Resources

Proteomics Databases



Metabolomics Databases

• • Principle of SILAC and Dimethyl Labeling in Quantitative Proteomics

  Proteomics is the study of the composition, structure, function, and dynamic changes of proteins within cells or tissues. In quantitative proteomics, researchers employ various labeling techniques to measure the relative or absolute abundance of proteins, with stable isotope labeling being highly regarded for its precision and reliability.

• • How to Calculate the Isoelectric Point of Amino Acids?

  The isoelectric point (pI) of an amino acid refers to the pH value at which the net charge of the amino acid molecule is zero. The basic method for calculating the pI of an amino acid involves considering the pKa values (negative logarithm of acid dissociation constants) of the functional groups in the amino acid molecule, and using these values to find the pH value at which the net charge of the amino acid molecule is zero.

• • Chiral Circular Dichroism of Amino Acids

  Due to the presence of a chiral center on the α-carbon, amino acids exhibit optical activity. The two stereoisomers of chiral amino acids are referred to as L and D forms. The majority of naturally occurring amino acids in proteins are of the L form. As these amino acids are optically active, they interact with plane-polarized light, resulting in the rotation of the light.

• • Peptide Drug Elemental Analysis

  Peptide drugs are biologically active molecules composed of multiple amino acids linked by peptide bonds. Typically, they consist of 10-100 amino acids and have a molecular weight below 10,000. Peptide drugs are mostly derived from endogenous or natural peptides, therefore they have little or no side effects on the human body.

• • Recombinant Protein Drug FT-IR Analysis

  Recombinant protein drugs are protein products derived from animals and plants through biotechnological research and development, with certain biological activities that can prevent, treat, and diagnose diseases in humans, animals, and plants. Compared to small molecule drugs, recombinant protein drugs have advantages such as high activity, high specificity, and low toxicity, making them favored by researchers.

• • Protein Sequencing: Key Technology for Decoding the Code of Life

  Protein sequencing is the process of determining the specific arrangement of amino acids in a protein. This sequence information not only provides identity markers for proteins but also reveals their structural and functional relationships. For example, proteins within the same family often exhibit sequence similarity, implying similar functions or origins.

• • Protein Primary Structure Analysis: Importance of Amino Acid Sequence

  The primary structure of a protein is its most basic level of structure, referring to the linear sequence of amino acids in the protein. The amino acid sequence of a protein determines the way it forms higher-level structures and its ultimate function. Therefore, analyzing the primary structure of a protein is a crucial step in biochemical and molecular biology research.

• • De Novo Sequencing for Protein Structure and Function Analysis

  "De novo" (from scratch) sequencing is a technique used to determine the amino acid sequence of new or unknown proteins or peptides. This method is particularly useful for proteins or peptides that are not present in existing databases.

• • Analysis of Impurities in Recombinant Protein Drug Products

  Recombinant protein drugs are biopharmaceutical products produced by engineered cells, with complex molecular structures and relatively complex manufacturing processes. During the production process, not only process-related impurities such as host cell proteins and DNA residues may be generated, but also product-related impurities may be produced. These product-related impurities mainly include degradation products, aggregates, and variants.

• • Determination of Antibody Drug Protein Concentration

  Antibody drugs are a class of drugs that are artificially synthesized antibodies used to treat diseases. They achieve therapeutic effects by specifically binding to target molecules. Common types of antibody drugs include monoclonal antibodies, artificially synthesized antibody fragments, immunotoxins, and antibody-drug conjugates.