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    • • Peptide Sequencing Techniques: Challenges and Breakthroughs

      What Is Peptide Sequencing? Why is it so Critical? Peptides are short chains composed of 2–50 amino acid residues, which can be degradation products of proteins, functional signaling molecules, bioactive drugs, or even vaccine antigen epitopes or TCR peptide segments. The primary objectives of peptide sequencing include: Confirming the amino acid sequence of antibody CDR regions. Identifying endogenous bioactive peptides (such as hormones and cytokines). Verifying the structure of degradation products......

    • • How to Perform High-Precision Antibody Sequencing?

      Antibody sequencing has garnered significant attention in recent years as a crucial tool for the development of antibody drugs and the characterization of antibodies. In particular, scenarios such as CDMO contract manufacturing, antibody humanization, patent portfolio design, and consistency analysis have imposed increasingly stringent requirements on the accuracy, completeness, and functional site identification of antibody sequences. How can high-precision antibody sequencing be achieved? What key steps..

    • • Protein Mass Spectrometry Analysis: How to Improve Accuracy and Sensitivity

      Protein mass spectrometry (MS) analysis, as a core technology in modern proteomics, has been widely employed in studies of disease mechanisms, drug target identification, and biomarker discovery. However, enhancing the accuracy and sensitivity of protein mass spectrometry analysis remains a critical challenge in practical applications. Sample Preparation: The Foundation for High-Quality Data The quality of sample preparation plays a decisive role in determining the performance of subsequent mass .......

    • • Comprehensive Analysis of Protein Sequence: Methods and Challenges

      The amino acid sequence of a protein fundamentally underlies its structure and function. Comprehensive protein sequence analysis not only reveals the molecular identity of a protein but also provides critical insights into its functional mechanisms, disease-associated variations, and target drug design. In the current era of rapid advancement in omics research, achieving efficient and accurate determination of protein primary structures has become an indispensable and pivotal technical aspect of .......

    • • History and Trends in Protein Sequencing

      The history of Protein Sequencing technology has marked the transition of modern life sciences from chemical reactions to system-level omics. From the pioneering Edman degradation method to today’s proteomics platforms centered around high-resolution mass spectrometry, the technological evolution of Protein Sequencing has continually expanded the depth, throughput, and functional analytical capacity of detection, laying a robust foundation for decoding the blueprint of life. This paper reviews the pivotal..

    • • Overview of Protein Sequencing: Advantages and Limitations

      Proteins act as the primary executors of biological functions, with their structure and functionality intricately dependent on the precise arrangement of amino acid sequences. Protein sequencing technology serves as a crucial bridge linking genomics and phenotypic research, advancing our comprehension of biological systems. Utilizing high-resolution mass spectrometry, protein sequencing enables comprehensive qualitative and quantitative characterization of proteins by analyzing peptide fragmentation .......

    • • Recommendations for 5 Commonly Used Protein Sequence Alignment Tools

      Introduction: Why Is Protein Sequence Alignment so Important? The function of a protein is often reflected in its amino acid sequence. Conserved regions, active sites, and modification sites within the sequence serve as critical indicators for predicting protein function. By aligning a target sequence with known protein sequences in public databases, researchers can: annotate the functions of uncharacterized proteins; identify homologous proteins and evolutionary relationships; detect domains, binding......

    • • How to Choose the Right Protein Sequencing Technology?

      Protein sequencing is a fundamental technique for investigating protein structure and function. It plays a critical role in biomedical research and drug development, including target discovery, mechanism elucidation, biomarker identification, and antibody characterization. With continuous advancements in mass spectrometry and chemical sequencing methods, an increasing number of protein sequencing technologies have become available. These technologies differ significantly in their suitable applications......

    • • Latest Applications of AI in Protein Sequencing

      In the current era of AI-driven transformation in the life sciences, artificial intelligence (AI) is redefining the technological framework of protein sequencing. From structural prediction to de novo sequencing, from identification of post-translational modification (PTM) sites to sequence reconstruction, AI technologies are being increasingly integrated into all facets of protein analysis. These advancements not only enhance efficiency and reduce error rates but also empower researchers to better manage..

    • • What Tools Are Used for Protein Sequence Analysis? A Summary of Common Methods and Techniques

      In fields such as proteomics, structural biology, functional annotation, and drug discovery, protein sequence analysis frequently serves as a foundational step in research. This type of analysis not only determines the primary structure of proteins but also supports the prediction of molecular features such as functional domains, signal peptides, transmembrane regions, post-translational modification sites, and linear antigenic epitopes. It constitutes a critical component in identifying potential targets..

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