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    Resources

      Proteomics Databases

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      Metabolomics Databases

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    • • Advantages and Limitations of De Novo Protein Sequencing

      De novo protein sequencing is an advanced technique for determining protein sequences without requiring genomic data or database references. This approach is particularly valuable for studying unknown proteins, novel antibodies, non-model organisms, and complex post-translational modifications (PTMs). Unlike traditional mass spectrometry-based identification, which relies on database searches, de novo sequencing is especially useful for novel protein discovery and modification analysis. However, chall......

    • • How Does Native MS Revolutionize Structural Biology?

      Native MS is an advanced analytical technique that enables the characterization of protein mass, assembly states, and molecular interactions under near-physiological conditions. As structural biology expands from single-protein studies to complex, dynamic multiprotein systems, native MS has emerged as a transformative and complementary approach that enhances conventional structural biology methods. This review systematically examines the critical role of native MS in structural biology, highlights its......

    • • Flow Injection Analysis Mass Spectrometry

      Flow injection analysis mass spectrometry (FIA-MS) combines the strengths of flow injection analysis and mass spectrometry, enabling rapid and precise qualitative and quantitative analysis of chemical components in complex samples. The underlying principle of FIA-MS is relatively straightforward. In this method, a liquid sample is introduced into the injection valve through a flow injection system, where it mixes with a carrier solvent before entering the mass spectrometer under controlled conditions.......

    • • Receptor Identification and Characterization

      Receptor identification and characterization techniques are extensively utilized in modern biological research fields, including drug development, disease studies, and fundamental biological investigations. Receptors, which are proteins located on the cell surface or within cells, are responsible for detecting and binding specific signaling molecules-such as hormones, neurotransmitters, and drugs-thereby initiating biological effects. Within biological systems, receptors function akin to switches, con......

    • • Protein Primary Structure Analysis

      Protein primary structure analysis is a fundamental step in protein research, focusing on determining and understanding the amino acid sequence of proteins. As essential functional molecules within organisms, proteins perform a wide range of biological activities. Their primary structure, the specific order of amino acids, is crucial for elucidating their function and properties. Analyzing this structure provides insight into the protein's conformation, function, and interactions with other molecules.......

    • • Thermal Shift Analysis

      Thermal shift analysis (TSA) is a technique employed to evaluate protein stability and ligand-binding characteristics by assessing protein conformational changes at varying temperatures. Typically, proteins denature upon heating, which induces measurable structural changes. Thermal shift analysis (TSA) leverages the interaction between fluorescent dyes and proteins; the fluorescence intensity of these dyes changes upon binding to denatured proteins. By examining the temperature-dependent fluorescence ......

    • • Protein Primary Sequence Analysis

      Protein primary sequence analysis is a vital component of proteomics research, involving the identification and interpretation of the amino acid sequences that make up protein molecules. Proteins consist of amino acids linked by peptide bonds, and their primary sequence denotes the linear order of these amino acids. Analyzing this sequence not only aids researchers in understanding the fundamental structure of proteins but also provides insights into their functions, folding mechanisms, and roles in b......

    • • Antibiotic Analysis

      Antibiotic analysis is a crucial experimental technique used to detect, quantify, and identify various antibiotics and their residual concentrations across different environments and sample types. This technique finds wide-ranging applications. In food safety monitoring, the issue of antibiotic residues in food products like meat and dairy, due to the extensive use of antibiotics in livestock farming, poses potential health risks to consumers. Antibiotic analysis effectively identifies these residues,......

    • • Protein Interaction Screening

      Protein interaction screening is a technological approach widely used in biological research to identify and analyze interactions between proteins. Proteins, as the executors of life activities, form complex networks through these interactions, thereby regulating various cellular functions, including signal transduction, metabolic pathway regulation, and cell cycle control. This approach is not only crucial for understanding the fundamental principles of life but also has important applications in stu......

    • • Amino Acid Sequencing Techniques

      Amino acid sequencing techniques refer to methods used to determine the amino acid sequence of a protein by analyzing its composition and arrangement. These techniques are widely applied in fields such as proteomics, disease diagnosis, drug development, and agricultural science. In proteomics, amino acid sequencing techniques help reveal the structure and function of proteins, providing insights into the molecular mechanisms that govern biological processes. In disease diagnosis, accurate amino acid s......

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