Resources

• • Mechanism of Protein Separation in Serum Proteomics

  Serum proteomics is an essential field for studying the composition and function of proteins in serum. Due to the large number of proteins present in serum, its complexity poses challenges for analysis. An effective protein separation mechanism is fundamental for conducting proteomic studies, establishing a solid foundation for subsequent quantitative analysis, identification, and functional research.

• • Workflow of Serum Proteomics for Disease Biomarker Discovery

  Serum proteomics is the study of the composition and changes of proteins in serum, which has been widely applied in the discovery of disease biomarkers in recent years. Biomarkers are essential tools for disease diagnosis, prognosis evaluation, and treatment monitoring. Serum proteomics, through high-throughput technologies, can provide a large amount of quantitative and qualitative data about proteins.

• • Mechanism of Protein-Lipid Interactions in Membrane Proteomics

  Membrane proteomics investigates the composition, structure, and functions of proteins in cell membranes, focusing on the interactions between membrane proteins and lipids. Membrane proteins are crucial in cell signaling, substance transport, and energy conversion. Protein-lipid interactions not only influence the conformation and functionality of membrane proteins but also regulate the physicochemical properties of cell membranes.

• • Application of Membrane Proteomics in Subcellular Analysis

  Membrane proteomics is a vital field dedicated to studying the proteins located on cell membranes and their interactions. It has a wide range of applications, particularly in subcellular analysis. By employing membrane proteomics, scientists can gain deeper insights into the functions of various cellular membranes, revealing the critical roles that membrane proteins play in signal transduction, material transport, and cell recognition.

• • Advantages and Disadvantages of Mass Spectrometry-based Membrane Proteomics

  Membrane proteins are crucial biomolecules located on cell membranes, playing key roles in various physiological processes such as cell signaling, substance transport, and immune responses. Given the significance of membrane proteins in biological and medical research, membrane proteomics has become an area of great interest. Mass spectrometry (MS), as a powerful analytical tool, has demonstrated unique advantages and challenges in membrane proteomics.

• • Workflow of Nano-LC-MS in Membrane Proteomics

  Membrane proteomics is a vital field in biological and medical research, focusing on the structure, function, and roles of membrane proteins within cells. Membrane proteins play key roles in cell signaling, substance transport, and cellular recognition. Due to their often low concentrations in complex biological samples, high-sensitivity analytical techniques are required for their study.

• • Principle of Membrane Proteomics in Biological Systems

  Membrane proteomics is an essential branch of biological research focused on studying membrane proteins and their functions within biological systems. Membrane proteins play crucial roles in cellular functions, signal transduction, and material transport. Understanding the principles of membrane proteomics aids in unraveling the complexity of biological systems and their regulatory mechanisms.

• • Precise Quantitative: Label-free Protein Analysis

  Accurate quantification of proteins is vital in biomedical research. In recent years, the development of label-free protein analysis technology has provided a new option for precise quantification. Traditional protein quantification methods require the use of markers, such as fluorescent dyes or isotopic labels, but these methods have some limitations.

• • Peptide Sequencing: Advanced Techniques for Amino Acid Arrangement

  Peptides are formed by connecting amino acids through peptide bonds. Peptide sequencing refers to determining the specific order of amino acids in a peptide or protein. This information is crucial for understanding the function, structure, and interaction of peptides with other molecules.

• • Analysis of Characteristic Peaks of Lysine in Circular Dichroism Spectroscopy

  Circular dichroism (CD) is a technique used to study the secondary and tertiary structures of proteins, nucleic acids, and other biomolecules. By measuring the molecular rotation at specific wavelengths, information about their structures can be obtained.