Resources

Proteomics Databases



Metabolomics Databases

• • End-Group Analysis of Protein

  End-group analysis of protein is a technique in proteomics research primarily used to determine the amino acid sequences at both termini of protein molecules. Typically, protein ends include the N-terminus (amino terminus) and the C-terminus (carboxyl terminus). This analysis not only provides structural information but also elucidates various biological functions. For example, the N-terminus is closely linked to signal peptide recognition, protein localization, and cellular half-life, while the C-ter......

• • Detecting Phosphorylated Proteins

  Detecting phosphorylated proteins refers to the process of identifying and analyzing the presence and localization of phosphate groups on protein molecules using specific experimental techniques and methods. Phosphorylation, the most common type of post-translational modification (PTM) in proteins, regulates protein activity, localization, and interactions by adding phosphate groups to serine, threonine, or tyrosine residues. It plays a critical role in biological processes such as cell signaling, met......

• • Capillary Electrophoresis Protein Analysis

  Capillary electrophoresis protein analysis is a highly effective separation technique that leverages narrow-diameter capillaries and high-voltage electric fields to facilitate the movement of protein molecules in an electrolyte solution, leading to efficient separation and analysis. This method is extensively employed in biochemical and molecular biology research for the purposes of protein separation, purification, characterization, and quantitative analysis. The principle underlying this technique d......

• • Characterization of Biologics and Biosimilars

  Characterization of biologics and biosimilars is a critical step in evaluating their quality, safety, and efficacy. Biologics refer to therapeutic proteins, antibodies, vaccines, cell therapy products, and similar agents produced by living cells, and are widely used in the treatment of cancer, autoimmune disorders, and rare diseases. Owing to the complex structure of biologics, their characterization involves a variety of analytical techniques and rigorous quality control methods to ensure drug safety......

• • Residual Protein Analysis

  Residual protein analysis is a technique employed to detect and quantify trace proteins in biological samples and processed products, significantly impacting the biopharmaceutical and food processing sectors. In the biopharmaceutical industry, ensuring that drug product impurities remain within safe limits is crucial for guaranteeing safety and efficacy. Similarly, the food processing industry must prevent foreign proteins introduced during production from posing allergenic or health risks to consumer......

• • Secretome Proteomics

  Secretome proteomics is a branch of modern biotechnology research dedicated to investigating the composition and function of proteins secreted by cells. By examining these proteins in the extracellular environment, it elucidates the interactions between cells and their surroundings. Secreted proteins are vital in processes such as development, immune responses, signal transduction, and disease progression. For instance, cytokines, hormones, and growth factors, as secreted proteins, are integral to imm......

• • Tandem Mass Spectrometry Protein Identification

  Tandem mass spectrometry protein identification is an essential technique in modern proteomics research, leveraging the strengths of mass spectrometry and protein chemistry to identify and quantify proteins within complex biological samples. Mass spectrometry analyzes the mass-to-charge ratio (m/z) of molecular ions to gather molecular information, while tandem mass spectrometry enhances this by providing detailed molecular structure insights through multi-stage analysis. In protein identification, ta......

• • Qualitative Analysis of Amino Acids and Proteins

  Qualitative analysis of amino acids and proteins is extensively applied in proteomics, metabolomics, and biomedical research. As fundamental building blocks of proteins, amino acids play crucial roles, with proteins serving as primary agents in virtually all biological processes. Proteins are indispensable in contexts ranging from cellular architecture and molecular signal transduction to enzymatic activity and immune response. The primary objective of qualitative analysis is to identify and character......

• • Shotgun Protein Identification

  Shotgun protein identification is a proteomics technique fundamentally reliant on mass spectrometry. It provides profound biological insights by thoroughly identifying and analyzing complex protein mixtures. Unlike traditional protein identification techniques, Shotgun does not depend on the prior separation and purification of proteins. Instead, it directly examines peptide mixtures generated from enzymatic digestion of protein samples. The methodology is characterized by its high throughput and sens......

• • SILAC-Based Mass Spectrometry

  SILAC-based mass spectrometry is a powerful tool for quantitative proteomics. SILAC involves the endogenous labeling of proteins by introducing stable isotope-labeled amino acids, such as ^13C or ^15N labeled lysine and arginine, into cell cultures. This results in proteins that exhibit specific isotope labels, which can be detected as mass differences in mass spectrometry analysis. SILAC-based mass spectrometry is primarily used for the relative quantification of proteins, playing a crucial role in i......