Resources

• • Application of Targeted Proteomics

  Targeted proteomics involves the precise measurement and analysis of predefined proteins in biological samples using specific methods, such as mass spectrometry. Compared to global proteomics, targeted proteomics emphasizes data accuracy and reproducibility. The process typically includes selecting target proteins, optimizing detection conditions, and validating results.

• • Advantages and Disadvantages of Targeted Proteomics

  Targeted proteomics has emerged as a pivotal tool in modern biomedical research, extensively employed for disease diagnosis, treatment, and biomarker discovery. By concentrating on specific proteins for detailed analysis, researchers can glean comprehensive information about biological samples.

• • Workflow of Targeted Proteomics

  Targeted proteomics is a method that employs specific mass spectrometry techniques to analyze predetermined proteins or peptide segments with high sensitivity and specificity. In contrast to global proteomics, targeted proteomics allows researchers to focus on a small number of specific targets, providing more precise data. The workflow generally includes the following key steps:

• • Principle of Targeted Proteomics

  Targeted proteomics differs from traditional shotgun proteomics, which typically involves analyzing all detectable proteins in a sample through mass spectrometry. In contrast, targeted proteomics focuses on pre-selected target proteins that are often relevant to specific biological issues or diseases.

• • Mechanism of Cellular Proteomics

  Cellular proteomics focuses not just on individual proteins but emphasizes the overall expression of proteins within a cell. By analyzing the abundance, variations, and interaction networks of proteins, it reveals the biological functions of the cell. Key technologies include Mass Spectrometry (MS), Protein Arrays, and bioinformatics.

• • Workflow of Cellular Proteomics

  Cellular proteomics is an important field that studies the composition, function, and interactions of all proteins within cells. By systematically analyzing cellular proteins, scientists can gain insights into biological processes and their roles in diseases.

• • Application of Cellular Proteomics

  As high-throughput technologies evolve rapidly, the field of cellular proteomics has revealed substantial potential across both fundamental research and clinical applications.

• • Principle of Cellular Proteomics

  Cellular proteomics is a crucial field focused on the composition, structure, function, and interactions of proteins within cells. 

• • Analysis of Methylated Proteins Using Orbitrap Fusion Lumos

  Methylation is a critical post-translational modification (PTM) of proteins that plays an essential role in regulating gene expression, cell signaling, and protein function. Protein methylation primarily occurs on lysine and arginine residues, and its dynamic changes are closely associated with the development of various diseases, such as cancer and neurodegenerative disorders.

• • Procedure of Quantitative Acetylomics Based on LC-MS/MS

  Protein acetylation is a common post-translational modification (PTM) that plays a crucial role in cellular function regulation. The dynamic regulation of acetylation is involved in various biological processes, including gene expression, cell cycle control, metabolic pathways, and signal transduction. Therefore, mapping the global acetylation landscape is essential for understanding intracellular regulatory mechanisms and the molecular basis of diseases.