Resources
Proteomics Databases
Metabolomics Databases
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• How to Identify Differences Between Two Groups of Proteins Through Proteomics Research
The main steps involved in identifying differences between two groups of proteins through proteomics research include the following: Sample Preparation: Select two or more different conditions or sample groups (e.g., disease group and control group). Extract proteins and conduct necessary processing, such as protein concentration measurement and normalization. Protein Separation and Identification: Two-dimensional gel electrophoresis (2-DE) is a traditional protein separation technique that achieves......
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• Protein Chemistry and Proteomics
Protein chemistry and proteomics are two important fields that study biomolecules, particularly proteins. They explore the structure, function, and interactions of proteins from different perspectives, providing a foundation for understanding biological processes and disease mechanisms. Although these fields are closely related, they differ in their focus and the technical methods they use.
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• Antibody-Drug Conjugate Analysis
Antibody-Drug Conjugate Analysis refers to the process of in-depth study and evaluation of the structure, composition, and characteristics of antibody-drug conjugates (ADCs). ADCs represent a novel class of biotherapeutic agents that combine cytotoxic drugs with specific antibodies, enabling targeted delivery of the drug to cancer cells and achieving efficient tumor-killing effects. This strategy not only enhances the therapeutic index but also minimizes toxicity to healthy cells. The primary objectiv......
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• Antibody Sequencing Service for Drug Development
Antibody Sequencing Service refers to the process of determining the amino acid sequence of an antibody through high-precision mass spectrometry analysis and bioinformatics techniques. Antibodies are critical molecules of the immune system, playing an essential role in defending against pathogens by recognizing and binding to specific antigens. Antibody sequencing services have wide-ranging applications in the biopharmaceutical field, including but not limited to antibody drug development, antibody en......
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2D-DIGE proteomics, or two-dimensional difference gel electrophoresis proteomics, is a high-resolution protein separation technique derived from traditional two-dimensional gel electrophoresis (2-DE). By combining fluorescence dye labeling, it allows for the simultaneous analysis of multiple samples. In 2D-DIGE, protein mixtures are separated via isoelectric focusing and SDS-PAGE, enabling both resolution and quantification of samples on the same gel. This technique uniquely labels samples with differ......
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Antibiotic identification refers to the scientific techniques used to detect and confirm the types and characteristics of antibiotics. Antibiotics are secondary metabolites produced by microorganisms (such as bacteria and fungi) that can inhibit or kill other microorganisms. They are extensively used in both medical and agricultural applications. Antibiotics encompass a wide range of compounds, including penicillins, cephalosporins, aminoglycosides, and tetracyclines, each with distinct mechanisms of ......
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• SDS-PAGE Analysis of Proteins
SDS-PAGE analysis of proteins is a widely employed technique in biochemical and molecular biology research, designed for separating and analyzing individual proteins within complex mixtures. SDS-PAGE, which stands for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, leverages the denaturing properties of SDS (Sodium Dodecyl Sulfate) and the resolving power of polyacrylamide gels to separate proteins based on their molecular weight. SDS, an anionic detergent, binds uniformly to proteins, mask......
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SDS-PAGE protein separation is a widely utilized electrophoretic technique in proteomics, enabling the separation of proteins based on their molecular weight. SDS, or Sodium Dodecyl Sulfate, is an anionic detergent that denatures proteins by disrupting their secondary and tertiary structures, ensuring uniform linear migration during electrophoresis. PAGE, short for Polyacrylamide Gel Electrophoresis, employs a gel matrix to facilitate size-based separation. In this method, protein samples are treated ......
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• Proximity Labeling Proteomics
Proximity labeling proteomics is an advanced biochemical approach designed to map protein spatial relationships and dynamic interactions within cells. By genetically fusing specific enzymes such as biotin ligase BirA or peroxidase APEX to target proteins, these enzymes can selectively label proximal proteins with biotin tags. The biotinylated proteins are subsequently enriched via streptavidin affinity purification and analyzed using high-resolution mass spectrometry for identification and quantificat......
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mRNA analysis is a method for investigating messenger RNA (mRNA) molecules, which serve as essential intermediaries in gene expression by conveying genetic instructions from DNA to the protein synthesis machinery. This technique primarily aims to determine gene expression levels and patterns, providing insights into the regulation of genes under various conditions. By examining mRNA expression, researchers can pinpoint disease-related genes, identify critical regulatory nodes in biological pathways, a......
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