Resources

Proteomics Databases



Metabolomics Databases

• • Mitochondrial Protein Sequencing

  Mitochondria are a unique type of organelle present within cells, serving as the primary source of energy within living organisms. They are responsible for several life-sustaining processes, including energy production, reducing free radicals, and maintaining cellular signaling. Proteins within the mitochondria play a key role in these processes.

• • Collagen Protein Detection Service Mass Spectrometry

  Collagen is the most abundant protein in animal tissues, accounting for one-third of the total protein content. Due to its crucial role in many biological processes, such as the structure and stability of the extracellular matrix, the analysis and detection of collagen have become an important area of research in the scientific field. In recent years, mass spectrometry has played a significant role in the detection and identification of collagen.

• • Protein-Protein Interaction Sequencing

  In-depth research on protein interaction plays a crucial role in life science research. Proteins are the main bearers of life activities, and their interactions form a complex molecular network within biological cells. Protein-Protein Interaction Sequencing (PPI-Seq) is an emerging technology. Based on high-throughput sequencing, it allows researchers to explore protein interactions at the whole genome level.

• • Nano Antibody Sequencing

  When we talk about antibody sequencing, we often think of issues related to disease treatment and improving human health. Nanobody sequencing provides us with a more precise and efficient solution. Nanobodies, also known as single-domain antibodies, are antibodies composed of a single functional domain.

• • How to Interpret De Novo Sequencing in Protein Mass Spectrometry?

  Protein sequencing by mass spectrometry is an effective way to analyze protein sequences. It is widely used in biological research, including disease research, drug discovery, and other areas. This article will focus on de novo protein sequencing by mass spectrometry and its spectrum resolution technology.   De novo protein sequencing is a method to directly obtain the amino acid sequence of a protein or peptide without relying on known protein sequence databases, using mass spectrometry techniques.

• • How Much Protein Is Needed for Edman Sequencing?

  In Edman protein sequencing, the amount of protein typically needed depends on various factors, such as the sensitivity of the sequencing equipment, the purity of the protein, and the complexity of the sequence. Traditionally, the Edman degradation method is used to determine the amino acid sequence of proteins or polypeptides, usually requiring about 1 to 100 picomoles (pmol) of protein sample.

• • How to Analyze Conserved Protein Sequences?

  The conserved protein sequences are sequences of proteins that remain unchanged or relatively stable throughout the evolution of species. These sequences usually play a crucial role in sustaining life activities. By analyzing conserved protein sequences, we can better understand their functions within organisms and their significance in species evolution. Below, we will introduce several commonly used methods for analyzing conserved protein sequences.

• • Mass Spectrometry Flow Protocol

  Materials and Equipment 1. Flow Mass Spectrometer 2. Samples 3. Flow Mass Spectrometry Reagent Kit 4. Polyurethane Filled Bottles 5. Sieve 6. Centrifuge 7. Pipette Tips 8. P1000 and P200 Pipettes 9. Melt Reagent Box 10. 1.5ml Centrifuge Tubes   Sample Processing 1. Melt the sample at room temperature. Transfer the sample to a new 1.5ml centrifuge tube. 2. Clean the sample with a cleansing solution. 3. Centrifuge the sample for 5 minutes at 10000rpm using a centrifuge. 4. Discard the supernatant.

• • What Does Protein Mass Spectrometry Generally Measure?

  Protein mass spectrometry detection is used to determine the mass, structure, and chemical modifications of proteins or polypeptides. Protein mass spectrometry detection is generally used for the following aspects:   1. Determination of Protein Mass By determining the mass of protein molecules, we can understand the relative molecular mass of proteins, which helps in protein identification.

• • Analysis of ADC Drug Biological Activity

  Antibody-drug conjugates (ADCs) are a new class of drugs that combine the advantages of both antibodies and small molecule toxins. Compared with traditional cytotoxins, ADCs have the advantages of strong targeting, less toxic side effects, etc. They can accurately target tumor cells, reduce side effects, and improve the therapeutic effect.   ADC drugs are mainly composed of monoclonal antibodies, linkers, and small molecule cytotoxins.