Resources
Proteomics Databases
Metabolomics Databases
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The in vitro pull-down assay, also known as the protein pull-down assay, is primarily used to study the interactions between proteins, proteins and nucleic acids, or other biomolecules. In this assay, the protein of interest (referred to as the "bait") is typically immobilized on a solid-phase carrier, such as agarose or magnetic beads, and then a solution containing other proteins that may interact with the bait (referred to as the "prey") is added to the reaction system. Through a series of washes a......
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Ligand-receptor analysis is a crucial technique used to investigate the interactions between biomolecules, particularly the binding between a ligand and its corresponding receptor. Ligands are typically small molecules, peptides, or proteins, while receptors are predominantly transmembrane proteins located on the cell membrane that can detect and transmit external signals. The binding of ligands to receptors forms the foundation of cellular communication, signal transduction, and the regulation of phy......
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• Glycoprotein Analysis Techniques and Applications
Glycoprotein analysis is a specialized technique aimed at studying the structure and function of glycosylated proteins. Its primary goal is to reveal the glycan composition, glycosylation sites, glycan structure, and their relationship with protein function. Glycosylation, a common post-translational modification, is prevalent in the cells of animals, plants, and microorganisms. Glycoprotein analysis encompasses a wide range of studies, including glycan structure analysis, glycosylation site identific......
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• Disulfide Bond Analysis in Protein Research
Disulfide bond analysis is a key method for investigating protein structure and function. Disulfide bonds are covalent bonds formed between two cysteine residues, and they play a crucial role in maintaining the three-dimensional structure, stability, and biological activity of proteins. By performing disulfide bond analysis, scientists can gain insights into the correct folding of proteins, which is essential for understanding their function and interactions. The applications of disulfide bond analysi......
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• Can Disulfide Bond Identification Reveal Protein Structural Stability?
Disulfide bonds, as a special type of chemical bond, play a key role in the structural stability of proteins. However, whether the disulfide bond identification can accurately reveal the stability of protein structures has been a topic of scientific interest.
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Biotherapeutic analysis refers to a comprehensive technical framework for the structural, functional, and characterization analysis of biotherapeutic-related molecules such as proteins, antibodies, nucleic acids, and cell-based products. Biotherapy, an emerging therapeutic approach that leverages biological molecules and cellular technologies, includes monoclonal antibody drugs, gene therapy, cell therapies, and vaccines. These modalities have demonstrated considerable potential in treating a wide ran......
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• Can Protein Structure Analysis Reveal Key Insights into Protein Function and Interactions?
Protein structure analysis is a key method for studying the three-dimensional conformation and spatial structure of proteins, which are important functional molecules in organisms.
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• What Methods are Used for Protein Differential Expression Analysis?
Protein differential expression analysis is of great significance in biological research, drug development, and personalized medicine, as it helps to reveal biological processes, discover new therapeutic targets, and study individualized medicine. Methods and Steps of Protein Differential Expression Analysis 1. Sample Preparation First, collect samples under different conditions or groups, and carry out protein extraction and purification to obtain high-quality protein samples. 2. Protein Analysis......
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• Principles and Methods of Plasma Analysis in Proteomics
Blood is one of the most important biological fluids in the human body, containing a wealth of protein resources. Proteomics plasma analysis is a powerful technique with the advantage of studying the composition and function of proteins in blood. Through plasma proteomics analysis, we can comprehensively understand the changes of proteins in blood and discover potential biomarkers related to diseases. This provides an important basis for the early diagnosis and treatment strategy formulation of blood-relate
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• Innovative Applications of Protein Mass Spectrometry in Biomedicine and Food Safety
Protein mass spectrometry analysis, as an efficient, sensitive, and accurate technique, has revealed the mysteries of the protein world and demonstrated innovative applications in various fields. Applications of Proteomic Analysis in Biomedical Research 1. The Development of Proteomics Proteomics is the study of all proteins in organisms, including their composition, structure, function, and interactions. Protein mass spectrometry analysis, as the core technology of proteomics, has become an importa......
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