Resources

Proteomics Databases



Metabolomics Databases

• • Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry

  Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) is a powerful analytical technique extensively used in proteomics, chemistry, drug development, and clinical diagnostics. Its principle relies on the absorption of laser energy by matrix molecules, enabling the desorption and ionization of sample molecules. The flight time of these ions is then measured by a time-of-flight mass spectrometer to determine their mass. The efficiency of this technique largely depen......

• • Enzyme Identification

  Enzyme Identification refers to the identification and characterization of enzymes in biological samples through a series of experimental and analytical approaches. Enzymes are proteins that serve as catalysts for biochemical reactions, playing critical roles in cellular metabolism, signal transduction, and gene expression regulation. Understanding enzyme types and functions is essential for elucidating biological mechanisms, developing novel therapeutics, and advancing biotechnological applications. ......

• • Protein Domain Analysis

  Protein domain analysis is an essential component of protein research. Protein domains are distinct structural and functional units within protein macromolecules, capable of existing in a stable state and performing specific functions independently. In the drug development field, protein domain analysis is widely applied in the screening and design of drug targets. By analyzing protein domains, researchers can elucidate the binding mechanisms between drug molecules and protein targets, facilitating th......

• • Protein Expression Analysis

  Protein expression analysis is an analytical technique used to investigate the levels and dynamic changes of proteins within organisms. By examining the expression levels and states of proteins in cells or tissues, this technique provides insights into their functional roles in biological processes. Through protein expression analysis, researchers can elucidate the molecular mechanisms underlying various biological phenomena, such as cell differentiation, signal transduction, and immune responses. In ......

• • MS/MS Sequencing

  MS/MS sequencing is a powerful technique for determining amino acid sequences in complex protein mixtures. This method employs two stages of tandem mass analyzers to analyze peptides. Proteins are first digested into smaller peptide fragments, and a specific peptide is selected by the first mass analyzer. The peptide is then fragmented further in a collision cell, with the second mass analyzer measuring the mass-to-charge ratios of the resulting fragments. By interpreting this fragment data, the amino......

• • N-Terminal Sequencing Analysis

  N-terminal sequencing analysis is a pivotal technique in proteomics used to determine the N-terminal amino acid sequence of proteins or peptides. This sequence provides critical insights into the biochemical properties, biological functions, and evolutionary relationships of proteins, which are fundamental to life processes. In the biopharmaceutical industry, N-terminal sequencing analysis plays an integral role in drug development and quality assurance. It verifies the N-terminal sequence of recombin......

• • LC-MS/MS Protein Sequencing

  LC-MS/MS protein sequencing is a powerful analytical technique for identifying and characterizing proteins in complex samples. This method begins with liquid chromatography to separate protein mixtures into simpler components based on physical and chemical properties such as molecular weight and hydrophilicity. The separated proteins are then ionized and analyzed using tandem mass spectrometry, which measures the mass-to-charge ratios of fragmented peptides, providing detailed sequence information.   ......

• • De Novo Protein Sequencing Mass Spectrometry

  De novo protein sequencing mass spectrometry is a cutting-edge technique for directly determining the primary structure of proteins. This approach is particularly valuable for samples lacking genomic data or for identifying unknown modifications, variations, or processing sites in known sequences. Unlike traditional sequencing methods that rely on genomic or transcriptomic databases for alignment, de novo protein sequencing mass spectrometry derives amino acid sequences directly from experimental data......

• • Glycan Profiling of Monoclonal Antibodies

  Glycan profiling of monoclonal antibodies is a specialized analytical method for studying the glycosylation patterns attached to monoclonal antibodies. These antibodies, a cornerstone of modern biopharmaceuticals, play critical roles in the treatment of cancer, autoimmune disorders, and infectious diseases. However, their functionality and stability are not solely determined by their amino acid sequences but are also significantly influenced by glycosylation modifications. Glycan modifications, the su......

• • Circular Dichroism Secondary Structure Analysis

  Circular dichroism secondary structure analysis is a spectroscopic technique used to study the secondary structure of proteins. By measuring the differential absorption of circularly polarized light, circular dichroism (CD) provides precise information about protein secondary structures in solution, including α-helices, β-sheets, β-turns, and random coils. Widely utilized in biochemical and molecular biology research, circular dichroism secondary structure analysis enables rapid assessment of protein ......