Resources

Proteomics Databases



Metabolomics Databases

• • Advantages and Disadvantages of Far-Western Blot in Protein-Protein Interaction Studies

  Protein-protein interactions (PPI) are essential for understanding cellular functions, signal transduction, and disease mechanisms. Far-Western blotting is a powerful technique that enables the detection of interactions between proteins. This technique employs a known protein as a probe, which binds specifically to a target protein, thereby facilitating the identification of protein-protein interactions within complex protein mixtures.

• • Principle of Far-Western Blot in Protein-Protein Interaction Analysis

  Far-Western blotting is a widely utilized technique for investigating protein-protein interactions. Unlike traditional immunoblotting, Far-Western blotting employs a protein probe instead of an antibody to detect target proteins in a sample. This technique provides a critical method for uncovering molecular mechanisms in complex biological processes, allowing the study of protein interactions under non-denaturing conditions that closely mimic physiological states.

• • Kelp Histones Glycoform Analysis

  Kelp, as a representative of large brown algae, is one of the most important economic seaweeds in the world. Apart from its applications in food and industry, kelp is also a source of many valuable bioactive substances, one of which is its histone. Histone in organisms often bind with sugar molecules to form histone-glycan conjugates, and these sugar molecules can affect the function and stability of glycoproteins. Therefore, the analysis of the glycoform of kelp histone is critical.

• • AUC Analysis of Polypeptide Drugs by Ultracentrifugation

  Peptide drugs are bioactive molecules formed by connecting multiple amino acids through peptide bonds. Generally, they are formed by connecting 10-100 amino acids, with a relative molecular mass lower than 10000. Most peptide drugs come from endogenous peptides or natural peptides, so they have no or little side effects on the human body. Compared to protein drugs, peptide drugs also have advantages such as good stability, high purity, low production cost, and low immunogenicity.

• • Capillary Isoelectric Focusing (CIEF) Analysis of Peptide Drugs

  Peptide drugs are bioactive molecules composed of multiple amino acids connected by peptide bonds. Typically, they are made up of 10~100 amino acids, with a relative molecular mass less than 10,000. Most peptide drugs are derived from endogenous peptides or natural peptides, so they have little or no side effects on the human body. Compared with protein drugs, peptide drugs also have advantages such as good stability, high purity, low production cost, and low immunogenicity.

• • Q-TOF Peptide Map Analysis Steps

  The Q-TOF (Quadrupole-Time of Flight) mass spectrometer is a mass spectrometry device that combines quadrupole and Time of Flight (TOF) technologies. It combines the selective ion transmission of quadrupole mass spectrometry and the high resolution, high precision measurement capabilities of Time of Flight mass spectrometry. It is often used for peptide mass spectrometry analysis, especially in protein identification and proteomics research.

• • De Novo Sequencing of Nasopharyngeal Carcinoma Tissue

  Nasopharyngeal carcinoma, a malignant tumor located in the head and neck, has a complex pathogenesis closely related to factors such as genetics, environment, and viral infections. Due to its hidden location and diverse clinical manifestations, it brings certain challenges to its early diagnosis and the choice of treatment plan. In this context, it is particularly important to perform de novo sequencing of nasopharyngeal carcinoma tissues.

• • Tripeptide Sequences: The Biological Code of the Microcosm

  In the broad field of biology, proteins are renowned for their unique and diverse functions. The driving forces behind these functions are often short amino acid sequences in proteins, such as tripeptides. These small fragments consisting of three amino acids, though small, play a key role in processes such as signal transduction, protein interactions, and metabolic regulation.

• • De Novo Sequencing: Unveiling Unknown Peptides

  "De novo" sequencing is a technique in bioinformatics mainly used to determine the amino acid sequences of new or unknown proteins or peptides. Compared to re-sequencing, this technique pays special attention to those proteins or peptides that have not been identified or are not present in existing databases.

• • Secretory Protein Sequencing: Decoding Extracellular Proteins

  Secretory proteins are those proteins that are released to the outside of the cell through the cell's secretion pathway. They play a key role in various biological processes, such as immune responses, cell signaling, inflammatory responses, etc. The sequencing of secretory proteins is to determine their amino acid sequence, thereby understanding their function, structure, and interactions with other proteins or small molecules.