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Proteomics Databases



Metabolomics Databases

• • High-Throughput Immunopeptidomics Profiling For Drug Target Identification

  Target discovery remains a central challenge in modern drug development. This is particularly evident in complex pathological contexts, including cancer, infectious diseases, and autoimmune disorders, where the accurate identification and validation of druggable targets critically determine the success of therapeutic development. In recent years, immunopeptidomics, a mass spectrometry-driven analytical approach, has emerged as a powerful strategy for drug target identification and validation. What Is......

• • End-to-End Immunopeptidomics Services: From Sample to Neoantigen Discovery

  Tumor immunotherapy has undergone rapid advancement in recent years. From immune checkpoint inhibitors to personalized vaccines and TCR-T cell therapies, strategies aimed at precisely activating T cell recognition of tumor cells are increasingly regarded as a central direction in next-generation cancer treatment. Neoantigens, defined as novel peptide sequences arising from tumor-specific genetic mutations, represent ideal targets for eliciting tumor-specific immune responses due to their strong non-se......

• • How Does ABPP Support High-Throughput Compound Screening?

  In the early stages of drug discovery, researchers often need to screen hundreds or even thousands of candidate compounds to identify molecules with favorable potency, selectivity, and safety profiles. Traditional screening approaches, such as enzyme inhibition assays and binding affinity analyses, are amenable to high-throughput implementation, but they generally lack direct functional readouts at the target level. As a result, they cannot provide clear evidence that a compound is exerting its intend......

• • Step-by-Step Protocol for Conducting ABPP Experiments

  Unlike conventional proteomics approaches, ABPP (Activity-Based Protein Profiling) not only identifies protein expression, but also captures protein activity states. It is particularly well suited for functionally important enzyme classes with complex regulatory behavior, such as proteases, phosphatases, and deacylases. How, then, can a complete ABPP experiment be systematically designed and implemented? From probe selection to mass spectrometric analysis, and from sample preparation to data interpret......

• • How to Analyze Histone β-Hydroxybutyrylation by LC-MS/MS?

  Liquid chromatography-tandem mass spectrometry (LC-MS/MS) provides a highly sensitive and specific method for analyzing histone β-hydroxybutyrylation (β-hydroxybutyrylation, Kbhb), enabling the elucidation of key roles of epigenetic modifications in metabolic regulation and disease. Histone β-hydroxybutyrylation is a recently identified lysine post-translational modification, with its acyl group derived from the ketone body β-hydroxybutyrate (BHB). This modification is closely linked to energy metabol......

• • What Is Histone β-Hydroxybutyrylation (Kbhb)?

  Histone β-hydroxybutyrylation (Kbhb, lysine β-hydroxybutyrylation) is a novel post-translational modification (PTM) characterized by the covalent attachment of a β-hydroxybutyryl group to lysine residues (Lys), representing a type of short-chain fatty acid acylation. The discovery of Kbhb reveals a direct link between cellular metabolites and epigenetic regulation, emerging as a research focus at the intersection of epigenetics and metabolomics in recent years. Chemical Nature and Formation Mechanism......

• • Applications of Bottom-Up Proteomics in Clinical Research

  Proteins directly reflect cellular states, disease progression, and therapeutic responses. With the rapid advancement of mass spectrometry technologies, Bottom-Up proteomics has become a key methodological approach in clinical research for investigating disease mechanisms, discovering biomarkers, and optimizing personalized treatment strategies. This article provides an in-depth analysis of the major applications of Bottom-Up proteomics in clinical research and introduces how MtoZ Biolabs delivers pre......

• • Top 6 Tools to Analyze Bottom-Up Proteomics Data More Efficiently

  In Bottom-Up proteomics (also referred to as shotgun proteomics), protein samples are enzymatically digested and analyzed in the form of peptides, from which the corresponding proteins are subsequently inferred. This workflow involves multiple steps, including mass spectrometry data processing, peptide identification, protein inference, and quantitative analysis. In Bottom-Up proteomics, the efficiency and accuracy of data analysis directly influence the depth and reliability of research outcomes. The......

• • Which Sample Preparation Methods Improve Histone Phosphorylation Detection?

  In epigenetics and cell signaling research, histone phosphorylation remains challenging to analyze because of its low abundance, highly dynamic nature, and site diversity. Compared with conventional proteomics workflows, the detection of histone phosphorylation is more strongly dependent on sample preparation quality. A poorly designed sample preparation workflow often results in signal loss, limited site coverage, or poor data reproducibility. Which sample preparation methods, then, can significantly......

• • How Are Histone Phosphorylation Sites Identified?

  Histones constitute critical components of chromatin, and their post-translational modifications (PTMs) are essential for regulating gene expression, chromatin remodeling, and the cell cycle. Among these modifications, phosphorylation is a dynamic and rapidly responsive PTM that is crucial for biological processes such as DNA damage repair and mitosis. Accurate identification of histone phosphorylation sites is a foundational step for systematically investigating their biological significance. Challe......