Resources
Proteomics Databases
Metabolomics Databases
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HCP coverage analysis is a key technique for detecting and quantifying residual host cell proteins (HCPs) in biopharmaceutical products. During the production of therapeutic proteins, host cells such as E. coli, yeast, or mammalian cells are commonly used. However, these cells may release or secrete various non-target proteins, collectively known as HCPs, during production. The presence of HCPs can compromise drug safety and efficacy, necessitating accurate detection and analysis throughout biopharmac......
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• HPLC Method for Protein Analysis
HPLC method for protein analysis is a robust and versatile technique used to separate protein components in a sample by employing a liquid mobile phase that flows through a chromatography column. This approach facilitates the fractionation of proteins based on specific properties such as size, hydrophobicity, and charge. Compared to traditional analytical methods, HPLC method for protein analysis offers significant advantages, including faster analysis and improved detection precision and accuracy. Re......
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Aptamer-based proteomics involves the analysis of protein composition and abundance through the specific recognition and binding capabilities of nucleic acid aptamers. These aptamers, composed of DNA or RNA sequences, are identified using SELEX (Systematic Evolution of Ligands by EXponential enrichment)—an in vitro selection process that isolates nucleic acid sequences with high affinity and specificity for target proteins from a vast library. Compared to traditional antibody-based detection methods......
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• Edman Degradation Protein Sequencing
Edman degradation protein sequencing is a classical technique for protein sequence analysis that facilitates the precise determination of protein amino acid sequences through sequential removal of amino acid residues. Developed in the 1950s by Swedish chemist Pehr Edman, this method has become an indispensable tool in proteomics research. The core principle of Edman degradation involves the selective binding of specific chemical reagents to the amino terminus of a protein, followed by stepwise removal......
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SEC-MALS analysis is an advanced technique that combines Size Exclusion Chromatography (SEC) and Multi-Angle Light Scattering (MALS) to determine the absolute molecular weight, molecular size, and aggregation state of biomolecules. This method is widely employed in fields such as structural biology, proteomics, and biopharmaceuticals. The principle of SEC-MALS analysis involves separating sample components based on molecular size using SEC, followed by real-time analysis of the eluted fractions via an......
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• Proteomics Identify Differential Proteins
Proteomics is a scientific approach focused on studying the protein composition and functionality within biological systems. Differential protein analysis is a key method for assessing variations in protein expression levels under different experimental conditions, such as comparing control and experimental groups. The following are standard methodologies for differential protein identification:
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• Protein Identification by Small Molecule Mass Spectrometry
Protein small molecule identification using mass spectrometry is a technique employed to analyze and determine the composition and structure of proteins or peptides. This method primarily operates by measuring molecular mass and the mass-to-charge ratio (m/z), constituting a pivotal technology in proteomics research. The fundamental steps involved are as follows:
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• Quantitative Detection of Different Proteins Within a Cell
Quantitative detection of different proteins within cells is a crucial aspect of biological and biomedical research. It aims to understand changes in protein expression levels, protein networks, and their roles in cell function and disease.
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• Spatial Proteomics Metal Tag
Spatial Proteomics with Metal Tags is an advanced method that combines mass spectrometry and bioimaging technologies to study the spatial distribution and function of proteins at the tissue or cellular level. This technique involves labeling proteins with heavy metal ions, followed by imaging using Laser Ablation Mass Spectrometry Imaging (LA-MSI) or similar techniques. It enables precise analysis and visualization of protein distribution in biological tissues without damaging the samples.
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• Chemical Proteomics and Drug Target Discovery
Chemical proteomics is a research approach that combines chemistry, biochemistry, and proteomics techniques. It is primarily used to study the interactions between proteins and small molecules, thereby revealing the functions and regulatory mechanisms of proteins, as well as their roles in diseases. The main steps include:
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