Resources
Proteomics Databases
Metabolomics Databases
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• Antibody Characterization Assays
Antibody characterization assays involve comprehensive analyses to determine the structural, functional, and other key properties of antibodies. As crucial components of the immune system, antibodies recognize and neutralize foreign pathogens, such as viruses and bacteria. These assays enable researchers and clinicians to evaluate binding specificity, affinity, stability, and purity, properties that directly influence antibody functionality and efficacy. During the development of antibody-based drugs ......
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• Antibody Characterization Services
Antibody characterization is primarily used to identify, analyze, and verify the properties of antibodies and their functions in biological systems. As key components of the immune system, antibodies are widely utilized in disease diagnosis, treatment, drug development, and fundamental scientific research. Antibody characterization relies on the specific binding reactions between antigens and antibodies, wherein an antibody binds exclusively to a specific epitope on an antigen to form an antigen-antib......
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• 2D Gel Electrophoresis and Mass Spectrometry
2D gel electrophoresis and mass spectrometry are two pivotal technologies in proteomics research that complement each other to provide a robust platform for protein identification and quantitative analysis. Researchers in proteomics often face the challenge of analyzing complex protein mixtures, where a single technique cannot meet the dual demands for high resolution and throughput. 2D gel electrophoresis efficiently separates proteins on a two-dimensional plane based on isoelectric points (pI) and m......
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2D protein analysis is a high-resolution separation technique extensively used in proteomics to comprehensively analyze protein composition, expression levels, and post-translational modifications (PTMs) in complex biological samples. The method combines two distinct separation mechanisms: isoelectric focusing (IEF) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In the first dimension, IEF separates proteins based on their isoelectric points (pI) along a stable pH gradient. ......
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Single cell mRNA sequencing is a revolutionary gene expression analysis technology that profiles mRNA expression at the single-cell level, enabling the exploration of cellular heterogeneity and dynamic changes under various biological states. This technique provides transformative insights into cell function, developmental processes, disease mechanisms, and intercellular communication, with wide-ranging applications in tumor research, immunology, neuroscience, and stem cell studies. Tumor tissues, com......
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2D gel analysis is a high-resolution method for separating and identifying proteins, widely utilized in proteomics research. This technique integrates two electrophoresis methods-soelectric focusing (IEF) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-to separate protein samples on a two-dimensional plane. In the first dimension, proteins are separated based on their isoelectric points (pI) through IEF. In the second dimension, SDS-PAGE resolves proteins according to their m......
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• PPI Protein-Protein Interaction
PPI Protein-Protein Interaction refers to the stable or transient associations between two or more protein molecules mediated by specific binding sites and non-covalent forces, such as hydrogen bonds, electrostatic interactions, and hydrophobic effects. These interactions are fundamental to cellular functions, underpinning nearly all physiological and pathological processes, including signal transduction, gene regulation, cell cycle control, metabolic pathways, and immune responses. PPIs can be classi......
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• Protein-Protein Interaction Mass Spectrometry
Protein-protein interaction mass spectrometry is a robust technique for investigating protein interaction networks using advanced mass spectrometry approaches. Protein-protein interactions (PPIs) underpin critical cellular processes, including signal transduction, cell cycle regulation, gene expression, metabolic control, and immune responses. These interactions vary dynamically between physiological and pathological states, with aberrant PPIs closely linked to diseases such as cancer, neurodegenerati......
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• Protein Sequencing by Edman Degradation
Protein Sequencing by Edman Degradation is a well-established method for determining the amino acid sequences of proteins. Developed in 1950 by the Swedish biochemist Pehr Edman, this technique enables stepwise sequencing by cleaving and labeling the N-terminal amino acids of a protein. Specifically, phenyl isothiocyanate (PITC) reacts with the N-terminal amino acid to form a phenylthiohydantoin derivative, which is selectively cleaved under acidic conditions to release a cyclic compound. This derivat......
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Peptide characterization is a fundamental aspect of proteomics research, focusing on the comprehensive analysis of peptide structures, sequences, properties, and functions. Peptides, which are small molecular fragments typically composed of 2 to 50 amino acids linked by peptide bonds, are derived from protein degradation. Despite their simpler structures compared to intact proteins, peptides exhibit diverse and complex biological functions, including roles in signal transduction, immune regulation, an......
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