Resources

Proteomics Databases



Metabolomics Databases

• • Principle of Mass Spectrometry for Detecting Ubiquitination

  The ubiquitin-proteasome pathway constitutes a major mechanism of protein degradation and plays a critical role in maintaining intracellular protein homeostasis. Ubiquitination, a post-translational modification, involves the covalent attachment of the small protein ubiquitin to target substrates. Mass spectrometry has emerged as a powerful and indispensable tool for investigating ubiquitination. In the following sections, we provide a detailed examination of the fundamental principles underlying the.......

• • How to Perform Protein Sequence Analysis?

  Protein sequence analysis involves examining the amino acid sequences of proteins to elucidate their structures and functions. This analytical approach transcends basic structural characterization, providing critical insights into protein functionality, molecular interactions, disease mechanisms, and therapeutic targets. Proteins exhibit remarkable diversity, with each protein's unique three-dimensional structure directly linked to its specific function, determined by its amino acid sequence. Understa......

• • Global Histone Post-Translational Modifications

  Histone post-translational modifications (PTMs) play a crucial role in the regulation of gene expression. By adding or removing chemical groups such as acetyl, phosphate, and methyl, these modifications can alter the charge distribution and conformation of histones. This in turn affects chromatin compaction and gene accessibility, facilitating precise control over gene expression.

• • Sugar Alcohol LC-MS Detection

  Sugar alcohols, also known as polyols, are a class of biologically significant natural products with diverse functions. They participate in numerous biochemical reactions within organisms and contribute to various physiological processes, including signal transduction, stabilization of cell membranes, antioxidative mechanisms, and stress responses. Consequently, sugar alcohols and their metabolites hold considerable importance in biochemical research, cell biology, and medical studies. Liquid chromatography

• • Total Redox-Modified Proteome

  Redox modification is an important form of protein modification within organisms, regulating the redox state of proteins and thereby controlling their structure, function, and activity. In general, redox modifications can be divided into oxidation modifications and reduction modifications.

• • Analysing Disulfide Bonds Within Proteins

  Disulfide bonds are a key contributor to protein stability, essential for maintaining the three-dimensional structure and biological functionality of proteins. Therefore, investigating the disulfide bonds within proteins can enhance our understanding of protein structure and function. In biological contexts, disulfide bonds are covalent linkages formed between sulfur atoms within proteins, specifically between cysteine residues. These bonds are crucial for various biological processes, including protein....

• • Protein Interaction Validation

  Protein interaction verification is an essential aspect of biological research, designed to determine whether proteins engage in direct or indirect interactions with one another. This process is instrumental in elucidating intracellular signaling pathways, protein functions, and the pathogenesis of diseases.

• • Mutual Protein Identification

  Identification of interacting proteins refers to the process of recognizing and analyzing interactions between proteins, which is crucial for understanding cellular functions and disease mechanisms. There are various methods for identifying interacting proteins, each with its own advantages and limitations. Here we present a detailed overview of several commonly used methods:

• • Plant Mass Spectrometry Detects Protein Interactions

  Using mass spectrometry to study protein-protein interactions in plants represents a significant direction in contemporary plant proteomics research. By analyzing protein interaction networks, scientists gain deeper insights into physiological processes such as plant growth and development, environmental adaptation, and disease defense. This approach not only identifies direct interactions between specific proteins but also elucidates the composition of protein complexes and their physiological roles in....

• • Mass Spectrometry Combined with Protein

  Mass spectrometry combined with proteomics represents a powerful analytical tool for the identification and quantification of proteins within cells. This process can generally be divided into the following steps: