Resources

Proteomics Databases



Metabolomics Databases

• • Linear Ubiquitination Quantitative Mass Spectrometry

  Linear ubiquitination is an important protein modification in organisms that regulates various biological processes, including immune response, inflammatory response, cell signaling, and DNA damage repair. However, due to its uniqueness and complexity, the study of linear ubiquitination has faced technical challenges in the past. Fortunately, recent technological advancements have allowed us to investigate linear ubiquitination in more depth.

• • How to Detect the Quantity of Cell Surface Glycoproteins?

  Cell surface glycoproteins play a crucial role in many cellular processes, including cell adhesion, immune response, and signal transduction. Therefore, it is important to understand how to detect the quantity of cell surface glycoproteins in order to study their functions and regulation.

• • Is Histone Methylation Part of Proteomics?

  Histone methylation belongs to proteomics, but it leans more towards epigenetics. Histones are structural proteins of eukaryotic chromosomes and are a class of small, basic proteins. Histone methylation refers to the addition of one, two, or three methyl groups to certain amino acids in histones, and it is an important histone modification.

• • Infrared Spectroscopy for Protein Secondary Structure Analysis

  The secondary structure of a protein refers to the specific conformation formed by the peptide backbone atoms, which can be either in a helical or folded arrangement along a certain axis. It does not involve the side chains of the amino acid residues. The main types of protein secondary structure include α-helix, β-sheet, β-turn, and random coil, with hydrogen bonds playing a major role in maintaining these structures.

• • Chiral Circular Dichroism Spectrum

  Chirality is one of the fundamental physical and chemical properties in nature. Chiral symmetry phenomena typically represent an important symmetric characteristic, namely mirror symmetry, in the field of physical chemistry research. If an object cannot completely overlap with its mirror image, it is considered to possess chirality, also known as handedness. We refer to this kind of molecule that cannot be superimposed onto its mirror image as a chiral molecule.

• • Hydrogen-Deuterium Exchange Mass Spectrometry Experiment

  With the development of biopharmaceutical technology, proteins are gradually being applied in various clinical indications as main targets or therapeutic agents in drug research. The structure of proteins is dynamic, and it can change with post-translational modifications, ligand binding, or interactions between proteins. Therefore, studying the conformational changes of proteins in the drug design process is crucial for understanding the mode of action and function of candidate drugs.

• • ECD Circular Dichroism Spectroscopy

  Research on chiral molecules has always held an important position in the fields of physics, chemistry, and biology. Circular dichroism (CD), as a powerful spectroscopic technique, reveals the spatial structure and conformation of molecules by measuring the difference in absorption of chiral molecules to left- and right-circularly polarized light. Circular dichroism can be divided into two types: electronic circular dichroism (ECD) and vibrational circular dichroism (VCD).

• • Detection of Free Thiol Content

  The free thiol, also known as a thiol group or sulfhydryl group, is the -SH group at the end of the cysteine residue side chain. It is highly reactive and can be oxidized by oxidizing agents to form various thiol modifications. The chemical properties of free thiols are very active and can bind to proteins to change their structure and function, or react with oxygen molecules to cause oxidative damage to cells and tissues.

• • How to Detect mRNA Capping Efficiency?

  Messenger RNA (mRNA) is a nucleotide sequence that encodes proteins and consists of five main parts: the 5' cap structure, the 5' untranslated region (UTR) that is associated with translation efficiency, the open reading frame (ORF) region that encodes the protein, the 3' UTR that is associated with translation efficiency, and the poly-A tail composed of multiple adenosine monophosphates, which is related to mRNA stability and translation efficiency.

• • Phosphorylation Modification Proteomics

  Phosphorylation-based modomics is a discipline that focuses on the comprehensive study of phosphorylation modifications in organisms. In the fields of biochemistry and molecular biology, phosphorylation is a critical post-transcription protein modification, playing a core role in regulating protein function, cell signal transmission, metabolic pathways, and various cellular processes.