Resources
Proteomics Databases
Metabolomics Databases
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Direct protein sequencing is a technique used to determine the primary structure of proteins by analyzing their amino acid sequences. Unlike genome- or transcriptome-based approaches, it directly characterizes protein sequences without relying on genetic information. Traditional protein sequencing methods have primarily relied on DNA or mRNA sequencing to infer protein sequences. However, due to post-transcriptional modifications, alternative splicing, and non-canonical translation, genomic data do no......
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The cancer methylome refers to the genome-wide DNA methylation landscape in cancer cells, encompassing methylation status changes that influence gene expression and contribute to tumorigenesis. DNA methylation is an essential epigenetic regulatory mechanism that involves the addition of a methyl (-CH3) group to the 5th carbon of cytosine (C) residues in DNA, forming 5-methylcytosine (5mC). This modification regulates gene transcription without altering the underlying DNA sequence. In normal cells, DNA......
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Peptide array analysis is a high-throughput and high-precision technology extensively utilized in proteomics and molecular biology research. This technique involves immobilizing a diverse array of peptide sequences onto solid-phase supports (such as glass slides or microarray chips) to construct a peptide array, which is subsequently employed to investigate the binding properties of these peptides to target molecules, including antibodies, proteins, and enzymes. This approach enables the efficient and......
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BioID&APEX (Atypical Protein Labeling by Tethered Enzymes) are two innovative methodologies that have gained traction for mapping the intricate networks of protein interactions within cells. These technologies leverage enzyme-mediated labeling to capture proteins located in proximity to a target protein, offering an efficient alternative to traditional methods like immunoprecipitation. Unlike these techniques, which require direct contact between interacting proteins, BioID&APEX utilize the catalytic ......
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Methylome profiling is a technique used to investigate DNA methylation modifications, providing insights into methylation level variations and facilitating the understanding of complex biological processes such as gene expression regulation, cell differentiation, and disease pathogenesis. DNA methylation involves the addition of a methyl (-CH3) group to the 5th carbon position of cytosine (C) bases within DNA molecules, a modification that plays a crucial role in regulating gene expression activity. ......
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Zebrafish proteomics systematically investigates the composition, functions, and roles of all proteins within zebrafish using high-throughput mass spectrometry techniques. As a widely utilized model organism, zebrafish are advantageous for studies in developmental biology, disease mechanisms, and drug screening due to their rapid growth, defined genetic background, and transparent embryos. Through zebrafish proteomics research, scientists gain valuable insights into developmental processes, immune res......
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• Protein Mass Spectrometry Data Analysis
Protein mass spectrometry data analysis is crucial in contemporary proteomics research, enabling the precise identification and quantification of proteins within complex biological samples via mass spectrometry techniques. As a formidable tool, mass spectrometry unveils insights into protein structures, abundances, modifications, and interactions. Nevertheless, the vast and intricate datasets generated necessitate the extraction of meaningful information and the execution of precise analyses and inter......
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timsTOF proteomics represents a cutting-edge approach in proteomics analysis, leveraging a combination of Time-of-Flight (TOF) mass spectrometry and Trapped Ion Mobility Spectrometry (TIMS). The primary advantage of this technology lies in its ability to deliver high-resolution and high-sensitivity analyses of proteins, facilitating the precise identification and quantification of proteins within complex biological matrices. Unlike traditional mass spectrometry techniques, timsTOF offers a unique capa......
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• Total Protein Quantification
Total protein quantification involves measuring the overall protein concentration in biological samples like cells, tissues, blood, or other bodily fluids. This process is essential for providing a reliable foundation for subsequent biochemical analyses, proteomics investigations, and biomarker screenings. Accurate quantification is critical in protein-related experiments to ensure data comparability and result reliability. Whether involved in protein extraction, electrophoresis, immunoblotting (Weste......
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• Subcellular Proteomics: Insights into Cellular Function
Subcellular proteomics technology is an approach designed to elucidate the molecular mechanisms within distinct cellular compartments by analyzing the expression, functionality, and interactions of proteins. Unlike conventional holistic cellular proteomics, subcellular proteomics focuses on the dynamic distribution and interaction of proteins within spatial regions of the cell, thereby revealing their precise roles in cellular physiology and pathology. By conducting quantitative and qualitative assess......
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