Resources
Proteomics Databases
Metabolomics Databases
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HCP impurity refers to non-target proteins secreted or released by host cells during the production of biopharmaceuticals, including recombinant proteins, monoclonal antibodies, and gene therapy products. Although HCP impurity are typically present in trace amounts and may have minimal effects on the product's activity or stability, their detection and removal are critical during purification and quality control due to their potential immunogenicity, which could compromise patient safety. Therefore, s......
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• Charge Variant Analysis of Monoclonal Antibodies
Charge variant analysis of monoclonal antibodies (mAbs) is a vital technique in proteomics, primarily used to detect and characterize charge variants in monoclonal antibodies. These charge variants arise from chemical modifications or mutations in the amino acid residues of the antibody structure, leading to shifts in positive or negative charges. Such changes may result from minor variations in manufacturing processes, chemical modifications (e.g., glycosylation, deamidation, oxidation), or protein d......
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• HCP Quantification in Biopharmaceutical Development
HCP quantification is a critical process in biopharmaceutical development, focusing on the detection and quantification of host cell proteins that may be present as impurities in biologic drugs. These non-target impurities, expressed by host cells during the production of recombinant proteins and other biologics, can potentially trigger immune responses and adversely affect drug safety and efficacy. Therefore, they must be rigorously monitored and effectively removed during drug development. The prima......
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Polysaccharide sequencing is an analytical technique used to determine the monosaccharide composition, linkage patterns, and branching structures of polysaccharide molecules. In the fields of biochemistry and molecular biology, polysaccharides are known for their structural complexity and functional diversity, which makes sequencing essential for understanding their roles in biological systems. Polysaccharides are crucial in cellular architecture, energy storage, signal transduction, and biological pr......
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Host cell DNA, the genetic material present within host cells, holds significant importance in the field of biology. It encompasses all the genetic information necessary for guiding the growth, division, and functionality of an organism's cells. Structurally, this DNA is composed of a double helix formed by four nucleotides: adenine, guanine, cytosine, and thymine, connected by phosphodiester bonds into long chains. The coding sequences of genes within the DNA influence protein synthesis in host cells......
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MS/MS peptide sequencing, or tandem mass spectrometry peptide sequencing, is a pivotal technique in proteomics research. Given that proteins are fundamental to life functions, any alteration in their structure or function can significantly impact an organism's health and disease states. This technique involves ionizing protein samples with a mass spectrometer and fragmenting them into peptides through sequential mass spectrometry analysis. By examining the mass of these fragments, researchers can reco......
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• N- and C-Terminal Sequencing
N- and C-terminal sequencing are techniques used to analyze the amino acid sequences at the amino (N-terminal) and carboxyl (C-terminal) ends of protein molecules. Proteins, composed of amino acids linked by peptide bonds, possess distinct biological functions. The sequences at their N- and C-terminals are crucial in defining their structural and functional attributes. The N-terminal sequence begins with a free amino group, whereas the C-terminal sequence concludes with a free carboxyl group. By exami......
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• PTM Analysis by Mass Spectrometry
PTM analysis by mass spectrometry is a powerful technique in proteomics that provides detailed insights into the complex regulatory mechanisms of proteins. Post-translational modifications (PTMs) refer to covalent changes that occur after protein synthesis, such as phosphorylation, glycosylation, and acetylation. These modifications can profoundly influence protein function, stability, and cellular localization. Thanks to its high sensitivity, resolution, and throughput, mass spectrometry has become t......
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• Protein Target Identification
Protein target identification is an essential step in proteomics research, aimed at identifying and validating proteins involved in specific biological processes, disease states, or drug interactions. Protein targets are those proteins that perform specific functions within and outside cells and may act as critical regulators in diseases or as direct drug targets. By elucidating the relationships between these proteins and their biological functions, scientists gain a deeper insight into cellular mech......
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• Proximate Analysis of Protein
Proximate analysis of protein refers to the process in proteomics research that employs a range of bioinformatics and experimental techniques to compare and analyze similar protein sequences or structures, with the aim of uncovering their biological functions, evolutionary relationships, and structural characteristics. This approach involves evaluating sequence similarity, structural similarity, and functional similarity. Proximate analysis of protein is commonly used to predict the function of unknow......
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