Resources
Proteomics Databases
Metabolomics Databases
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Label-Free Quantification (LFQ) is a form of quantitative proteomics that eliminates the need for isotopic or fluorescent labeling of samples. By assessing the relative abundance of peptides in a sample through mass spectrometry, LFQ delivers high sensitivity and throughput in protein quantification while preserving the sample's structural integrity. LFQ crucially examines variations in relative protein abundance across different samples or treatment conditions, thereby unveiling potential biological ......
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Tandem Mass Tag (TMT) is a widely-used chemical labeling method in proteomics research, which leverages isotopic tags of varying masses to label proteins across different samples. These tags consist of three components: a reporter group, a balance group, and a reactive group. The reactive group specifically binds to amino groups on proteins, covalently attaching the tag. In tandem mass spectrometry, peptides labeled with Tandem Mass Tag (TMT) from different samples show identical mass in the primary s......
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Multidimensional Protein Identification Technology (MUDPIT) is a high-throughput proteomics method that integrates liquid chromatography (LC) and mass spectrometry (MS). It is particularly effective for identifying and quantifying complex protein mixtures, such as those found in cell lysates, tissue samples, and subcellular components. By enhancing the depth and coverage of protein identification, MUDPIT is widely utilized in biomedical research, drug development, and studies of disease mechanisms. De......
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Bottom-up mass spectrometry is a classical analytical technique in proteomics used for detailed analysis of complex protein samples' amino acid sequences. Unlike traditional top-down methods, this approach involves enzymatically digesting intact proteins into smaller peptide fragments, which are then analyzed using mass spectrometry to obtain structural information. This method is particularly effective for large-scale protein identification and quantification and is widely applied in fields such as p......
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Polysaccharide analysis serves as a critical methodology for elucidating the molecular structure, function, and interactions of complex carbohydrate polymers. Polysaccharides, consisting of monosaccharide units linked by glycosidic bonds, are prevalent in plants, animals, and microorganisms. These macromolecules fulfill essential physiological roles, including energy storage, structural support, cellular recognition, and signal transduction. The principal objective of polysaccharide analysis is to det......
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• Interaction Network Analysis
Interaction network analysis is a prominent technique in biological research that seeks to elucidate the complex biological processes within cells and organisms by examining molecular interactions. In the cellular environment, molecules such as proteins, nucleic acids, and lipids interact intricately to sustain essential life functions. By constructing networks of these interactions, interaction network analysis aids researchers in deciphering cellular mechanisms, understanding disease pathogenesis, a......
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• DNA and Protein Sequence Analysis
DNA and protein sequence analysis are crucial techniques in biological research, involving sequencing, alignment, and interpretation of genomic DNA and the protein sequences encoded by genes. This analysis reveals the genetic information and biological functions of organisms. DNA sequence analysis primarily interprets genetic information within the genome, such as gene mutations and gene expression. In contrast, protein sequence analysis focuses on the amino acid composition, structure, function, and ......
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Discover protein involves identifying, characterizing, and analyzing novel or unknown proteins in biological samples using experimental techniques and computational approaches. Proteins are critical to life, serving essential roles in cellular metabolism, signal transduction, immune response, and disease development. Thus, discover protein is vital not only for basic biological research but also for the identification of disease biomarkers, the discovery of new drug targets, and the advancement of per......
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Protein profile analysis is an indispensable core technology in modern biological research. It employs a mass spectrometer to measure the mass-to-charge ratio (m/z) of proteins, thereby accurately analyzing their composition, structure, post-translational modifications, and interactions. As key molecules within cells, proteins are responsible for nearly all biological processes-including catalysis, structural support, and signal transduction-so protein profile analysis provides critical insights into ......
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protein expression profile is a pivotal technique for studying the types, quantities, and dynamics of proteins within an organism. By systematically analyzing proteins, this technique elucidates protein expression patterns under various physiological or pathological conditions. As the most diverse and functionally versatile molecules in cells, proteins play crucial roles in biological processes such as cell growth, differentiation, signal transduction, and immune response. Thus, understanding protein ......
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