Resources

Proteomics Databases



Metabolomics Databases

• • IP Experiment Guide: Protein Structure Analysis

  Protein is one of the most basic functional molecules in living organisms, and its structure plays a crucial role in its function. The identification of protein structure is one of the important topics in the research field of biological drugs. This article will introduce the basic principles and common experimental steps of protein structure analysis, helping readers to better understand the structure of proteins and their applications in biological drug research.

• • Kidney Tissue Histone Post-Translational Modification Analysis

  The kidney, as the main excretion and regulation organ of the human body, carries a multitude of physiological tasks. In its complex physiological and pathological processes, post-translational modifications (PTMs) of histones play a crucial and indispensable role.

• • Mechanism of TMT Labeling in Mass Spectrometry

  Tandem Mass Tag (TMT) quantitative proteomics analysis is a widely employed technique in biological research for the quantitative analysis of proteins. The core principle of TMT involves chemically tagging peptides from different samples, followed by high-throughput and precise quantification using tandem mass spectrometry (MS/MS). This technique's strength lies in its ability to analyze multiple samples simultaneously with high sensitivity and accuracy, making it indispensable in biomedical research.

• • Application of TMT-Based Quantitative Proteomics in Biomarker Discovery

  TMT (Tandem Mass Tags) quantitative proteomics analysis is a mass spectrometry-based technique for protein quantification, extensively applied in biomedical research, disease mechanism exploration, drug development, and other fields. By introducing stable isotope labels into protein samples, TMT technology enables the relative quantification of protein expression levels across different samples.

• • Workflow of TMT-Based Quantitative Proteomics Analysis

  Tandem Mass Tag (TMT) quantitative proteomics analysis is a powerful technique widely used in the comparative analysis of multiple samples. By labeling samples with specific isotopic tags, TMT technology enables the simultaneous quantification of up to 16 samples. This method not only increases the throughput of the experiment but also reduces variability between samples.

• • Advantages and Disadvantages of TMT-Based Quantitative Proteomics Analysis

  TMT (Tandem Mass Tags)-based quantitative proteomics analysis is a mass spectrometry-driven high-throughput technique designed to simultaneously compare the relative expression levels of proteins across multiple samples. By covalently attaching TMT labels to peptides, researchers can achieve multiplexed sample labeling and perform relative quantification during mass spectrometry analysis. This method is widely used in proteomics, particularly for studies requiring comparisons across multiple sample groups.

• • Principle of TMT-Based Quantitative Proteomics Analysis

  TMT (Tandem Mass Tags) quantitative proteomics analysis is a highly sensitive, multiplexed quantification technique based on mass spectrometry, widely used in proteomics research. This technology enables the relative quantification of multiple samples by labeling them with distinct tags and analyzing their intensities through mass spectrometry.

• • Mechanism of Far-Western Blot in Protein-Protein Interaction Detection

  Far-Western blotting is a powerful technique for detecting protein-protein interactions. Unlike traditional Western blotting, which relies on antibodies, Far-Western blotting employs a protein probe to identify target proteins. This technique is indispensable in studying protein interactions, elucidating signal transduction pathways, and discovering novel drug targets.

• • Workflow of Far-Western Blot for Detecting Protein-Protein Interactions

  Far-Western blotting is a crucial technique for studying protein-protein interactions. Unlike traditional Western blotting, this method leverages the binding capabilities of a known protein to identify target proteins, making it particularly useful for detecting specific ligand-binding proteins within complex mixtures. Its applications are significant in research areas such as signal transduction pathways and protein complex analysis.

• • Application of Far-Western Blot in Protein Binding Affinity Studies

  Protein-protein interactions (PPIs) are pivotal in biological research, influencing essential processes such as intracellular signal transduction, metabolic regulation, and gene expression. They also provide profound insights into disease mechanisms. Consequently, methods to investigate protein-protein interactions are highly valuable in life sciences.