Resources
Proteomics Databases
Metabolomics Databases
-
Subtractive proteomics is a methodological approach designed to enhance the detection and analysis of target proteins within complex biological samples by eliminating or reducing the presence of interfering components. Unlike traditional proteomics that provide a comprehensive overview of the protein landscape, subtractive proteomics employs a strategic "subtraction" method to remove irrelevant proteins or background noise. This refined focus enables researchers to delve deeper into the functional rol......
-
Interaction proteomics is a powerful approach for systematically characterizing protein interaction networks, providing insights into their functional roles and regulatory mechanisms in biological systems. Unlike traditional proteomics, which focuses on individual proteins, interaction proteomics integrates multiple analytical strategies to examine protein-protein, protein-DNA, and protein-RNA interactions, elucidating their contributions to cellular processes. Applications in Biomedical Research Pr......
-
• Structural and Functional Proteomics
Structural and functional proteomics is a scientific discipline focused on elucidating the relationship between protein structure and function. By integrating the strengths of structural biology and functional proteomics, it aims to uncover the specific mechanisms by which proteins operate both within and outside the cell. As proteins are among the most complex and diverse biomolecules essential to nearly all biological processes, understanding their structure and function is crucial for addressing nu......
-
Integrated proteomics is an interdisciplinary approach that employs multiple analytical techniques to systematically investigate the entire proteome of an organism. Unlike conventional proteomics, which primarily focuses on qualitative and quantitative protein profiling, integrated proteomics incorporates diverse experimental strategies and advanced computational analysis to explore post-translational modifications (PTMs), protein-protein interactions, and the functional roles of proteins in complex b......
-
ICAT proteomics (Isotope-Coded Affinity Tag proteomics) is a mass spectrometry-based technique that enables accurate and high-throughput quantitative proteomic analysis. Its fundamental principle involves labeling cysteine-containing proteins with isotope-coded affinity tags, allowing for the comparative quantification of proteins across different biological samples. This method is particularly advantageous in complex proteomic studies, facilitating the identification and quantification of differentia......
-
• Mass Spectrometry Biomarker Discovery
Mass spectrometry biomarker discovery leverages mass spectrometry techniques to identify and quantify specific molecular biomarkers within complex biological samples. Biomarkers, which can include proteins, metabolites, or other biomolecules, serve as indicators of physiological or pathological states. Cancer research is a prominent application area of this technology. By comparing biological samples from healthy individuals with those from cancer patients, researchers can pinpoint potential cancer-sp......
-
• Thermal Shift Assay Drug Discovery
The thermal shift assay (TSA) is a widely used biophysical method for assessing protein thermal stability and has become an essential tool in thermal shift assay drug discovery. Its fundamental principle is based on the observation that small-molecule binding can alter a protein’s conformational stability, leading to measurable shifts in its melting temperature (Tm). By tracking the protein melting curve with a fluorescent probe, researchers can quantify ligand binding strength and obtain critical dat......
-
• Densitometry Protein Quantification
Densitometry protein quantification is a quantitative analytical method that relies on the principle of substance density changes. It harnesses the relationship between the density of a protein solution and its concentration to accurately determine protein content. In proteomics research, precise protein quantification is essential for deciphering biological mechanisms, discovering disease biomarkers, and drug development. Traditional protein quantification methods, such as ultraviolet absorption (UV)......
-
MALDI proteomics is an advanced mass spectrometry technique used for the efficient analysis of proteins and large biomolecules. When compared to traditional mass spectrometry methods, MALDI proteomics offers enhanced detection sensitivity, higher throughput, and requires less sample material. This technology enables precise analysis of complex biological samples, allowing researchers to identify protein mass, structure, and interactions. MALDI proteomics has been applied across various fields. In prot......
-
• Thermal Shift Stability Assay
The thermal shift stability assay is a widely used method for assessing protein stability across different temperature conditions. Protein stability refers to the ability of a protein to retain its native conformation under specific environmental parameters. This assay evaluates the thermal stability of proteins by tracking temperature-dependent conformational transitions, providing valuable insights into protein folding and stability. A key parameter in this analysis is the melting temperature (Tm), ......
How to order?
