Resources

Proteomics Databases



Metabolomics Databases

• • Protein Amino Acid Sequence Determination

  The determination of the amino acid sequence of a protein is referred to as protein sequence determination. Here is a basic introduction to amino acid sequence determination.

• • Analysis of Protein Sequencing Data Types

  Protein sequencing typically refers to determining the amino acid sequence of a protein. This involves sorting the amino acids of a protein to determine their exact order within the protein.

• • Application of Label Transfer in Protein Interaction Studies

  The core mechanism of the label transfer technique involves using specific labeling reagents to link a target protein with interacting proteins, thereby enabling precise protein interaction analysis. 

• • Workflow of Label Transfer Protein Interaction Analysis

  The core mechanism of the label transfer method involves treating bait proteins with labeling reagents, followed by crosslinking these reagents to prey proteins through light exposure or other activation methods. The detailed steps are as follows:

• • Advantages and Disadvantages of Label Transfer in Protein Interaction

  In protein interaction studies, the label transfer method, combined with crosslinking techniques, provides an effective means of marking proteins that interact with target proteins. This technique not only facilitates the discovery of new interactions but also validates proteins identified by other methods and analyzes protein complexes.

• • Principle of Label Transfer in Protein Interaction Analysis

  The core of the label transfer method involves transferring a label from one protein (typically referred to as the "bait") to another interacting protein (referred to as the "prey"). This method leverages the photosensitive properties of crosslinkers, which form covalent bonds between two interacting proteins when exposed to light at a specific wavelength.

• • Mechanism of SILAC and Dimethyl Labeling in Quantitative Proteomics

  The advancement of quantitative proteomics has significantly enhanced our understanding of protein expression, modification, and interaction within biological systems. Among various quantitative proteomic techniques, SILAC (Stable Isotope Labeling by Amino acids in Cell culture) and dimethyl labeling have become widely adopted due to their high sensitivity and quantitative accuracy.

• • Application of SILAC and Dimethyl Labeling in Quantitative Proteomics

  Stable Isotope Labeling by Amino acids in Cell culture (SILAC) and Dimethyl Labeling are two pivotal techniques in quantitative proteomics. As proteomics research progresses, scientists increasingly depend on these techniques for the precise quantification of protein expression changes.

• • Workflow of SILAC and Dimethyl Labeling in Quantitative Proteomics

  Quantitative proteomics often relies on methods such as SILAC (Stable Isotope Labeling by Amino acids in Cell culture) and dimethyl labeling for comparing the relative abundance of proteins across different samples. Each method has distinct advantages, making them suitable for various experimental conditions.

• • Advantages and Disadvantages of SILAC and Dimethyl Labeling in Quantitative Proteomics

  Quantitative proteomics is a critical technology for studying changes in protein expression under various biological conditions. Among the numerous quantitative proteomics techniques, SILAC (Stable Isotope Labeling by Amino acids in Cell culture) and dimethyl labeling are two widely adopted methods.