduction. To gain insights into these functions, scientists extract and quantify proteins from cells or tissues. The primary goal of protein extraction is to isolate proteins from other cellular compon......

• • HPLC Protein Quantification

  HPLC protein quantification is a precise analytical method used to determine the concentration of proteins in samples utilizing the high resolution and sensitivity of HPLC systems. This technique is widely applicable across various fields, including biopharmaceuticals, food science, and environmental science. In biopharmaceuticals, it assists in drug development and quality control by ensuring protein content in pharmaceuticals complies with standards. In food science, it facilitates the detection of ......

• • C-Terminal Sequencing: 8 Critical Mistakes to Avoid in LC-MS/MS Analysis

  LC-MS/MS analysis is extensively applied in proteomics for protein identification, post-translational modification profiling, and quantitative studies. However, during C-terminal sequencing, researchers often make avoidable mistakes in experimental design, data acquisition, and data analysis, which can compromise data quality and the reliability of conclusions. This article highlights eight critical mistakes commonly encountered in LC-MS/MS-based C-terminal sequencing, and provides practical strategie......